Isolation and characterization of a cDNA encoding a sar-like monomeric GTP-binding protein in Avena fatua L.

Cranston, Harwood J.; Johnson, Russell R.; Chaverra, Marta; Dyer, William E.

doi:10.1016/s0168-9452(99)00073-4

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…Different types of proteins and genes have been identified and associated with changes in seed dormancy by comparing mRNA and protein profiles of dormant and nondormant genotypes or dormant seeds before and after stratification (Cranston et al 1999;Kawakami et al 1992). Several proteins showed changes in abundance in response to the incubation treatments.…”

Section: Intraspecific Physiological Differences In Seed Dormancy Regmentioning

confidence: 99%

Germination and proteome analyses reveal intraspecific variation in seed dormancy regulation in common waterhemp (Amaranthus tuberculatus)

León¹,

Bassham

Owen

2006

Weed sci.

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Common waterhemp is an obligate outcrosser that has high genetic variability. However, under selection pressure, this weed shows population differentiation for adaptive traits. Intraspecific variation for herbicide resistance has been studied, but no studies have been conducted to determine the existence of variation for other adaptive traits that could influence weed management. The objective of this study was to examine the existence of different seed dormancy regulatory mechanisms in common waterhemp. Seed dormancy regulation, in response to different temperature and moisture regimes, was studied through germination experiments and proteome analysis using two common waterhemp biotypes (Ames and Everly) collected from agricultural fields in Iowa, and one biotype (Ohio) collected from a pristine area in Ohio. Without stratification, germination percentage among the different biotypes was 9, 29 and 88% for Ames, Everly, and Ohio respectively. The germination rate of seeds from Ames was dramatically increased after incubation at either 4 or 25 C under wet conditions, whereas germination of seeds from Everly was only increased at 25 C under wet conditions. The Ohio biotype showed no change in germination response to any of the incubation treatments. Germination studies indicated that the rate of seed dormancy alleviation differed between biotypes. Seed protein profiles obtained from the three biotypes differed in protein abundance, number, and type. A putative small heat-shock protein (sHSP) of 17.6 kDa and isoelectric point (pI) 6.1 increased whereas a putative glyceraldehyde-3-phosphate dehydrogenase (G3PDH) of 30.9 kDa and pI 6.4 decreased in abundance in the Ames biotype as seed dormancy was reduced in response to incubation at 4 C and wet conditions. These two proteins did not change in the Everly and Ohio biotypes, suggesting that these proteins changed their abundance in response to seed dormancy alleviation. The results of this study suggest that differences in seed dormancy levels between the biotypes were due to different physiological regulatory mechanisms.

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Section: Intraspecific Physiological Differences In Seed Dormancy Regmentioning

confidence: 99%

Germination and proteome analyses reveal intraspecific variation in seed dormancy regulation in common waterhemp (Amaranthus tuberculatus)

León¹,

Bassham

Owen

2006

Weed sci.

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“…Different types of proteins and genes have been identified and associated with changes in seed dormancy by comparing mRNA and protein profiles of dormant and nondormant genotypes or dormant seeds before and after stratification (Cranston et al 1999;. Several proteins showed changes in abundance in response to the incubation treatments.…”

Section: Intra-specific Physiological Differences In Seed Dormancy Rementioning

confidence: 99%

Genetic and physiological characterization of seed dormancy regulation in common waterhemp [Amaranthus tuberculatus (Moq) Sauer]

Leon-Gonzalez

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Molecular analysis of ARF1 expression profiles during development of physic nut (Jatropha curcas L.)

et al. 2010

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A cDNA clone designated arf1 was isolated from a physic nut (Jatropha curcas L.) endosperm cDNA library which encodes a small GTP-binding protein and has significant homology to ADP-ribosylation factors (ARF) in plants, animals and microbes. The cDNA contains an open reading frame that encodes a polypeptide of 181 amino acids with a calculated molecular mass of 20.7 kDa. The deduced amino acid sequence showed high homology to known ARFs from other organisms. The products of the arf1 obtained by overexpression in E. coli revealed the specific binding activity toward GTP. The expression of arf1 was observed in flowers, roots, stems and leaves as analyzed by RT-PCR, and its transcriptional level was highest in flowers. In particular, the accumulation of arf1 transcripts was different under various environmental stresses in seedlings. The results suggest that arf1 plays distinct physiological roles in Jatropha curcas cells.

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Isolation and characterization of a cDNA encoding a sar-like monomeric GTP-binding protein in Avena fatua L.

Cited by 8 publications

References 23 publications

Germination and proteome analyses reveal intraspecific variation in seed dormancy regulation in common waterhemp (Amaranthus tuberculatus)

Germination and proteome analyses reveal intraspecific variation in seed dormancy regulation in common waterhemp (Amaranthus tuberculatus)

Genetic and physiological characterization of seed dormancy regulation in common waterhemp [Amaranthus tuberculatus (Moq) Sauer]

Molecular analysis of ARF1 expression profiles during development of physic nut (Jatropha curcas L.)

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