Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle

Wang, Bin; Gong, Yandan; Li, Zhongrui; Di, Yu; Chi, Chang-Feng; Ma, Jun

doi:10.1016/j.jff.2013.10.004

Cited by 89 publications

(54 citation statements)

References 30 publications

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“…Marine animal tissues rich in proteins, such as skin, muscle, bone, and other by-product, are materials used most commonly in preparation of potential antioxidant peptides. In the last decade, researchers have successfully isolated effective antioxidant peptides from the hydrolysate of blacktip shark skin gelatin [31], croceine croaker skin gelatin [32], Otolithes ruber muscle [33], monkfish (Lophius litulon) [34], round scad (Decapterus maruadsi) [35], Mustelus griseus [36], and by-products of popular seafood like tuna skin [37], salmon pectoral fin [38], and heads and viscera of sardinelle [39]. It is reported that salmon pectoral fin hydrolyzed by pepsin can release antioxidant peptide (Phe-LeuGln-Glu-Phe-Leu-His-Val) in Ahn et al's research [38].…”

Section: Marine Sources Of Antioxidant Peptidesmentioning

confidence: 99%

“…This property may improve the radical scavenging activity of peptides [36]. Trytophan and tyrosine donate hydrogen atom to scavenge radicals and the indole group or hydroxyphenyl group will change into indole radicals or phenoxy radicals, and they can stabilize themselves by forming the electron-conjugated system.…”

Section: The Composition Of Amino Acidsmentioning

confidence: 99%

“…Hydroxyl radical (·OH) is the most active oxygen radical, which can attack the C-H bond of many substances in cells to form H 2 O and organic radical [36], causing radical chain reaction and inducing severe damage to cells. Hydroxyl radical belongs to the reactive oxygen species in vivo.…”

Section: Hydroxyl Radical Scavenging Assaymentioning

confidence: 99%

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Overview of Antioxidant Peptides Derived from Marine Resources: The Sources, Characteristic, Purification, and Evaluation Methods

Wü

Liú

et al. 2015

Appl Biochem Biotechnol

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Marine organisms are rich sources of structurally diverse bioactive nitrogenous components. In recent years, numerous bioactive peptides have been identified in a range of marine protein resources, such as antioxidant peptides. Many studies have approved that marine antioxidant peptides have a positive effect on human health and the food industry. Antioxidant activity of peptides can be attributed to free radicals scavenging, inhibition of lipid peroxidation, and metal ion chelating. Moreover, it has also been verified that peptide structure and its amino acid sequence can mainly affect its antioxidant properties. The aim of this review is to summarize kinds of antioxidant peptides from various marine resources. Additionally, the relationship between structure and antioxidant activities of peptides is discussed in this paper. Finally, current technologies used in the preparation, purification, and evaluation of marine-derived antioxidant peptides are also reviewed.

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Section: Marine Sources Of Antioxidant Peptidesmentioning

confidence: 99%

Section: The Composition Of Amino Acidsmentioning

confidence: 99%

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Overview of Antioxidant Peptides Derived from Marine Resources: The Sources, Characteristic, Purification, and Evaluation Methods

Wü

Liú

et al. 2015

Appl Biochem Biotechnol

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“…Numerous antioxidant compounds, particularly fish peptide, have been widely studied. Potent antioxidant peptides have been isolated from protein hydrolysates from the muscle of yellow stripe trevally (Selaroides leptolepis) (Klompong et al 2009), cod (Gadus morhua) (Halldorsdottir et al 2014) and spotless smooth hound (Mustelus griseus) (Wang et al 2014), etc. Protein, peptides and free amino acids released during enzymatic hydrolysis are capable of modulating specific biological function.…”

Section: Introductionmentioning

confidence: 99%

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

et al. 2015

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Antioxidant activities of protein hydrolysate prepared from Nile tilapia protein isolate using Alcalase (HA), Alcalase followed by papain (HAPa) and their Sephadex G-25 fractions (FHA and FHAPa) were investigated in both chemical and cellular based models. Amongst all samples, FHAPa showed the highest chemical antioxidant activities, however it had no metal chelation activity. Cellular antioxidant ability of HA, HAPa and their fractions against H 2 O 2 and AAPH induced oxidative damage of HepG2 cell and DNA were tested. When cells were pretreated with all hydrolysates or fractions at different concentrations (0.5-2 mg/mL) in the absence and presence of 50 μM Trolox, cell viability was in the range of 91.10-111.40 %. However, no difference in cell viability was observed among samples having various concentrations (P>0.05). Cell reactive oxygen species (ROS) generation as mediated by H 2 O 2 and AAPH decreased with treatment of hydrolysates or their fractions, especially in combination with 50 μM Trolox. FHAPa effectively inhibited H 2 O 2 and peroxyl radical induced DNA scission in a dose dependent manner. Therefore, Nile tilapia protein hydrolysates could serve as a functional food ingredient.

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“…It should be noted that the gelatinolytic activity of MMP-13 is about 44 times greater than that of MMP-1, and that it plays an important role in extra-cellulose matrix degradation [10]. Techniques such as ultracentrifugation, molecular cut-off filtration, ultrafiltration and chromatography were needed to separate small peptides (ultra-peptides), especially those with molecular weights <1500 Da [11]. Additionally, these peptides could be separated by using urea-sodium dodecyl sulfate (SDS) -polyacrylamide gels [12] or tricine -SDS -polyacrylamide gels [13].…”

Section: Resultsmentioning

confidence: 99%

Peptide Hydrolysates from Astragalus membranaceus Bunge Inhibit the Expression of Matrix Metalloproteinases in Human Dermal Fibroblasts

Park¹,

Hien²,

Luong³

et al. 2014

KSBB Journal

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Inhibition effects of peptide hydrolysates from Astragalus membranaceus Bunge. on the expression of the matrix metalloproteinases (MMPs) in human dermal fibroblasts were evaluated in vitro. Crude peptides were obtained by the hydrolysis of proteins extracted from A. membranaceus. Peptides were purified partially by the basis on the molecular weight using 40% polyacrylamide gel electrophoresis before treatment with human dermal fibroblasts. Basis on the doseeffect experiments, expressions of MMPs including MMP-1, MMP-3, MMP-8, MMP-13 in human dermal fibroblasts were evaluated. Expressions of MMP-1, MMP-3, MMP-8 and MMP-13 were reduced in 43%, 5%, 22% and 57% respectively. The mass spectrometric analysis of partially purified peptides from A. membranaceus, which strongly inhibit expressions of MMPs, indicated that the peptides were composed of molecules below 1500 Da.

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Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle

Cited by 89 publications

References 30 publications

Overview of Antioxidant Peptides Derived from Marine Resources: The Sources, Characteristic, Purification, and Evaluation Methods

Overview of Antioxidant Peptides Derived from Marine Resources: The Sources, Characteristic, Purification, and Evaluation Methods

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

Peptide Hydrolysates from Astragalus membranaceus Bunge Inhibit the Expression of Matrix Metalloproteinases in Human Dermal Fibroblasts

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