Isolation and characterization of a calcium-sensitive alpha-actinin-like protein from human platelet cytoskeletons.

Rosenberg, S. L.; Stracher, Alfred; Burridge, Keith

doi:10.1016/s0021-9258(18)42994-8

Cited by 93 publications

(17 citation statements)

References 29 publications

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“…However, in case of pathological changes in the gland tissue, the barrier composed of endodermis, basal cell layer, and basement membrane may be destroyed, and PSA may leak into the lymphatic system and enter the circulation, increasing serum PSA levels [20,21]. It was reported that urinary tract infection, RDE, medical operations, and other factors can affect the serum content of PSA [22]. Therefore, PSA has some limitations in the early screening of PCa, especially with values in the "PSA gray zone".…”

Section: Discussionmentioning

confidence: 99%

The diagnostic value of a new formula combining age and prostate volume in prostate cancer

Yang¹,

Shan²,

Qiang³

et al. 2021

J. Mens. Health

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Background and objective: This study combined two clinical indicators (age and prostate volume (PV)) to generate age to PV (AVR) ratio, whose diagnostic value for prostate cancer (PCa) was examined based on prostate speciﬁc antigen (PSA) in the range of 4--20.0 ng/mL. Methods: The medical records of patients who underwent transrectal ultrasound-guided biopsy of the prostate in our hospital from June 2015 to June 2019 were examined retrospectively. According to the pathological results of the biopsy, the patients were divided into the PCa and benign prostatic hyperplasia (BPH) groups. Receiver operating characteristic (ROC) curves for TPSA, PSAD, PV, (F/T)PSA, AVR, and PSA-AV were plotted with SPSS 26.0 and GraphPad Prism 5.0, and areas under the ROC curves (AUROCs) were determined and compared by Delong test. A log-linear model was used to compare AVR and other parameters with similar high sensitivities, for speciﬁcity. Results: The AUROC for AVR was signiﬁcantly different from those of TPSA (p < 0.001), PV (p = 0.004),(F/T)PSA (p < 0.001), and PSA-AV (p = 0.006), and similar to that of PSAD (p = 0.064). With the same high sensitivity (90.0%), log-linear model analysis showed that the speciﬁcity of AVR was signiﬁcantly higher than those of TPSA and (F/T)PSA (p < 0.01), while there were no signiﬁcant differences among AVR and PSAD, PV and PSA-AV. Conclusion: With PSA in the range of 4--20.0 ng/mL, AVR may be useful in sparing an invasive intervention for a number of patients.

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Section: Discussionmentioning

confidence: 99%

The diagnostic value of a new formula combining age and prostate volume in prostate cancer

Yang¹,

Shan²,

Qiang³

et al. 2021

J. Mens. Health

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“…It is noteworthy that proteins of two different groups are often described to be present together in the cytoplasm of the same cell. For instance, platelets possess an actin filament-length regulator, gelsolin, and an actin-gelation protein, alpha-actinin (45). The cytoplasm of macrophages contains gelsolin and an actincrosslinking protein (19).…”

Section: Discussionmentioning

confidence: 99%

Secretory cell actin-binding proteins: identification of a gelsolin-like protein in chromaffin cells.

Bader¹,

Trifaró²,

Langley³

et al. 1986

The Journal of Cell Biology

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Abstract. Chr, omaffin cells, secretory cells of the adrenal medulla, have been shown to contain actin and other contractile proteins, which might be involved in the secretory process.Actin and Ca++-sensitive actin-binding proteins were purified from bovine adrenal medulla on affinity columns using DNase-I as a ligand. Buffers that contained decreasing Ca ++ concentrations were used to elute three major proteins of 93, 91, and 85 kD. The bulk of the actin was eluted with guanidine-HCl buffer plus some 93-and 91-kD proteins. These Ca ÷+-sensitive regulatory proteins were shown to inhibit the gelation of actin using the low-shear falling ball viscometer and by electron microscopy. Actin filaments were found to be shortened by fragmentation.Using antibody raised against rabbit lung macrophage gelsolin, proteolytic digestion with Staphylococcus V8 protease and two-dimensional gel electrophoresis, the 9 l-kD actin-binding protein was shown to be a gelsolin-like protein. The 93-kD actin-binding protein also showed cross-reactivity with anti-gelsolin antibody, similar peptide maps, and a basic-shift in pHi indicating that this 93-kD protein is a brevin-like protein, derived from blood present abundantly in adrenal medulla. Purification from isolated chromaffin cells demonstrated the presence of 91-and 85-kD proteins, whereas the 93-kD protein was hardly detectable.The 85-kD protein is not a breakdown product of brevin-like or gelsolin-like proteins. It did not crossreact with anti-gelsolin antibody and showed a very different peptide map after mild digestion with V8 protease. Antibodies were raised against the 93-and 91-kD actin-binding proteins and the 85-kD actinbinding protein. Antibody against the 85-kD protein did not cross-react with 93-and 9 l-kD proteins and vice versa.In vivo, the cytoskeleton organization of chromaffin secretory cells is not known, but appears to be under the control of the intracellular concentration of free calcium. The ability of calcium to activate the gelsolin-like protein, and as shown elsewhere to alter fodrin localization, provides a mechanism for gel-sol transition that might be essential for granule movement and membrane-membrane interactions involved in the secretory process.

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“…Platelet actinin was shown to be Ca ++ -sensitive [14] and had probably been assumed in most studies to be the Ca ++ -sensitive, non-muscle isoform of actinin-1. Indeed, the expression of this isoform in platelets was confirmed by cDNA sequencing [15].…”

Section: Actinin Genes and Proteinsmentioning

confidence: 99%

“…The involvement of actinin in platelet activation was apparent from early studies describing actinin as an abundant~100 kDa protein component of the platelet cytoskeleton that is involved in the bundling of actin microfilaments within pseudopods of activated platelets [20][21][22]. Platelet actinin was shown to bundle actin filaments in a Ca ++ -sensitive manner [14], though the potential presence of multiple actinin isoforms in platelets with subtle differences in their Ca ++ sensitivity was apparent [23]. In the absence of complete amino acid sequences for the various actinins, these studies employed the nomenclature a, b, c based on molecular mass [23].…”

Section: Actinin In Platelet Activation and Adhesionmentioning

confidence: 99%

The Importance of Alpha-Actinin Proteins in Platelet Formation and Function, and Their Causative Role in Congenital Macrothrombocytopenia

O’Sullivan

Cahill

Young

2021

IJMS

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The actin cytoskeleton plays a central role in platelet formation and function. Alpha-actinins (actinins) are actin filament crosslinking proteins that are prominently expressed in platelets and have been studied in relation to their role in platelet activation since the 1970s. However, within the past decade, several groups have described mutations in ACTN1/actinin-1 that cause congenital macrothrombocytopenia (CMTP)—accounting for approximately 5% of all cases of this condition. These findings are suggestive of potentially novel functions for actinins in platelet formation from megakaryocytes in the bone marrow and/or platelet maturation in circulation. Here, we review some recent insights into the well-known functions of actinins in platelet activation before considering possible roles for actinins in platelet formation that could explain their association with CMTP. We describe what is known about the consequences of CMTP-linked mutations on actinin-1 function at a molecular and cellular level and speculate how these changes might lead to the alterations in platelet count and morphology observed in CMTP patients. Finally, we outline some unanswered questions in this area and how they might be addressed in future studies.

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Isolation and characterization of a calcium-sensitive alpha-actinin-like protein from human platelet cytoskeletons.

Cited by 93 publications

References 29 publications

The diagnostic value of a new formula combining age and prostate volume in prostate cancer

The diagnostic value of a new formula combining age and prostate volume in prostate cancer

Secretory cell actin-binding proteins: identification of a gelsolin-like protein in chromaffin cells.

The Importance of Alpha-Actinin Proteins in Platelet Formation and Function, and Their Causative Role in Congenital Macrothrombocytopenia

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