Isolation and antiproliferative activity of Lotus corniculatus lectin towards human tumour cell lines

Rafiq, Shaista; Majeed, Rabiya; Qazi, Asif Khurshid; Ganai, Bashir Ahmad; Wani, Ishfak Hussain; Rakhshanda, Syed; Qurishi, Yasrib; Sharma, P.R.; Hamid, Abid; Akbar, Muhammad; Hamid, Rizwan

doi:10.1016/j.phymed.2013.08.005

Cited by 35 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[6,7] In vitro studies have demonstrated good anti-inflammatory [8] and antimicrobial activity [9] of L. corniculatus, as well as its lectin-induced apoptotic activity in various human tumor cell lines. [10] In addition to that, the diet consisting of L. corniculatus increased reproductive efficiency in ewes. [11] Bioactive constituents in L. corniculatus include lipid antioxidants such as α-tocopherol, β-carotene, lutein [12] and oleanolic acid.…”

Section: Introductionmentioning

confidence: 97%

Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

Fumić

Jug

Končić

2019

Croat. chem. acta (Online)

View full text Add to dashboard Cite

Ultrasound-assisted extraction of phenolic antioxidants from Lotus corniculatus was optimized using response surface methodology. The extraction was performed according to the Box–Behnken design with ethanol concentration, temperature, and pH, as independent variables. The responses were extraction yield, DPPH radical scavenging activity (RSA) IC50 and content of different phenolic compounds (total phenols, flavonoids and phenolic acids, as well as quercetin, kaempferol and genistein derivatives). The models were used to calculate best conditions for maximal extraction of phenolic compounds and antiradical activity. Use of the optimized extraction parameters increased the content of quercetin and kaempferol derivatives more than tenfold (from 6.07 to 65.10 mg mL–1 and 6.69 to 92.75 mg mL–1, respectively). The results of this work stress the importance of careful selection of conditions for flavonoids extraction. Abundance of bioactive phenolics in L. corniculatus extracts obtained under optimized extraction conditions opens the possibility for wider utilization of this plant.

show abstract

Section: Introductionmentioning

confidence: 97%

Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

Fumić

Jug

Končić

2019

Croat. chem. acta (Online)

View full text Add to dashboard Cite

show abstract

“…Lotus corniculatus (Fabaceae) commonly known as Bird’s foot trefoil belongs to a genus that contains many dozens of species distributed worldwide e.g . Lotus aboriginus , Lotus angustissimus and Lotus argophyllus [ 4 ]. L. corniculatus is in the legume family and produces stem up to two feet in length.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory and Antioxidant Effects ofLotus corniculatuson Paracetamol-induced Hepatitis in Rats

Baali

Mezrag

Bouheroum

et al. 2020

AIAAMC

View full text Add to dashboard Cite

Background: Herbal medicines have been used in the treatment of liver diseases for a long time. The current study was elaborated to evaluate in vitro and in vivo antioxidant and anti-inflammatory effects of Lotus corniculatus (L. corniculatus) butanolic extract. Methods: The in vitro antioxidant and anti-inflammatory properties of L. corniculatus were investigated by employing DPPH radical scavenging, H2O2 scavenging and BSA denaturation assays. In vivo antioxidant and anti-inflammatory effects of L. corniculatus were evaluated against paracetamol (APAP)-induced hepatitis in rats. L.corniculatus at doses of 100 and 200 mg/kg was administered orally once daily for seven days. Serum transaminases (AST and ALT) and lactate dehydrogenase (LDH), total bilirubin levels, liver malondialdehyde (MDA), reduced glutathione (GSH), glutathione S- transferase (GST) and superoxide dismutase (SOD) levels and inflammatory markers, such as serum Creactive protein (CRP), circulating and liver myeloperoxidase (MPO) levels were investigated. Further histopathological analysis of the liver sections was performed to support the effectiveness of L. corniculatus. Results: L. corniculatus exhibited strong antioxidant and anti-inflammatory effects in vitro. In the in vivo study, our findings demonstrate that L. corniculatus (100 and 200 mg/kg) administration led to an amelioration of APAP effects on liver histology, liver functions parameters (AST, ALT, LDH, and total bilirubin levels) and liver oxidative stress markers (MDA, GSH, GST and SOD levels). Furthermore, serum CRP, circulating MPO and liver MPO levels were declined by both doses of L. corniculatus extract. The best benefits were observed with 200 mg/kg of L. corniculatus extract. Conclusion: Antioxidant and anti-inflammatory effects of L. corniculatus extract may be due to the presence of active components.

show abstract

“…For JC-1 staining, 250 μ L of PBS containing 2.5 μ g/mL JC-1 was added to the cells and incubated in the dark for 15 min. Cells were analyzed by a BD LSRFortessa Cell Analyzer [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

A Glucosamine-Specific Lectin from Green Dragon No. 8 Beans (Phaseolus vulgaris) Induced Apoptosis on Nasopharyngeal Carcinoma Cells

Chan

Xia

2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

A lectin exhibiting antiproliferative activity on tumor cell lines but devoid of antifungal activity has been purified from Phaseolus vulgaris cv. Green Dragon no. 8 seeds. The lectin was a 60 kDa dimeric protein with two 30 kDa subunits. It was a glucosamine-specific lectin as implied from the inhibitory effect of glucosamine on hemagglutinating activity of the lectin. The steps for isolation of the lectin involved Affi-gel blue gel (affinity gel), Mono Q (anion exchanger), and Superdex 75 column (size exclusion). The lectin was purified 20.8-fold from the crude extract of the beans. The purified lectin showed antiproliferative activity on breast cancer MCF7 cell line and nasopharyngeal cancer HONE1 and CNE2 cell lines, but a low activity on normal skin fibroblast HSF98 cell line. The lectin was shown to induce apoptosis on HONE1 cells, as indicated by increased phosphatidylserine externalization and mitochondrial depolarization. It also blocked HONE1 cell division and kept the cells at the G2/M phase of the cell cycle.

show abstract

Isolation and antiproliferative activity of Lotus corniculatus lectin towards human tumour cell lines

Cited by 35 publications

References 34 publications

Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

Anti-inflammatory and Antioxidant Effects ofLotus corniculatuson Paracetamol-induced Hepatitis in Rats

A Glucosamine-Specific Lectin from Green Dragon No. 8 Beans (Phaseolus vulgaris) Induced Apoptosis on Nasopharyngeal Carcinoma Cells

Contact Info

Product

Resources

About