Isolation and characterization of a newly identified Haemophilus species from cats: "Haemophilus felis"

Inzana, Thomas J.; Johnson, John L.; Möller, Kristian; Kilian, M

doi:10.1128/jcm.30.8.2108-2112.1992

Cited by 22 publications

(3 citation statements)

References 11 publications

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“…A wide variety of bacterial genera have the genes to make proteins to synthesize a glycogen polymer, and the genera with the greatest amino acid sequence identity to those in P. multocida are other members of the family Pasteurellaceae . Of interest is that some of these proteins are closest in identity to those from Haemophilus felis , which, like P. multocida , lives in the upper respiratory tracts of cats ( 64 ). Surface expression of CPS may also inhibit biofilm formation by blocking cell surface adhesins ( 59 ).…”

Section: Discussionmentioning

confidence: 99%

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Petruzzi

Briggs

Tatum

et al. 2017

mBio

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Pasteurella multocida is an important multihost animal and zoonotic pathogen that is capable of causing respiratory and multisystemic diseases, bacteremia, and bite wound infections. The glycosaminoglycan capsule of P. multocida is an essential virulence factor that protects the bacterium from host defenses. However, chronic infections (such as swine atrophic rhinitis and the carrier state in birds and other animals) may be associated with biofilm formation, which has not been characterized in P. multocida. Biofilm formation by clinical isolates was inversely related to capsule production and was confirmed with capsule-deficient mutants of highly encapsulated strains. Capsule-deficient mutants formed biofilms with a larger biomass that was thicker and smoother than the biofilm of encapsulated strains. Passage of a highly encapsulated, poor-biofilm-forming strain under conditions that favored biofilm formation resulted in the production of less capsular polysaccharide and a more robust biofilm, as did addition of hyaluronidase to the growth medium of all of the strains tested. The matrix material of the biofilm was composed predominately of a glycogen exopolysaccharide (EPS), as determined by gas chromatography-mass spectrometry, nuclear magnetic resonance, and enzymatic digestion. However, a putative glycogen synthesis locus was not differentially regulated when the bacteria were grown as a biofilm or planktonically, as determined by quantitative reverse transcriptase PCR. Therefore, the negatively charged capsule may interfere with biofilm formation by blocking adherence to a surface or by preventing the EPS matrix from encasing large numbers of bacterial cells. This is the first detailed description of biofilm formation and a glycogen EPS by P. multocida.

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Section: Discussionmentioning

confidence: 99%

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Petruzzi

Briggs

Tatum

et al. 2017

mBio

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“…Cell motility was detected under dark field microscope after cultivation on Haemophilus chocolate 2 agar at 35 °C under 5 % CO 2 atmosphere for 2 days. The requirement for V factor was assayed by growth around commercial discs supplemented with NAD on BHI agar, and growth on BHI agar with or without NAD supplement in the medium [18, 19]. The requirement for X factor was determined by the porphyrin test [20, 21] and by the growth around commercial discs supplemented with X and V+X factors (Liofilchem) on BHI agar.…”

Section: Full-textmentioning

confidence: 99%

Haemophilus seminalis sp. nov., isolated from human semen

Zheng

Liu

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Two Haemophilus-like isolates with similar biochemical characteristics, designated strains SZY H1 T and SZY H2, were isolated from human semen specimens. Cells were Gram-negative, non-motile, non-acid-fast, pleomorphic rods or coccobacilli. The major fatty acids (>10 %) were C 16 : 0 , C 14 : 0 , iso-C 16 : 0 and/or C 14 : 0 3-OH and C 16 : 1 ω6c and/or C 16 : 1 ω7c. The polar lipids were determined to be phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminophospholipid, two unidentified polar lipids and four unidentified aminolipids. The major polyamine was found to be cadaverine. The near-full-length (1462 nt) 16S rRNA gene sequences analysis showed the two isolates were nearly identical (>99.8 %), and closely matched Haemophilus haemolyticus ATCC 33390 T with 98.9-99.1 % sequence similarities. Phylogenetic analysis based on 16S rRNA gene sequences and concatenation of 30 protein markers also revealed that the isolates clustered together with H. haemolyticus ATCC 33390 T , and formed a distinct lineage well separated from the other members of the genus Haemophilus. Further, the average nucleotide identity values between the two isolates and their related species were below the established cutoff values for species delineation (95 %). Based on these findings, the two isolates are considered to represent a new species of the genus Haemophilus, for which name Haemophilus seminalis sp. nov. is proposed. The type strain is SZY H1 T (=NBRC 113782 T =CGMCC 1.17137 T).

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“…Species obtained from dogs and cats include Pasteurella multocida, Pasteurella dagmatis, Pasteurella stomatis and Pasteurella oralis. Two additional species are more restricted in their host-specificity, Pasteurella canis with dogs and [Haemophilus] felis with cats (Mutters et al 1985;Inzana et al 1992;Christensen et al 2012). In humans the aforementioned species may cause wound infections inflicted by dog-or cat bites/scratches (Abrahamian and Goldstein 2011).…”

Section: Introductionmentioning

confidence: 99%

Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds

Korczak

Bisgaard

Kuhnert

2014

Antonie van Leeuwenhoek

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Polyphasic analysis was done on 24 strains of Bisgaard taxon 16 from five European countries and mainly isolated from dogs and human dog-bite wounds. The isolates represented a phenotypically and genetically homogenous group within the family Pasteurellaceae. Their phenotypic profile was similar to members of the genus Pasteurella. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry clearly identified taxon 16 and separated it from all other genera of Pasteurellaceae showing a characteristic peak combination. Taxon 16 can be further separated and identified by a RecN protein signature sequence detectable by a specific PCR. In all phylogenetic analyses based on 16S rRNA, rpoB, infB and recN genes, taxon 16 formed a monophyletic branch with intraspecies sequence similarity of at least 99.1, 90.8, 96.8 and 97.2 %, respectively. Taxon 16 showed closest genetic relationship with Bibersteinia trehalosi as to the 16S rRNA gene (95.9 %), the rpoB (89.8 %) and the recN (74.4 %), and with Actinobacillus lignieresii for infB (84.9 %). Predicted genome similarity values based on the recN gene sequences between taxon 16 isolates and the type strains of known genera of Pasteurellaceae were below the genus level. Major whole cell fatty acids for the strain HPA 21(T) are C14:0, C16:0, C18:0 and C16:1 ω7c/C15:0 iso 2OH. Major respiratory quinones are menaquinone-8, ubiquinone-8 and demethylmenaquinone-8. We propose to classify these organisms as a novel genus and species within the family of Pasteurellaceae named Frederiksenia canicola gen. nov., sp. nov. The type strain is HPA 21(T) (= CCUG 62410(T) = DSM 25797(T)).

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Isolation and characterization of a newly identified Haemophilus species from cats: "Haemophilus felis"

Cited by 22 publications

References 11 publications

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Haemophilus seminalis sp. nov., isolated from human semen

Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds

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