Isolation and characterization of a novel thermo-solvent-stable lipase from Pseudomonas brenneri and its application in biodiesel synthesis

Abstract

Pseudomonads are one of the most studied species of bacteria as they display remarkable metabolic and physiological versatility. This enables them to colonize a wide variety of terrestrial and aquatic habitats, generating biotechnologically interesting enzymes. Here, the partial purification and characterization of a novel, extracellularly-produced, lipase from Pseudomonas brenneri is described. The partially purified lipase was active over a broad pH range (5.0-9.0) and was stable at 70 °C for 45 min. The lipase displayed significant stability, and in some cases activation, in the presence of organic solvents with log P≥2.0. Such stability characteristics indicated that this lipase could potentially be useful as a biocatalyst for biodiesel production. This was subsequently demonstrated through the facile production of Fatty Acid Methyl Esters in the presence of olive oil and methanol. Possible applications for this novel, stable lipase include the bioremediation of oil in the environment.

Listeria monocytogenes is a solvent tolerant organism secreting a solvent stable lipase: potential biotechnological applications

“…BIM B-86, D1 as a Sphingomonas sp., D5 as Listeria monocytogenes , H1 as Pseudomonas reinekei and H3 as Pseudomonas brenneri. Studies of the solvent stable enzymes from P. brenneri and P. reinekei have previously been reported (Priyanka et al 2019a , b , 2020 ). The finding that L. monocytogenes was among the solvent tolerant organisms identified by the screening process was somewhat surprising since no reference to this property of Listeria was found in the literature.…”

Plant Growth Promoting Endophytic Bacteria Bacillus australimaris BLR41 and Enterobacter kobei BLR45 Enhance the Growth of Medicinal Plant Barleria lupulina Lindl.

“…12 Tween 20 peptone agar was used to test lipase activity, while glucose yeast peptone agar with gelatin was used to measure protease activity. 13 The catalase test was carried out by putting a freshly developed (24-hour-old) bacterial culture on (3 %) H2O2. 14 Gram staining was performed with 48 h grown culture and shape of the bacterial cell was observed.…”

Produção Microbiológica De Enzimas: Uma Revisão/Microbiological Production of Enzymes: A Review

“…Portanto, para evitar a precipitação e permitir o uso do dialisado após precipitação alcalina para cromatografia, o pH do dialisado foi reduzido para 8,5. Após a o processo de purificação, um resultado de Brazilian Journal of Development, Curitiba, v.7, n.1, p.9232-9254 jan. 2021 atividade específica de 3,18 IU/mg da lipase foi alcançado, com um rendimento geral de 56,89% (Priyanka et al, 2020).…”