Isolation and Characterization of a Burkholderia sp. USM (JCM15050) Capable of Producing Polyhydroxyalkanoate (PHA) from Triglycerides, Fatty Acids and Glycerols

Chee, Jiun-Yee; Tan, Yifen; Samian, Mohammed Razip; Sudesh, Kumar

doi:10.1007/s10924-010-0204-1

Cited by 79 publications

(39 citation statements)

References 34 publications

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“…USM was identified to be capable of converting palm oil, fatty acids, and glycerol byproducts into P(3HB). 95 Zhu 88 used Burkholderia cepacia to grow on tall oil fatty acids, consisting of 52% oleic acid and 45% linoleic acid, for PHA production. The maximum CDW and P(3HB) yield reached 3.8 g/L and 50% of CDW, respectively, under these conditions.…”

Section: Production and Characterization Of Polyhydrox-yalkanoates Frmentioning

confidence: 99%

Bioplastics from waste glycerol derived from biodiesel industry

Zhu

Chiu

Nakas

et al. 2013

J of Applied Polymer Sci

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Polyhydroxyalkanoates (PHAs) are polyesters that can be biologically synthesized by many microorganisms and engineered plants that have been investigated by microbiologists, biochemists, polymer scientists, material engineers, and medical researchers for several decades. Research on microbial production of PHAs has been extensively focused on using pure carbon sources, such as sugars and fatty acids. Practical considerations of production costs of PHAs have resulted in research efforts to use alternative renewable and inexpensive feedstocks. One potential feedstock for the production of PHA polymers is the glycerol waste byproduct of biodiesel production. The major focus of this review is the production of PHA polymers from glycerol. A review of biosynthetic pathways for PHAs production from glycerol, current production of waste glycerol in biodiesel industry, physical and mechanical properties of PHAs, and applications of PHAs in the areas of packaging industry, implant materials, drug carrier, biofuels, are covered.

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Section: Production and Characterization Of Polyhydrox-yalkanoates Frmentioning

confidence: 99%

Bioplastics from waste glycerol derived from biodiesel industry

Zhu

Chiu

Nakas

et al. 2013

J of Applied Polymer Sci

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“…In our previous work, Burkholderia sp. USM (JCM15050) was found to possess the ability to utilize palm oil products for the synthesis of P(3HB) homopolymer (Chee et al. 2010b).…”

Section: Resultsmentioning

confidence: 99%

“…USM (JCM15050). As both CPKO and PKAO are rich in lauric acid (C12) and myristic acid (C14) (Chee et al. 2010b), these two fatty acids were tested separately as the sole carbon source to investigate their contribution to the synthesis of 3HHx monomer.…”

Section: Resultsmentioning

confidence: 99%

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Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products

Chee

Lau

Samian

et al. 2011

Journal of Applied Microbiology

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Aims: Burkholderia sp. USM (JCM15050) isolated from oil‐polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3‐hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3‐hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA) synthase gene of Aeromonas caviae (phaCAc) was transformed into this strain. Methods and Results: The resulting transformant incorporated approximately 1 ± 0·3 mol% of 3HHx in the polymer when crude palm kernel oil (CPKO) or palm kernel acid oil was used as the sole carbon source. In addition, when the transformed strain was cultivated in the mixtures of CPKO and sodium valerate, PHA containing 69 mol% 3HB, 30 mol% 3‐hydroxyvalerate and 1 mol% 3HHx monomers was produced. Batch feeding of carbon sources with 0·5% (v/v) CPKO at 0 h and 0·25% (w/v) sodium valerate at 36 h yielded 6 mol% of 3HHx monomer by controlled‐feeding strategies. Conclusions: Burkholderia sp. USM (JCM15050) has the metabolic pathways to supply both the short‐chain length (SCL) and medium‐chain length (MCL) PHA monomers. By transforming the strain with the Aer. caviae PHA synthase with broader substrate specificity, SCL‐MCL PHA was produced. Significance and Impact of the Study: This is the first study demonstrating the ability of transformant Burkholderia to produce P(3HB‐co‐3HHx) from a single carbon source.

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Polyhydroxyalkanoates

Zainab-L

Yean

Sudesh

2018

Kirk-Othmer Encyclopedia of Chemical Technology

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Plastic waste accumulation in the environment has become a global issue. The search for alternative materials motivated the research on polyhydroxyalkanoates (PHAs), a biopolymer having properties similar to polypropylene. PHAs are biodegradable, biocompatible, and environment‐friendly thermoplastics, and therefore, a potential substitute for some nonbiodegradable commodity plastics. The favorable plastic‐like properties of PHA give rise to applications in various areas. PHAs are expensive compared to conventional plastics, but improved PHA biosynthesis has reduced the cost significantly. Current research is focused on utilization of low cost substrates, development of simple and cost‐effective extraction methods, and use of mixed microbial cultures and recombinant microbial strains in order to achieve economically feasible production of PHAs.This article provides background on PHAs, their properties, and biosynthesis. Large‐scale production, commercialization, application, and sustainability considerations are presented and discussed. Interesting features of PHAs and their role from an industrial ecology perspective are highlighted.

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Isolation and Characterization of a Burkholderia sp. USM (JCM15050) Capable of Producing Polyhydroxyalkanoate (PHA) from Triglycerides, Fatty Acids and Glycerols

Cited by 79 publications

References 34 publications

Bioplastics from waste glycerol derived from biodiesel industry

Bioplastics from waste glycerol derived from biodiesel industry

Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products

Polyhydroxyalkanoates

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