Isolation and characterisation of nuv11, a mutation affecting meiotic and mitotic recombination in Aspergillus nidulans

Osman, Fekret; Cotton, Catherine; Tomsett, Brian; Strike, Peter

doi:10.1016/0300-9084(91)90219-q

Cited by 4 publications

(11 citation statements)

References 19 publications

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“…Control transformants with the vector alone in L605 and also in the isogenic wild-type strain, L604, were created. The MNNG and 4NQO sensitivities of these haploid transformants were tested and found to be the same as the original transformants (Osman et al ., 1991) (data not shown). Sexual crosses were carried out and diploids made with the new transformants and the nuvA 11 strain, L603, which contains the linkage group II markers wA3 and puA1 plus the niaD allele, niiAniaDA608, the other deletion required for the intragenic recombination assays (Osman et al ., 1991).…”

Section: Resultsmentioning

confidence: 99%

“…A 40 kb A. nidulans genomic DNA clone, pNUV11A40 (), that restored the MNNG and 4NQO sensitivities of the nuv A11 mutant strain, L601, was described previously (Osman et al ., 1991). The resistance of strain L601 transformed with pNUV 11A40 was shown to be the same as that of an equivalent wild-type strain, L600, transformed with just the vector, pCAP2, whilst L601 remained hypersensitive to both agents when transformed with vector alone.…”

Section: Resultsmentioning

confidence: 99%

“…To determine its effects on recombination, pNUVll A40 was used to transform the nuvA 11 mutant strain L605 which contains the yA2 and luA1 markers on chromosome I and can be used for assessing intergenic recombination frequencies. It also carries the niaD26 deletion on chromosome VIII, one of the two niaD alleles used in intragenic recombination assays (Osman et al ., 1991). Control transformants with the vector alone in L605 and also in the isogenic wild-type strain, L604, were created.…”

Section: Resultsmentioning

confidence: 99%

“…In a previous paper (Osman et al ., 1991) we reported the isolation of the A. nidulans nuv A11 mutation, a recessive, single gene defect on linkage group IV, originally identified as causing sensitivity to the DNA damaging agents N -methyl- N′ -nitro- N -nitrosoguanidine (MNNG) and 4-nitroquinolene-l-oxide (4NQO). The nuv A11 mutants were shown to have a complex phenotype.…”

Section: Introductionmentioning

confidence: 92%

“…The nuv A11 mutation also results in sensitivity to UV and γ irradiation and higher UV-induced mutation frequencies, but not γ -ray-induced mutation frequencies (S. May, this laboratory, unpublished observations). The nuv A11 mutant phenotype was previously compared (Osman et al ., 1991) with those of the S. cerevisiae RAD52 epistasis group mutants rad50 and rad52 which are defective in their repair of double-strand breaks (Game, 1983; Haynes & Kunz, 1981). Although similar, none of the phenotypes were identical to that caused by the nuvA 11 allele.…”

Section: Introductionmentioning

confidence: 99%

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nuvA, An Aspergillus Nidulans Gene Involved in DNA Repair and Recombination, is a Homologue of Saccharomyces Cerevisiae RAD18 and Neurospora Crassa uvs-2

et al. 1996

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A 40 kb genomic clone and 2 3 kb EcoRl subclone that rescued the DNA repair and recombination defects of the Aspergillus nidulans nuvA11 mutant were isolated and the subclone sequenced. The subclone hybridized to a cosmid in a chromosome-specif ic library confirming the assignment of nuwA to linkage group IV and indicating its closeness to bimD. Amplification by PCR clarified the relative positions of nuwA and bimD. A region identified within the subclone, encoding a C3HC4 zinc finger motif, was used as a probe to retrieve a cDNA clone. Sequencing of this clone showed that the nuwA gene has an ORF of 1329 bp with two introns of 51 bp and 60 bp. Expression of nuvA appears to be extremely low. The putative NUVA polypeptide has two zinc finger motifs a molecular mass of 48906 Da and has 39% identity with the Neumspora crassa uws-2 and 25% identity with the Sacchammyces cerevisiae WID18 translation products. Although mutations in nuvA, urn-2 and RAD18 produce similar phenotypes, only the nuvA11 mutation affects meiotic recombination. A role for nuvA in both DNA repair and genetic recombination is proposed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

nuvA, An Aspergillus Nidulans Gene Involved in DNA Repair and Recombination, is a Homologue of Saccharomyces Cerevisiae RAD18 and Neurospora Crassa uvs-2

et al. 1996

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Recombinagenic effect of cryptolepine inuvsH +//uvsH + anduvsH//uvsH diploid strains ofAspergillus nidulans

Chiuchetta

Castro-Prado

2002

Folia Microbiol

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Recombinagenic potential of the alkaloid cryptolepine was evaluated in two diploid strains of Aspergillus nidulans--a wild type strain (uvsH+//uvsH+) and a DNA-repair-deficient one (uvsH//uvsH). Treatment of both strains with cryptolepine failed to alter colony growth in culture; its recombinagenic potential was determined by the homozygotization index (in which events of mitotic exchange may cause expression of genes previously masked by the dominant allele). Mitotic crossing-overs were induced by 7 and 14 mg/L doses of cryptolepine in both diploid A. nidulans strains.

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The isolation of mutagen-sensitive nuv mutants of Aspergillus nidulans and their effects on mitotic recombination.

1993

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More than 200 mutants of Aspergillus nidulans were isolated as hypersensitive to the monofunctional alkylating agent MNNG and/or UV-irradiation (designated nuv mutants). Of these, 23 were selected for further characterization. All were markedly hypersensitive to both MNNG and the quasi-UV-mimetic mutagen 4-NQO. The hypersensitive phenotype of each mutant was shown to result from mutation of a single gene. The nuv mutants exhibited a diverse range of growth responses on solid media containing various concentrations of MNNG or 4-NQO. This suggested that they represented many nonallelic mutations. Analysis to determine the dominance/recessiveness of the nuv mutations with respect to hypersensitivity revealed that most were fully recessive, although several appeared to be semidominant. A novel system to assay homologous mitotic recombination using simple plating tests was developed. The system was exploited to determine the effects of the nuv mutations on mitotic recombination. Of the 23 mutations tested, 10 caused a hypo-recombination phenotype and three a hyper-recombination phenotype, while 10 appeared to have no effect on recombination. The hypo-rec effect of one of the mutations, nuv-117, appeared to be semidominant. Transcomplementation analysis between seven of the nuv mutations defined at least six nonallelic loci.

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Isolation and characterisation of nuv11, a mutation affecting meiotic and mitotic recombination in Aspergillus nidulans

Cited by 4 publications

References 19 publications

nuvA, An Aspergillus Nidulans Gene Involved in DNA Repair and Recombination, is a Homologue of Saccharomyces Cerevisiae RAD18 and Neurospora Crassa uvs-2

nuvA, An Aspergillus Nidulans Gene Involved in DNA Repair and Recombination, is a Homologue of Saccharomyces Cerevisiae RAD18 and Neurospora Crassa uvs-2

Recombinagenic effect of cryptolepine inuvsH +//uvsH + anduvsH//uvsH diploid strains ofAspergillus nidulans

The isolation of mutagen-sensitive nuv mutants of Aspergillus nidulans and their effects on mitotic recombination.

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