Isolation and characterisation of Rhodococcus equi from submaxillary lymph nodes of wild boars (Sus scrofa)

Makrai, László; Kobayashi, Akito; Matsuoka, Misa; Sasaki, Yukako; Kakuda, Tsutomu; Jankovics, I.; Hajtós, I.; Révész, István; Jánosi, Katalin; Fodor, László; Várga, J.; Takaı̈, Shinji

doi:10.1016/j.vetmic.2008.04.009

Cited by 30 publications

(38 citation statements)

References 27 publications

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“…The abovementioned Hungarian study (Makrai et al, 2005) reported six plasmid types in 26.8% VapB-positive isolates. Furthermore, VapB-positive R. equi was isolated with a similar isolation rate (25.6%) also from submaxillary lymph nodes of Hungarian wild boars (Makrai et al, 2008). The isolation of strains containing a 95-kb plasmid type 5 in pigs, wild boars and immunocompromised humans supports the hypothesis that there is an epidemiological relationship between human R. equi infections and the presence of intermediately virulent R. equi strains in pigs and wild boars (Makrai et al, 2005(Makrai et al, , 2008.…”

Section: Discussionsupporting

confidence: 59%

“…Thus, only nine strains could be investigated for the presence of large plasmids and five plasmid types were identified with type 5 being the most prevalent. The same plasmid type has been found in the majority of pig and wild boar isolates in Hungary (Makrai et al, 2005(Makrai et al, , 2008 but in a much lower proportion (2.9-9.9%) in isolates from Japan (Fukunaga et al, 1999). In addition, four isolates exhibited different restriction patterns from the ones discovered before.…”

Section: Discussionmentioning

confidence: 53%

“…Of the representative plasmid types, eight have been found in Japanese pig isolates (types 2-4, 6-9 and 12; Fukunaga et al, 1999), eight in human isolates from France and Thailand (types 1, 5, 10, 11, 13-16; Takai et al, 1995), one in a Hungarian pig isolate (type 17; Makrai et al, 2005), three in pig isolates from Thailand (types 18,22,23;Takai,personal communication) and three in wild boar isolates from Hungary (types 25-27; Makrai et al, 2008).…”

Section: Discussionmentioning

confidence: 95%

“…Intermediately virulent strains are characterized by the presence of a virulence-associated 20-kDa antigen (VapB) and virulence plasmids of 79 to 100 kb found in pig, wild boar and human isolates; 23 distinct plasmid types have been reported to date (Takai et al, 1995;Fukunaga et al, 1999;Makrai et al, 2008). Avirulent R. equi contains neither virulence-associated antigens nor plasmid DNA and is widespread in soil (Takai, 1997).…”

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confidence: 99%

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Intermediately virulent Rhodococcus equi isolates from pigs in Slovenia: discovery of new plasmid types and assessment of genetic diversity by pulsed-field gel electrophoresis

Pate¹,

Ocepek²,

Zdovc³

et al. 2009

Vet. Med. - Czech

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ABSTRACT:The presence of large plasmids in 30 Rhodococcus equi strains isolated from pig lymph nodes with granulomatous changes was investigated. Plasmid DNAs were isolated and digested with the restriction endonucleases BamHI, EcoRI, EcoT22I and HindIII for detailed comparison and estimation of plasmid sizes. A total of nine isolates were identified as intermediately virulent (VapB-positive), harbouring large plasmids of type 5 (n = 5) and four new variants that we tentatively designated as type 19 (n = 1), 20 (n = 1), 21 (n = 1) and 24 (n = 1). All isolates were subjected to genotyping with pulsed-field gel electrophoresis (PFGE). High genetic diversity was observed: 21 distinct genotypes were detected; five were found in multiple isolates and the others were unique. Isolates of the same plasmid type exhibited different PFGE profiles and vice versa. In a few cases, multiple strains from certain farms were analysed, the majority of which exhibited diverse PFGE profiles. Our findings demonstrate the presence of a wide variety of R. equi strains even in small confined environments such as farms. This is the first molecular epidemiology study of intermediately virulent R. equi isolates from Slovenian pigs.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

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Intermediately virulent Rhodococcus equi isolates from pigs in Slovenia: discovery of new plasmid types and assessment of genetic diversity by pulsed-field gel electrophoresis

Pate¹,

Ocepek²,

Zdovc³

et al. 2009

Vet. Med. - Czech

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“…Corresponding plasmid profiles and virulence levels of reference strains have been described. 2,6,7,9,10 Statistical analysis was conducted by using the chi-square test (Epi Info version 6.4; Centers for Disease Control and Prevention, Atlanta, GA), and P < 0.05 was considered significant.…”

mentioning

confidence: 99%

Identification of Virulence-Associated Plasmids in Rhodococcus equi in Humans with and without Acquired Immunodeficiency Syndrome in Brazil

Ribeiro¹,

Takaı̈²,

Vargas³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Virulence of Rhodococcus equi strains from 20 humans in Brazil was investigated by using a polymerase chain reaction to characterize isolates as virulent (VapA), intermediately virulent (VapB), and avirulent. Nine isolates were obtained from human immunodeficiency virus (HIV)–positive patients, six from HIV-negative patients, and five from patients of unknown status. Five isolates were VapB positive, four were VapA positive, and eleven were avirulent. Among the nine isolates from HIV-positive patients, five contained VapB plasmids and two contained VapA plasmids. Five VapB-positive isolates had the type 8 virulence plasmid. Eleven of the patients had a history of contact with livestock and/or a farm environment, and none had contact with pigs.

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Other Bacterial Infections

Handeland

Speck²,

Birtles

et al. 2012

Infectious Diseases of Wild Mammals and Birds in Europe

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Isolation and characterisation of Rhodococcus equi from submaxillary lymph nodes of wild boars (Sus scrofa)

Cited by 30 publications

References 27 publications

Intermediately virulent Rhodococcus equi isolates from pigs in Slovenia: discovery of new plasmid types and assessment of genetic diversity by pulsed-field gel electrophoresis

Intermediately virulent Rhodococcus equi isolates from pigs in Slovenia: discovery of new plasmid types and assessment of genetic diversity by pulsed-field gel electrophoresis

Identification of Virulence-Associated Plasmids in Rhodococcus equi in Humans with and without Acquired Immunodeficiency Syndrome in Brazil

Other Bacterial Infections

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