Isolation and characterization of a novel thermostable lectin from the wild edible mushroom Agaricus arvensis

Abstract

A thermostable novel lectin with a molecular weight of 30.4 kDa was isolated from dried fruiting bodies of Agaricus arvensis. It was a dimer made up of two 15.2 kDa subunits. The lectin was unadsorbed on DEAE-cellulose in 10 mM phosphate buffer (pH 7.5), subsequently adsorbed on CM-cellulose in 10 mM NaAc buffer (pH 4.6) and then on SP-Sepharose in 10 mM NaAc buffer (pH 4.6), and finally purified by fast protein liquid chromatography-gel filtration on Superdex 75. The hemagglutinating activity of the lectin was stable at temperatures up to 90 °C. The activity was preserved in concentrations of NaOH solution up to 50 mM, but was sensitive to HCl and declined to 12.5% in 12.5 mM HCl. The activity was unaffected by Ca(2+), Mn(2+), Zn(2+) and Mg(2+) ions, but was activated by Al(3+) and Fe(3+) ions. Among the carbohydrates tested, only inulin could inhibit the hemagglutinating activity of the lectin. It did not exhibit anti-HIV reverse transcriptase activity. Proliferation of HepG2 and MCF7 tumor cells was inhibited by the lectin with an IC(50) of 1.64 and 0.82 μM, respectively. The lectin was devoid of antifungal activity. The lectin has a remarkable thermostablity and a unique N-terminal amino acid sequence, TYAVLNFVYG. The present report is the first report on a lectin from wild mushroom Agaricus arvensis.

A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic MushroomBoletus speciosus

“…The cultures were then harvested by using a cell harvester. The incorporated radioactivity was determined by liquid scintillation counting [14]. …”

“…A fixed amount (4–6 ng) of recombinant HIV-1 RT was used. The inhibitory activity of the hemagglutinin was calculated as percentage inhibition as compared to a control without the protein [14]. …”

Mushroom Lectins: Specificity, Structure and Bioactivity Relevant to Human Disease

“…Mushrooms species where multiple lectins have been identified, and those that are further discussed and listed in Table 2 , Table 3 and Table 4 , the published lectin abbreviations have been given in Table 1 . As illustrated in Table 1 , mushroom lectins have been identified with varying sugar specificities, from lectins that only bind the polysaccharide inulin (e.g., lectins from Agaricus arvensis [ 48 ], Pholiota adiposa [ 133 ], Xerocomus spadiceus [ 161 ]) to lectins that bind lactose ( Agaricus bitorquis , Boletus subtomentosus , Coprinus atramentarius , Pleurotus spodoleucus [ 7 , 74 , 140 ]), galactose ( Boletus sataaus and Panus conchatus [ 40 , 73 , 129 ]), N -acetylgalactosamine ( Ciborinia camelliae , Inocybe fastigiata , Lactarius vellereus , Pleurotus serotinus [ 77 , 98 , 129 ]), and sialic acid ( Hericium erinaceus , Polyporus squamosus , Psathyrella velutina , Paecilomyes japonica , and Agrocybe cylindracea [ 62 ]).…”

Fungal Proteins with Antiproliferative and Anticancer Activities

“…Proliferation of HepG2 and MCF7 tumor cells was inhibited by a 30.4 kDa thermostable dimeric lectin from Agaricus arvensis with an IC 50 of 1.64 and 0.82 DM, respectively [34].…”