Isolation and characteristics of multi-drug resistant Streptococcus porcinus from the vaginal secretions of sow with endometritis

Abstract

Background: Sow endometritis is a common disease in pig breeding farms after artificial insemination, which leads to gray-green vaginal secretions and decreased conception rates. It is important to perform an etiologic diagnosis for effective treatments and control of diseases. The aim of this study was to carry out a pathogenic detection in five specimens of vaginal secretions collected from sick pigs with endometritis, implement identification of the pathogens by phenotypic detection and 16 s rDNA sequence and phylogeny analysis, and determinate antibiotic susceptibility of the isolates. Results: A Streptococcus strain was isolated and identified from all of the five specimens. The isolate was positive for Voges-Proskauer (V-P) and for the hydrolysis of arginine, esculin and myelin-associated glycoprotein (MAG). Acid formation was observed for sorbitol, mushroom sugar, sucrose, and glucose. The 16S rDNA sequence of the isolate possessed 99.93% similarity to that of Streptococcus porcinus. The phylogenetic analysis of 16S rDNA sequence showed that the isolate belonged to the same clade as the S. porcinus strains from humans, pigs, and other animals. The isolate exhibited multi-drug resistance to aminoglycosides, quinolones, macrolides and tetracyclines except being sensitive to some βlactams such as penicillin G, cephalothin, cefazolin, cephradine and cefuroxime. Conclusions: A S. porcinus isolate with multi-drug resistance was identified from vaginal secretions of sows with endometritis in one pig breeding farm, which suggests that the sow endometritis was caused by S. porcinus infection during artificial insemination. This study indicates that sensitive antibiotics such as penicillin G or some cephalosporins could be used for treatment of the diseases. In addition, the study hints that bacterial multi-drug resistance is a tough problem for disease treatment in pig farms.

Detection of Various Streptococcus spp. and Their Antimicrobial Resistance Patterns in Clinical Specimens from Austrian Swine Stocks

“…Thus, hardly any investigations on S. porcinus have been published recently. In contrast to previous reports [ 26 ], we could not recover S. porcinus from the CNS, urogenital tract or joints.…”

Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil

“…It is also related to cases of endometritis in swine after artificial insemination in farms. Antibiotic resistance is prevalent in this species, with some strains resistant to erythromycin, streptomycin, tetracycline, aminoglycosides, quinolones, macrolides, and others [ 56 ].…”

Active components and molecular mechanism of Syringa oblata Lindl. in the treatment of endometritis based on pharmacology network prediction

“…It not only causes infertility in humans and animals but also leads to economic losses due to unfavorable effects on survival, productivity, and animal welfare (2,3). It is reported that in some large farms of China, 47.6% of sows were culled due to various abnormalities, of which 41.4% were culled due to endometritis, and even severe endometritis in sow can also cause sepsis and death (4,5). The causes of endometritis are very complex.…”