Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China

Zhao, Xing; Sun, Weibang; Yang, Jun‐Bo; Meng, Jing

doi:10.1007/s10592-008-9799-3

Cited by 9 publications

(4 citation statements)

References 5 publications

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“…Similar to other endangered Magnoliaceae species (Zhao et al, 2009; Sun et al, 2010), substantial genetic diversity was found in the investigated populations of T. odorum . The battery of microsatellite markers presented here provides a powerful tool for further investigation of the genetic structure, mating system, and gene flow of T. odorum , which are highly valuable for formulating conservation strategies.…”

Section: Discussionsupporting

confidence: 75%

Isolation and characterization of microsatellite loci in Tsoongiodendron odorum (Magnoliaceae)

Jiang

Wang

Kang

et al. 2011

American J of Botany

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Section: Discussionsupporting

confidence: 75%

Isolation and characterization of microsatellite loci in Tsoongiodendron odorum (Magnoliaceae)

Jiang

Wang

Kang

et al. 2011

American J of Botany

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“…Michelia coriacea is diploid (2 n = 38) [ 39 ]. The mature trees can reach up to 20–30 m in height and 50–60 cm in diameter [ 18 , 19 , 40 ], and live for at least 100 years. The species bears scented whitish or creamy yellow flowers with 6–7 tepals which are in anthesis from February to April [ 13 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…SSR genotyping was performed according to the methods described in Zhao et al [ 40 ] at loci MC2, MC3, MC8, MC34, MC35, MC41, MC45, MC48, MC49, MC64, and MC66 ( Table 2 ). Briefly, SSR loci were amplified by PCR in a final volume of 15 μL containing 7.5 μL 2× Taq PCR MasterMix (Tiangen; 0.1 U Taq Polymerase/μL, 0.5 mM dNTP each, 20 mM Tris-HCl, 100 mM KCl, 3 mM MgCl 2 ), 20–50 ng genomic DNA.…”

Section: Methodsmentioning

confidence: 99%

High Genetic Diversity and Low Differentiation of Michelia coriacea (Magnoliaceae), a Critically Endangered Endemic in Southeast Yunnan, China

Zhao

Sun

et al. 2012

IJMS

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Michelia coriacea, a critically endangered tree, has a restricted and fragmented distribution in Southeast Yunnan Province, China. The genetic diversity, genetic structure and gene flow in the three extant populations of this species were detected by 10 inter-simple sequence repeat (ISSR) markers and 11 simple sequence repeat (SSR) markers. Examination of genetic diversity revealed that the species maintained a relatively high level of genetic diversity at the species level (percentage of polymorphic bands) PPB = 96.36% from ISSRs; PPL (percentage of polymorphic loci) = 95.56% from SSRs, despite several fragmental populations. Low levels of genetic differentiation among the populations of M. coriacea were detected by Nei’s Gst = 0.187 for ISSR and Wright’s Fst = 0.090 for SSR markers, which is further confirmed by Bayesian model-based STRUCTURE and PCoA analysis that could not reveal a clear separation between populations, although YKP was differentiated to other two populations by ISSR markers. Meanwhile, AMOVA analysis also indicated that 22.84% and 13.90% of genetic variation existed among populations for ISSRs and SSRs, respectively. The high level of genetic diversity, low genetic differentiation, and the population, structure imply that the fragmented habitat and the isolated population of M. coriacea may be due to recent over-exploitation. Conservation and management of M. coriacea should concentrate on maintaining the high level of genetic variability through both in and ex-situ conservation actions.

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“…Extracted DNA was dissolved in 50 μL Tris-EDTA (TE) buffer and stored at −20°C for amplification via polymerase chain reaction (PCR). Through preliminary screening using plastid and nuclear primers, 3 universal cpDNA intergenic spacers (matK, psbA-trnH, and TrnL-trnF) and 10 microsatellite markers were selected from 32 recently developed nuclear microsatellites in Magnoliaceae (Zhao et al, 2009 ; Sun et al, 2010 ; Li et al, 2012 ) on the basis of their clarity and reproducibility.…”

Section: Methodsmentioning

confidence: 99%

Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers

Zhang

Shen

et al. 2017

Front. Plant Sci.

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Michelia yunnanensis Franch., is a traditional ornamental, aromatic, and medicinal shrub that endemic to Yunnan Province in southwest China. Although the species has a large distribution pattern and is abundant in Yunnan Province, the populations are dramatically declining because of overexploitation and habitat destruction. Studies on the genetic variation and demography of endemic species are necessary to develop effective conservation and management strategies. To generate such knowledge, we used 3 pairs of universal cpDNA markers and 10 pairs of microsatellite markers to assess the genetic diversity, genetic structure, and demographic history of 7 M. yunnanensis populations. We calculated a total of 88 alleles for 10 polymorphic loci and 10 haplotypes for a combined 2,089 bp of cpDNA. M. yunnanensis populations showed high genetic diversity (Ho = 0.551 for nuclear markers and Hd = 0.471 for cpDNA markers) and low genetic differentiation (FST = 0.058). Geographical structure was not found among M. yunnanensis populations. Genetic distance and geographic distance were not correlated (P > 0.05), which indicated that geographic isolation is not the primary cause of the low genetic differentiation of M. yunnanensis. Additionally, M. yunnanensis populations contracted ~20,000–30,000 years ago, and no recent expansion occurred in current populations. Results indicated that the high genetic diversity of the species and within its populations holds promise for effective genetic resource management and sustainable utilization. Thus, we suggest that the conservation and management of M. yunnanensis should address exotic overexploitation and habitat destruction.

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Isolation and characterization of 12 microsatellite loci for Michelia coriacea (Magnoliaceae), a critically endangered endemic to Southeast Yunnan, China

Cited by 9 publications

References 5 publications

Isolation and characterization of microsatellite loci in Tsoongiodendron odorum (Magnoliaceae)

Isolation and characterization of microsatellite loci in Tsoongiodendron odorum (Magnoliaceae)

High Genetic Diversity and Low Differentiation of Michelia coriacea (Magnoliaceae), a Critically Endangered Endemic in Southeast Yunnan, China

Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers

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