Isolation and Characterization of a Novel Lipase from a Metagenomic Library of Tidal Flat Sediments: Evidence for a New Family of Bacterial Lipases

Lee, Mi-Hwa; Lee, Choong-Hwan; Oh, Tae-Kwang; Song, Jae Kwang; Yoon, Jung‐Hoon

doi:10.1128/aem.01157-06

Cited by 179 publications

(134 citation statements)

References 25 publications

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“…Due to the limitations of culturing, metagenomic screening has become a powerful tool to exploit the biocatalytic potential of microbial communities for the discovery of novel biocatalysts (13,20). Our previous investigation has demonstrated the existence of many novel uncultured microorganisms in black liquor sediment.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Metagenome-Derived 6-Phospho-β-Glucosidase from Black Liquor Sediment

Yang

Niu

et al. 2013

Appl Environ Microbiol

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The enzyme 6-phospho-␤-glucosidase is an important member of the glycoside hydrolase family 1 (GH1). However, its catalytic mechanisms, especially the key residues determining substrate specificity and affinity, are poorly understood. A metagenomederived gene sequence, encoding a novel 6-phospho-␤-glucosidase designated Pbgl25-217, was isolated and characterized. The optimal conditions for enzymatic activity were 37°C and pH 7; Ca 2؉ , Mg 2؉ , and Mn 2؉ stabilized the activity of Pbgl25-217, whereas Ni 2؉ , Fe 2؉ , Zn 2؉ , Cu 2؉ , and Fe 3؉ inhibited its activity. The K m and V max of Pbgl25-217 were 4.8 mM and 1,987.0 U mg ؊1 , respectively. Seven conserved residues were recognized by multiple alignments and were tested by site-directed mutagenesis for their functions in substrate recognition and catalytic reaction. The results suggest that residues S427, Lys435, and Tyr437 act as "gatekeepers" in a phosphate-binding loop and play important roles in phosphate recognition. This functional identification may provide insights into the specificity of 6-phospho-␤-glycosidases in GH1 and be useful for designing further directed evolution.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Metagenome-Derived 6-Phospho-β-Glucosidase from Black Liquor Sediment

Yang

Niu

et al. 2013

Appl Environ Microbiol

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“…This approach also overcomes the problem of cultivation of microbes as majority of the micro organisms are not to cultivation (Handelsman et al, 1998). There were so many recent reports proving the novelty of this approach in the identification of true lipases (Jiang et al, 2006;Lee et al, 2006;Elend et al, 2007;Jeon et al, 2009;Liu et al, 2009;Meilleur et al, 2009;Glogauer et al, 2011).…”

Section: Molecular Methods For Screening Of Lipasesmentioning

confidence: 99%

A Short Review on Various Screening Methods to Isolate Potential Lipase Producers: Lipases-the Present and Future Enzymes of Biotech Industry

Lanka¹,

Latha²

2015

International J. of Biological Chemistry

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The increased demand for microbial originated Industrial enzymes especially lipases which have received least priority till last one and half decades and gained lots of importance in recent days owing to their applications in wide variety of fields such as food, dairy, pharmaceutical, detergent, textile, oleo-chemical, perfume and cosmetic industries etc., has lead to the identification of novel enzymes from new sources with unique properties. The present review mainly focuses on various currently available screening methods for identification of lipase producers.

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“…In contrast to a purely sequence-based screening of 30 metagenomic libraries, screening or selecting for function is, theoretically, more likely to discover completely novel enzymes given that prior genetic information is not important for the recovery of gene targets. Examples of recovering targets based on function include discoveries of lipases from an Atlantic rainforest soil [1] and intertidal flat sediments [2,3], and an esterase from South China Sea sediments [4], all of which were assigned to novel enzyme families.…”

Section: Introduction To Metagenomicsmentioning

confidence: 99%

Targeted metagenomics of active microbial populations with stable-isotope probing

Coyotzi

Pratscher

Murrell

et al. 2016

Current Opinion in Biotechnology

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SummaryThe ability to explore microbial diversity and function has been enhanced by novel experimental and computational tools. The incorporation of stable isotopes into microbial 15 biomass enables the recovery of labeled nucleic acids from active microorganisms, despite their initial abundance and culturability. Combining stable-isotope probing (SIP) with metagenomics provides access to genomes from microorganisms involved in metabolic processes of interest.Studies using metagenomic analysis on DNA obtained from DNA-SIP incubations can be ideal for the recovery of novel enzymes for biotechnology applications, including biodegradation, 20 biotransformation, and biosynthesis. This chapter introduces metagenomic and DNA-SIP methodologies, highlights biotechnology-focused studies that combine these approaches, and provides perspectives on future uses of these methods as analysis tools for applied and environmental microbiology.

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Isolation and Characterization of a Novel Lipase from a Metagenomic Library of Tidal Flat Sediments: Evidence for a New Family of Bacterial Lipases

Cited by 179 publications

References 25 publications

Characterization of a Novel Metagenome-Derived 6-Phospho-β-Glucosidase from Black Liquor Sediment

Characterization of a Novel Metagenome-Derived 6-Phospho-β-Glucosidase from Black Liquor Sediment

A Short Review on Various Screening Methods to Isolate Potential Lipase Producers: Lipases-the Present and Future Enzymes of Biotech Industry

Targeted metagenomics of active microbial populations with stable-isotope probing

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