Isolation and characterisation of metallothionein from the clam Ruditapes decussatus

Simes, Dina C.; Bebianno, Maria João; Moura, José J. G.

doi:10.1016/s0166-445x(02)00185-6

Cited by 32 publications

(16 citation statements)

References 36 publications

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“…Many MT isoforms have been identified in different marine organisms [30]. For the remaining peaks, their elution times and shapes remained comparable to those of the controls.…”

Section: Separation Of Metal-binding Protein Ligands Using Hplcmentioning

confidence: 67%

Transfer and efflux of cadmium and silver in marine snails and fish fed pre‐exposed mussel prey

Cheung

Zhang

Wang

2007

Enviro Toxic and Chemistry

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Subcellular metal distribution may play an important role in the bioaccumulation and trophic transfer of metals in marine food chains. In the present study, we preexposed the green mussel Perna viridis to Ag and Cd and quantified their trophic transfer efficiencies to two predators (whelks [Thais clavigera] and fish [Terapon jarbua]). For the mussels, more Ag was distributed in the metal-rich granule (MRG) fraction following Ag exposure, and more Cd was distributed in the metallothionein-like protein following Cd exposure. In addition, Cd was mainly bound with the proteins having a molecular size of approximately 20 kDa. After being fed with metal-exposed mussels, the assimilation efficiencies of Ag decreased significantly (from 77 to 29-60% in whelks and from 9 to 2% in fish) with an increasing percentage of Ag deposited in the MRG fraction of the prey. In contrast, the assimilation efficiencies of Cd remained comparable (81-85% in whelks and 6-8% in fish), because its partitioning in the soluble fraction of different treatments of the prey was similar. The efflux of Ag and Cd in the two predators was comparable after feeding on preexposed mussel prey. Our results imply that the subcellular distribution of metals in prey may affect the dietary assimilation of metals in predators, but such influence is clearly metal-specific. The present study may lead to a better understanding of metal trophic transfer in different marine food chains.

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“…Many MT isoforms have been identified in different marine organisms [30]. For the remaining peaks, their elution times and shapes remained comparable to those of the controls.…”

Section: Separation Of Metal-binding Protein Ligands Using Hplcmentioning

confidence: 67%

Transfer and efflux of cadmium and silver in marine snails and fish fed pre‐exposed mussel prey

Cheung

Zhang

Wang

2007

Enviro Toxic and Chemistry

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“…The different expressions of MTs may be linked to their biochemical and physiological functions. Additionally, metallothioneins act as chelators of heavy metal, they are essential for metal homeostasis and detoxification, and they have important functions in the elimination of intracellular free radicals . Also, the thiol groups in metallothioneins can act as powerful antioxidants so MTs may have roles in protection against oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

“…Plant metallothioneins have been subdivided into four categories based on the arrangement of the cysteine residues along the peptide chain. These proteins have different amino acid compositions, isoelectric points and hydrophobic properties . Type 1 MTs have Cys‐Xaa‐Cys motifs in their N‐ and C‐ terminal regions; type 2 have Cys‐Cys, Cys‐Xaa‐Cys and Cys‐Xaa‐Xaa‐Cys motifs at the N‐ terminal domain and Cys‐Xaa‐Cys motifs in the C‐terminal domain.…”

Section: Introductionmentioning

confidence: 99%

Expression analysis of metallothioneins and mineral contents in tomato (Lycopersicon esculentum) under heavy metal stress

Kısa

Öztürk

Döker

et al. 2016

J. Sci. Food Agric.

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“…In the case of mollusks MT have a high glycine content differentiating them from most of the other MT isoforms known today . MTs are distinctively identified in only a few species of marine molluscs (Patella vulgata, C. virginica, Patella granularis, Mytilus edulis) (Isani et al 2000;Mourgaud et al 2002) and recently in Ruditapes decussates (Simes et al 2003) and fish (Zhang and Wang 2005). The induction of MT has been detected in organisms from contaminated areas or in vitro experiments with exposure to metals such as Ag, Cd, Cu, Hg and Zn in laboratory.…”

Section: Metallothionein Inductionmentioning

confidence: 99%

Molecular Biomarkers: Their significance and application in marine pollution monitoring

et al. 2006

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This paper presents an overview of the significance of the use of molecular biomarkers as diagnostic and prognostic tools for marine pollution monitoring. In order to assess the impact of highly persistent pollutants such as polychlorinated biphenyls (PCB), polychlorinated dibenzo-dioxins (PCDD), polychlorinated dibenzo-furans (PCDF), polynuclear aromatic hydrocarbons (PAH), tributyltin (TBT) and other toxic metals on the marine ecosystem a suite of biomarkers are being extensively used worldwide. Among the various types of biomarkers, the following have received special attention: cytochrome P4501A induction, DNA integrity, acetylcholinesterase activity and metallothionein induction. These biomarkers are being used to evaluate exposure of various species of sentinel marine organisms (e.g. mussels, clams, oysters, snails, fishes, etc.) to and the effect of various contaminants (organic xenobiotics and metals) using different molecular approaches [biochemical assays, enzyme linked immuno-sorbent assays (ELISA), spectrophotometric, fluorometric measurement, differential pulsed polarography, liquid chromatography, atomic absorption spectrometry]. The induction of the biotransformation enzyme, cytochrome P4501A in fishes (Callionymus lyra, Limanda limanda, Serranus sp., Mullus barbatus) and mussels (Dreissena polymorpha) by various xenobiotic contaminants such as PCBs, PAHs, PCDs is used as a biomarker of exposure to such organic pollutants. The induction of cytochrome P4501A is involved in chemical carcinogenesis through catalysis of the covalent bonding of organic contaminants to a DNA strand leading to formation of DNA adduct. Measurement of the induction of cytochrome P4501A in terms of EROD (7-ethoxy resorufin O-deethylase) activity is successfully used as a potential biomarker of exposure to xenobiotic contaminants in marine pollution monitoring. In order to assess the impact of neurotoxic compounds on marine environment the evaluation of acetylcholinesterase activity in marine organisms is used as a biomarker of exposure to neurotoxic agents such as organophosphorus, carbamate pesticides etc. Metallothioneins (MTs) are induced by toxic metals such as Cd, Hg, and Cu by chelation through cysteine residues and are used in both vertebrates and invertebrates as a biomarker of metal exposure. The measurement of the levels of DNA integrity in marine organisms such as Sea stars (Asterias rubens) from the North Sea and the marine snails (Planaxis sulcatus) from the Arabian Sea along the Goa coast exposed to environmental xenobiotic contaminants clearly indicated the extent and the nature of pollution at the sampling sites along coastal environment.

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Isolation and characterisation of metallothionein from the clam Ruditapes decussatus

Cited by 32 publications

References 36 publications

Transfer and efflux of cadmium and silver in marine snails and fish fed pre‐exposed mussel prey

Transfer and efflux of cadmium and silver in marine snails and fish fed pre‐exposed mussel prey

Expression analysis of metallothioneins and mineral contents in tomato (Lycopersicon esculentum) under heavy metal stress

Molecular Biomarkers: Their significance and application in marine pollution monitoring

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