Isolation and characterization of a novel Staphylococcus aureus bacteriophage, ϕMR25, and its therapeutic potential

Hoshiba, Hiroshi; Uchiyama, Jumpei; Kato, Shin‐ichiro; Ujihara, Takako; Muraoka, Asako; Daibata, Masanori; Wakiguchi, Hiroshi; Matsuzaki, Shigenobu

doi:10.1007/s00705-010-0623-2

Cited by 28 publications

(13 citation statements)

References 27 publications

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“…The transduction activities of other phages are also of interest in staphylococci. Thus, we examined the generalized transduction activity and host range using three types of staphylococcal phages: siphophages fMR25 and 80, podophage S13 0 and myophage S25-3 Hoshiba et al, 2010;Takemura-Uchiyama et al, 2013) (Supplementary Table S2). Phages 80 and fMR25 were S. aureusspecific phages that exhibited transduction activities in S. aureus (Supplementary Table S1 and Supplementary Figure S4).…”

Section: Generalized Transduction In Staphylococcus Sppmentioning

confidence: 99%

Intragenus generalized transduction in Staphylococcus spp. by a novel giant phage

et al. 2014

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Bacteriophage (phage)-mediated generalized transduction is expected to contribute to the emergence of drug-resistant staphylococcal clones in various environments. In this study, novel phage S6 was isolated from sewage and used to test generalized transduction in human-and animal-derived staphylococci. Phage S6 was a novel type of giant myophage, which possessed a DNA genome that contained uracil instead of thymine, and it could infect all of the tested staphylococcal species. The phage S6 appeared to be similar to the transducing phage PBS1, which infects Bacillus spp. Moreover, phage S6 facilitated the transduction of a plasmid in Staphylococcus aureus and from S. aureus to non-aureus staphylococcal species, as well as vice versa. Transduction of methicillin resistance also occurred in S. aureus. This is the first report of successful intragenus generalized transduction among staphylococci.

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Section: Generalized Transduction In Staphylococcus Sppmentioning

confidence: 99%

Intragenus generalized transduction in Staphylococcus spp. by a novel giant phage

et al. 2014

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“…Specifically, several assays have been performed against S. aureus bacteremia. The intraperitoneal administration of phages phiMR11 and phiMR25 rescued mice inoculated with a lethal dose of S. aureus (18,30). Moreover, in a rabbit model of wound infection caused by S. aureus, phages prevented abscess formation (44).…”

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Enhanced Staphylolytic Activity of the Staphylococcus aureus Bacteriophage vB_SauS-phiIPLA88 HydH5 Virion-Associated Peptidoglycan Hydrolase: Fusions, Deletions, and Synergy with LysH5

Rodríguez-Rubio

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Rodrı́guez

et al. 2012

Appl Environ Microbiol

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ABSTRACTVirion-associated peptidoglycan hydrolases have potential as antimicrobial agents due to their ability to lyse Gram-positive bacteria on contact. In this work, our aim was to improve the lytic activity of HydH5, a virion-associated peptidoglycan hydrolase from theStaphylococcus aureusbacteriophage vB_SauS-phiIPLA88. Full-length HydH5 and two truncated derivatives containing only the CHAP (cysteine, histidine-dependent amidohydrolase/peptidase) domain exhibited high lytic activity against liveS. aureuscells. In addition, three different fusion proteins were created between lysostaphin and HydH5, each of which showed higher staphylolytic activity than the parental enzyme or its deletion construct. Both parental and fusion proteins lysedS. aureuscells in zymograms and plate lysis and turbidity reduction assays. In plate lysis assays, HydH5 and its derivative fusions lysed bovine and humanS. aureusstrains, the methicillin-resistantS. aureus(MRSA) strain N315, and humanStaphylococcus epidermidisstrains. Several nonstaphylococcal bacteria were not affected. HydH5 and its derivative fusion proteins displayed antimicrobial synergy with the endolysin LysH5in vitro, suggesting that the two enzymes have distinct cut sites and, thus, may be more efficient in combination for the elimination of staphylococcal infections.

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“…Numerous S. aureus phages have been isolated and characterized at the molecular level (2,6,22,32,33,36,37,39,42,44,46,47). They all belong to the order Caudovirales, with the most abundant being Siphoviridae phages, typically composed of a long noncontractile tail and an icosahedral head filled with doublestranded DNA.…”

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Characterization of Novel Phages Isolated in Coagulase-Negative Staphylococci Reveals Evolutionary Relationships with Staphylococcus aureus Phages

et al. 2012

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Despite increasing interest in coagulase-negative staphylococci (CoNS), little information is available about their bacteriophages. We isolated and sequenced three novel temperate Siphoviridae phages (StB12, StB27, and StB20) from the CoNS Staphylococcus hominis and S. capitis species. The genome sizes are around 40 kb, and open reading frames (ORFs) are arranged in functional modules encoding lysogeny, DNA metabolism, morphology, and cell lysis. Bioinformatics analysis allowed us to assign a potential function to half of the predicted proteins. Structural elements were further identified by proteomic analysis of phage particles, and DNA-packaging mechanisms were determined. Interestingly, the three phages show identical integration sites within their host genomes. In addition to this experimental characterization, we propose a novel classification based on the analysis of 85 phage and prophage genomes, including 15 originating from CoNS. Our analysis established 9 distinct clusters and revealed close relationships between S. aureus and CoNS phages. Genes involved in DNA metabolism and lysis and potentially in phage-host interaction appear to be widespread, while structural genes tend to be cluster specific. Our findings support the notion of a possible reciprocal exchange of genes between phages originating from S. aureus and CoNS, which may be of crucial importance for pathogenesis in staphylococci.

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Isolation and characterization of a novel Staphylococcus aureus bacteriophage, ϕMR25, and its therapeutic potential

Cited by 28 publications

References 27 publications

Intragenus generalized transduction in Staphylococcus spp. by a novel giant phage

Intragenus generalized transduction in Staphylococcus spp. by a novel giant phage

Enhanced Staphylolytic Activity of the Staphylococcus aureus Bacteriophage vB_SauS-phiIPLA88 HydH5 Virion-Associated Peptidoglycan Hydrolase: Fusions, Deletions, and Synergy with LysH5

Characterization of Novel Phages Isolated in Coagulase-Negative Staphylococci Reveals Evolutionary Relationships with Staphylococcus aureus Phages

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