Isolation and characterization of a novel selenate-reducing bacterium, Bacillus sp. SF-1

Fujita, Masanori; Ike, Michihiko; Nishimoto, Shintaro; Takahashi, Kazuaki W.; Kashiwa, Masami

doi:10.1016/s0922-338x(97)81130-0

Cited by 80 publications

(71 citation statements)

References 15 publications

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“…Evidence for dissimilatory reduction of SeO 4 2Ϫ was first reported by Oremland et al (39) and, finally, to elemental selenium. Subsequent studies showed that phylogenetically diverse groups of microorganisms are able to couple dissimilatory SeO 4 2Ϫ reduction with anaerobic growth (37,(40)(41)(42)(43)(44). Interestingly, SeO 4 2Ϫ -reducing bacteria not only are phylogenetically diverse (Fig.…”

Section: Selenate-respiring Bacteriamentioning

confidence: 99%

“…selenatarsenatis SF-1. B. selenatarsenatis SF-1 is a Grampositive bacterium that was isolated from the effluent sediments of a glass manufacturing plant by using lactate and SeO 4 2Ϫ as the electron donor and acceptor, respectively (40,87). The strain shows a stoichiometric relationship between cell growth, lactate consumption, and SeO 4 2Ϫ reduction.…”

Section: [3fe-4s] and [4fe-4s]) Clusters (81) Coordination Of One [3mentioning

confidence: 99%

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Ecology and Biotechnology of Selenium-Respiring Bacteria

Nancharaiah

2015

Microbiol Mol Biol Rev

361

232

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SUMMARY In nature, selenium is actively cycled between oxic and anoxic habitats, and this cycle plays an important role in carbon and nitrogen mineralization through bacterial anaerobic respiration. Selenium-respiring bacteria (SeRB) are found in geographically diverse, pristine or contaminated environments and play a pivotal role in the selenium cycle. Unlike its structural analogues oxygen and sulfur, the chalcogen selenium and its microbial cycling have received much less attention by the scientific community. This review focuses on microorganisms that use selenate and selenite as terminal electron acceptors, in parallel to the well-studied sulfate-reducing bacteria. It overviews the significant advancements made in recent years on the role of SeRB in the biological selenium cycle and their ecological role, phylogenetic characterization, and metabolism, as well as selenium biomineralization mechanisms and environmental biotechnological applications.

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Section: Selenate-respiring Bacteriamentioning

confidence: 99%

Section: [3fe-4s] and [4fe-4s]) Clusters (81) Coordination Of One [3mentioning

confidence: 99%

Ecology and Biotechnology of Selenium-Respiring Bacteria

Nancharaiah

2015

Microbiol Mol Biol Rev

361

232

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“…We previously isolated a selenate-reducing bacterium, strain SF-1, from the effluent sediment of a glass manufacturing plant (8) and identified it as a new species, Bacillus selenatarsenatis (32). Strain SF-1 is able to reduce selenate to selenite, arsenate to arsenite, and nitrate to nitrite, independently, by anaerobic respiration (13,30).…”

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confidence: 99%

Molecular Cloning and Characterization of the srdBCA Operon, Encoding the Respiratory Selenate Reductase Complex, from the Selenate-Reducing Bacterium Bacillus selenatarsenatis SF-1

et al. 2011

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Previously, we isolated a selenate-and arsenate-reducing bacterium, designated strain SF-1, from seleniumcontaminated sediment and identified it as a novel species, Bacillus selenatarsenatis. B. selenatarsenatis strain SF-1 independently reduces selenate to selenite, arsenate to arsenite, and nitrate to nitrite by anaerobic respiration. To identify the genes involved in selenate reduction, 17 selenate reduction-defective mutant strains were isolated from a mutant library generated by random insertion of transposon Tn916. Tn916 was inserted into the same genome position in eight mutants, and the representative strain SF-1AM4 did not reduce selenate but did reduce nitrate and arsenate to the same extent as the wild-type strain. The disrupted gene was located in an operon composed of three genes designated srdBCA, which were predicted to encode a putative oxidoreductase complex by the BLASTX program. The plasmid vector pGEMsrdBCA, containing the srdBCA operon with its own promoter, conferred the phenotype of selenate reduction in Escherichia coli DH5␣, although E. coli strains containing plasmids lacking any one or two of the open reading frames from srdBCA did not exhibit the selenate-reducing phenotype. Domain structure analysis of the deduced amino acid sequence revealed that SrdBCA had typical features of membrane-bound and molybdopterin-containing oxidoreductases. It was therefore proposed that the srdBCA operon encoded a respiratory selenate reductase complex. This is the first report of genes encoding selenate reductase in Gram-positive bacteria.Selenium is the 34th element on the periodic table and has chemical properties resembling those of sulfur. It is obtained in limited ways, such as a by-product of the electric smelting of copper, and is an important material used in photoelectric devices, photosensitive drums used in dry copying, semiconductors, and the colorization and decolorization of glasses. Biologically, selenium is an essential element used for the synthesis of selenocysteine contained in selenoproteins, such as mammalian glutathione peroxidase and bacterial formate dehydrogenase. However, exposure to higher concentrations of selenium is toxic, and therefore it is important to understand how environmental selenium is controlled. Selenium has several oxidation states in the environment, i.e., selenate (ϩVI), selenite (ϩIV), elemental selenium (0), selenide (ϪII), and organic selenium (ϪII), and it is known that prokaryotes play a major role in its oxidation and reduction (28).The molecular mechanisms of selenate reduction have been analyzed in some bacteria. The selenate reductase complex of Thauera selenatis has been intensively studied biochemically and genetically. The selenate reductase complex of T. selenatis is a soluble periplasmic protein (20) that consists of three subunits, i.e., a catalytic subunit containing a molybdenum cofactor [Mo(V)], a subunit containing iron-sulfur clusters (one [3Fe-4S] cluster and three [4Fe-4S] clusters), and a subunit containing heme b (6, 25). The genes e...

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“…These isolates include Bacillus sp. SF-1 from a selenium-polluted sediment (Fujita et al, 1997), Bacillus selenitireducens MLS10…”

mentioning

confidence: 99%

Ferrimonas futtsuensis sp. nov. and Ferrimonas kyonanensis sp. nov., selenate-reducing bacteria belonging to the Gammaproteobacteria isolated from Tokyo Bay

Nakagawa

Iino

Suzuki

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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Two novel mesophilic, facultatively anaerobic, selenate-reducing bacteria, designated strains FUT3661T and Asr22-7T, were isolated from a sediment sample and the alimentary tract of littleneck clams, respectively. Both sources of the samples were collected from the coast of Tokyo Bay, Japan. Cells were Gram-negative rods and motile by means of a polar flagellum. The strains reduced selenate to elemental selenium (Se0) and also reduced iron(III) oxyhydroxide, iron(III) citrate, arsenate, manganese(IV) oxide, elemental sulfur and oxygen and used lactate, pyruvate, yeast extract, tryptone and Casamino acids as electron donors and carbon sources. The strains contained both menaquinone (MK-7) and ubiquinones (Q-7 and Q-8) as isoprenoid quinones. The major fatty acids were C16 : 0 and C16 : 1 ω9c. The G+C content of the genomic DNA was 58.1 mol% for strain FUT3661T and 57.2 mol% for strain Asr22-7T. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were related to members of the genus Ferrimonas (<94.0 % similarities), although the two novel strains formed a separate lineage. 16S rRNA gene sequence similarity between strains FUT3661T and Asr22-7T was 96 %. On the basis of this polyphasic analysis, it was concluded that strains FUT3661T and Asr22-7T represent two novel species within the genus Ferrimonas, for which the names Ferrimonas futtsuensis sp. nov. (type strain FUT3661T=NBRC 101558T=DSM 18154T) and Ferrimonas kyonanensis sp. nov. (type strain Asr22-7T=NBRC 101286T=DSM 18153T) are proposed.

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Isolation and characterization of a novel selenate-reducing bacterium, Bacillus sp. SF-1

Cited by 80 publications

References 15 publications

Ecology and Biotechnology of Selenium-Respiring Bacteria

Ecology and Biotechnology of Selenium-Respiring Bacteria

Molecular Cloning and Characterization of the srdBCA Operon, Encoding the Respiratory Selenate Reductase Complex, from the Selenate-Reducing Bacterium Bacillus selenatarsenatis SF-1

Ferrimonas futtsuensis sp. nov. and Ferrimonas kyonanensis sp. nov., selenate-reducing bacteria belonging to the Gammaproteobacteria isolated from Tokyo Bay

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