Isolation and characterization of a rhabdovirus from co-infection of two viruses in mandarin fish

Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF

“…Also, in view of its high conservation (Le Mercier et al, 1997), the phylogenetic relationship analysis of L protein sequences between SCRV and other rhabdoviruses was conducted, and showed that SCRV clusters with SVCV and is most closely related to Vesiculoviruses. This result is also consistent with our previously report (Tao et al, 2007).…”

“…The procedures of virus propagation and purification were according to previous reports (Zhang et al, 2006;Tao et al, 2007). The virus pellet was resuspended in TE buffer (10 mM Tris-Cl, 1 mM EDTA, pH 7.4) and stored at −20 • C until use.…”

“…Rhabdoviruses are usually nonsegmented, single-strand and negative-sense RNA viruses except the Orchid fleck virus (Tao et al, 2007). Multiple sequence alignment has shown that the N protein of SCRV shares 6.9-41.5% identity with other rhabdoviruses and phylogenetic analysis has indicated SCRV was most closely related to Vesiculoviruses.…”

“…It had been confirmed that SCRV can coinfect with Siniperca chuatsi spherical virus (SCSV) in cultured fish cells and cause cytopathic effect (CPE) (Zhang and Li, 1999a,b;Zhang et al, 2005). Recently, SCRV was separated from the coinfecting viruses by plaque isolation and the physico-chemical characteristics were determined (Tao et al, 2007).…”

Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro

“…SCRV is an enveloped single-stranded RNA (ssRNA) virus with a typical bullet shape [28,29]. MsReV is a non-enveloped double-stranded RNA (dsRNA) virus, infection by which leads to a typical cytopathic effect (CPE) characterized by cellecell fusion and syncytium formation [30].…”