Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds

Abstract

Polyphasic analysis was done on 24 strains of Bisgaard taxon 16 from five European countries and mainly isolated from dogs and human dog-bite wounds. The isolates represented a phenotypically and genetically homogenous group within the family Pasteurellaceae. Their phenotypic profile was similar to members of the genus Pasteurella. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry clearly identified taxon 16 and separated it from all other genera of Pasteurellaceae showing a characteristic peak combination. Taxon 16 can be further separated and identified by a RecN protein signature sequence detectable by a specific PCR. In all phylogenetic analyses based on 16S rRNA, rpoB, infB and recN genes, taxon 16 formed a monophyletic branch with intraspecies sequence similarity of at least 99.1, 90.8, 96.8 and 97.2 %, respectively. Taxon 16 showed closest genetic relationship with Bibersteinia trehalosi as to the 16S rRNA gene (95.9 %), the rpoB (89.8 %) and the recN (74.4 %), and with Actinobacillus lignieresii for infB (84.9 %). Predicted genome similarity values based on the recN gene sequences between taxon 16 isolates and the type strains of known genera of Pasteurellaceae were below the genus level. Major whole cell fatty acids for the strain HPA 21(T) are C14:0, C16:0, C18:0 and C16:1 ω7c/C15:0 iso 2OH. Major respiratory quinones are menaquinone-8, ubiquinone-8 and demethylmenaquinone-8. We propose to classify these organisms as a novel genus and species within the family of Pasteurellaceae named Frederiksenia canicola gen. nov., sp. nov. The type strain is HPA 21(T) (= CCUG 62410(T) = DSM 25797(T)).

Pasteurellaceae bacteria from the oral cavity of Tasmanian devils (Sarcophilus Harrisii ) show high minimum inhibitory concentration values towards aminoglycosides and clindamycin

“…This study appears to be the first to report MIC values of Pasteurellaceae isolated from Tasmanian devils. Since many of the strains in this study represent relatively recently described species, breakpoints have not yet been established for these bacteria (Korczak et al 2014;Brix et al 2015). For this reason it cannot be affirmed whether the isolates are sensitive or resistant, but by assessing whether the MIC values fit into the low, middle or high end of the concentration range, respectively, their susceptibility status can, at least partly, be predicted.…”

“…Since many of the strains in this study represent relatively recently described species, breakpoints have not yet been established for these bacteria (Korczak et al . ; Brix et al . ).…”

Interspecies dynamics among bacteria associated with canine periodontal disease

“…The Fr. canicola HPA 21 canine isolate was a gift from P. Kuhnert (University of Bern, Switzerland). Po.…”

Uruburuella testudinis sp. nov., isolated from tortoise (Testudo)

“…To get a clearer picture of the phylogenetic relationships and taxonomic positions partial sequences of 16S rRNA and rpoB genes for all 11 isolates as well as U. suis CCUG 47806 T and N. canis CCUG 56775 T were generated as described previously (Korczak et al , 2004; Kuhnert et al , 2002). The partial 16S rRNA and rpoB gene fragments were amplified by multiplex PCR (Korczak et al , 2014). Purified products were sequenced using a BigDye Terminator 3.1 cycle sequencing kit (Applied Biosystems) and run on an ABI 3130×l genetic analyser (Applied Biosystems).…”