Isolation and characterisation of dog uropathogenic Proteus mirabilis strains

Gaastra, Wim; Oosterom, R. A. A. van; Pieters, E. W. J.; Bergmans, Hans; Dijk, Linda van; Agnes, A G; Huurne, H. M. Ter

doi:10.1016/0378-1135(95)00133-6

Cited by 14 publications

(20 citation statements)

References 29 publications

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“…in animal intestines may pose a threat of autoinfection and cross-infection. The example of such autoinfection was described by Gaastra et al [44], who in the Netherlands isolated P. mirabilis strains from feces and urine of dogs suffering from recurrent urinary tract infections. It is probable that the intestine was a reservoir of the bacteria infecting the urinary tract of the dogs, because P. mirabilis strains were not isolated from feces of healthy controls.…”

Section: Proteus Spp In Animals—adverse and Friendlymentioning

confidence: 99%

Significance and Roles of Proteus spp. Bacteria in Natural Environments

2016

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Proteus spp. bacteria were first described in 1885 by Gustav Hauser, who had revealed their feature of intensive swarming growth. Currently, the genus is divided into Proteus mirabilis, Proteus vulgaris, Proteus penneri, Proteus hauseri, and three unnamed genomospecies 4, 5, and 6 and consists of 80 O-antigenic serogroups. The bacteria are known to be human opportunistic pathogens, isolated from urine, wounds, and other clinical sources. It is postulated that intestines are a reservoir of these proteolytic organisms. Many wild and domestic animals may be hosts of Proteus spp. bacteria, which are commonly known to play a role of parasites or commensals. However, interesting examples of their symbiotic relationships with higher organisms have also been described. Proteus spp. bacteria present in soil or water habitats are often regarded as indicators of fecal pollution, posing a threat of poisoning when the contaminated water or seafood is consumed. The health risk may also be connected with drug-resistant strains sourcing from intestines. Positive aspects of the bacteria presence in water and soil are connected with exceptional features displayed by autochthonic Proteus spp. strains detected in these environments. These rods acquire various metabolic abilities allowing their adaptation to different environmental conditions, such as high concentrations of heavy metals or toxic substances, which may be exploited as sources of energy and nutrition by the bacteria. The Proteus spp. abilities to tolerate or utilize polluting compounds as well as promote plant growth provide a possibility of employing these microorganisms in bioremediation and environmental protection.

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Section: Proteus Spp In Animals—adverse and Friendlymentioning

confidence: 99%

Significance and Roles of Proteus spp. Bacteria in Natural Environments

2016

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“…However, the same authors suggest that expression of urease, a major uropathogenic P. mirabilis virulence factor, is present in all isolates from any source (Mobley & Chippendale, 1990). Gaastra et al (1996) assessed the presence and expression of genes of MR/P and uroepithelial cell adhesion (UCA) fimbriae, and production of haemolysin in P. mirabilis strains isolated from the urine and faeces of dogs with UTI. These authors observed that all but one of the P. mirabilis strains were haemolytic and that most strains produced fimbriae.…”

Section: Introductionmentioning

confidence: 99%

Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

2006

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Proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing an important role in urinary tract infections (UTIs). In this study, a collection of P. mirabilis isolates obtained from clinical and non-clinical sources was analysed in order to determine a possible correlation between origin, virulence factors and in vivo infectivity. Isolates were characterized in vitro, assessing several virulence properties that had been previously associated with P. mirabilis uropathogenicity. Swarming motility, urease production, growth in urine, outer-membrane protein patterns, ability to grow in the presence of different iron sources, haemolysin and haemagglutinin production, and the presence and expression of diverse fimbrial genes, were analysed. In order to evaluate the infectivity of the different isolates, the experimental ascending UTI model in mice was used. Additionally, the Dienes test and the enterobacterial repetitive intergenic consensus (ERIC)-PCR assay were performed to assess the genetic diversity of the isolates. The results of the present study did not show any correlation between distribution of the diverse potential urovirulence factors and isolate source. No significant correlation was observed between infectivity and the origin of the isolates, since they all similarly colonized the urinary tract of the challenged mice. Finally, all isolates showed unique ERIC-PCR patterns, indicating that the isolates were genetically diverse. The results obtained in this study suggest that the source of P. mirabilis strains cannot be correlated with pathogenic attributes, and that the distribution of virulence factors between isolates of different origins may correspond to the opportunistic nature of the organism.

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“…In both hosts, the majority of UTI is caused by Escherichia coli and about 10% by P. mirabili~ (Gaastra et al, 1995). To exert their pathogenicity, P. mirabilis strains are equipped with 0001-9484 0 1995 SGM…”

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Nucleotide sequences of two fimbrial major subunit genes, pmpA and ucaA, from canine-uropathogenic Proteus mirabilis strains

et al. 1995

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Proteus mirabilis strains were isolated from dogs with urinary tract infection (UTI) and fimbriae were prepared from two strains. The N-terminal amino acid sequences of the major fimbrial subunits were determined and both sequences appeared identical to the N-terminal amino acid sequence of a urinary cell adhesin (UCA) (Wray, S. K., Hull, S. I., Cook, R. G., Barrish, J. & Hull, R. A., 1986, Infect Immun 54, 43-49). The genes of two different major fimbrial subunits were cloned using oligonucleotide probes that were designed on the basis of the N-terminal UCA sequence. Nucleotide sequencing revealed the complete ucaA gene of 540 bp (from strain IVB247) encoding a polypeptide of 180 amino acids, including a 22 amino acid signal sequence peptide, and the pmpA (P. mirabilis P-like pili) gene of 549 bp (from strain IVB219) encoding a polypeptide of 183 amino acids, including a 23 amino acid signal sequence. Hybridization experiments gave clear indications of the presence of both kinds of fimbriae in many UTI-related canine P. mirabilis isolates. However, the presence of these fimbriae could not be demonstrated in P. vulgaris or other Proteus-related species. Database analysis of amino acid sequences of major subunit proteins revealed that the UcaA protein shares about 56% amino acid identity with the F17A and F111A major fimbrial subunits from bovine enterotoxigenic Escherichia coli. In turn, the PmpA protein more closely resembled the pyelonephritis-associated pili (Pap)-like major subunit protein from UTI-related E. coli. The evolutionary relationship of UcaA, PmpA and various other fimbrial subunit proteins is presented in a phylogenetic tree.

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Isolation and characterisation of dog uropathogenic Proteus mirabilis strains

Cited by 14 publications

References 29 publications

Significance and Roles of Proteus spp. Bacteria in Natural Environments

Significance and Roles of Proteus spp. Bacteria in Natural Environments

Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

Nucleotide sequences of two fimbrial major subunit genes, pmpA and ucaA, from canine-uropathogenic Proteus mirabilis strains

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