Isolation and Characterization of a Bacillus velezensis D-18 Strain, as a Potential Probiotic in European Seabass Aquaculture

Monzón-Atienza, Luis; Bravo, Jimena; Torrecillas, Silvia; Montero, Daniel; Canales, Ana; Banda, Inés García de la; Galindo-Villegas, Jorge; Ramos-Vivas, José; Acosta, F.

doi:10.1007/s12602-021-09782-8

Cited by 21 publications

(9 citation statements)

References 46 publications

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“…Isolate 24 was submitted to GenBank under the gene accession number MW931747, which is closely related to B. velezensis (MZ373177) (Figure 3A). B. velezensis was first isolated from water in southern Spain as a surfactant producer 35 as well as recently from a fish farm in aquaculture used as probiotics 36 . As we acknowledge none of the scientists have investigated SA production using B. velezensis .…”

Section: Resultsmentioning

confidence: 99%

“…B. velezensis was first isolated from water in southern Spain as a surfactant producer 35 as well as recently from a fish farm in aquaculture used as probiotics. 36 As we acknowledge none of the scientists have investigated SA production using B. velezensis. This finding explores the innovation and application of nonreported microorganisms that produced a high amount of SA.…”

Section: Identification Of Selected Sa-producing Microorganismsmentioning

confidence: 99%

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Isolation and screening of microorganisms for high yield of succinic acid production

Nagime

Upaichit

Chiersilp

et al. 2022

Biotech and App Biochem

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This study involves the isolation of succinic acid (SA)‐producing microorganisms from different samples, including the rumen, sludge, soil, and wastewater. For primary screening, 29 isolates exhibited a zone of clearance around the colony, indicating acid production. For secondary screening using thin‐layer chromatography, only two isolates symbolized SA production according to their Rf values. These two isolates were further identified as Bacillus velezensis and Enterococcus gallinarum by phylogenetic analysis using the neighbor‐joining method. The high SA concentrations of 50.2 and 66.9 g/L were produced by B. velezensis and E. gallinarum with an SA yield of 0.836 and 1.12 g/g glucose, respectively. The high SA concentration from these newly isolated strains was achieved with a low formation of unwanted acids compared with those from Actinobacillus succinogenes ATCC 55618. Moreover, E. gallinarum was cultured in palm oil mill wastewater (POMW) and molasses, which were cheap substrates. The high SA production of 73.9 g/L with low other acids (the ratio of SA to total acids = 0.917) was achieved using POMW and molasses (80:20) as substrates.

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Section: Resultsmentioning

confidence: 99%

Section: Identification Of Selected Sa-producing Microorganismsmentioning

confidence: 99%

Isolation and screening of microorganisms for high yield of succinic acid production

Nagime

Upaichit

Chiersilp

et al. 2022

Biotech and App Biochem

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“…The interaction between surface proteins, produced by certain probiotic bacteria, and mucins creates specific properties that may inhibit the adhesion of pathogenic bacteria [81]. Regarding European sea bass, the adhesion of probiotics (Vagococcus fluvialis and Bacillus velezensis) in intestinal mucus showed excellent results compared to a control [36,49].…”

Section: Competitive Exclusion For Adhesion Sitesmentioning

confidence: 99%

Current Status of Probiotics in European Sea Bass Aquaculture as One Important Mediterranean and Atlantic Commercial Species: A Review

Monzón-Atienza,

Bravo,

Serradell

et al. 2023

Animals

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European sea bass production has increased in recent decades. This increase is associated with an annually rising demand for sea bass, which encourages the aquaculture industries to increase their production to meet that demand. However, this intensification has repercussions on the animals, causing stress that is usually accompanied by dysbiosis, low feed-conversion rates, and immunodepression, among other factors. Therefore, the appearance of pathogenic diseases is common in these industries after immunodepression. Seeking to enhance animal welfare, researchers have focused on alternative approaches such as probiotic application. The use of probiotics in European sea bass production is presented as an ecological, safe, and viable alternative in addition to enhancing different host parameters such as growth performance, feed utilization, immunity, disease resistance, and fish survival against different pathogens through inclusion in fish diets through vectors and/or in water columns. Accordingly, the aim of this review is to present recent research findings on the application of probiotics in European sea bass aquaculture and their effect on growth performance, microbial diversity, enzyme production, immunity, disease resistance, and survival in order to help future research.

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“…However, since antibiotics lead to bacterial resistance generation, their use is discouraged nowadays, and several countries with major fish-producing facilities have banned their inclusion in biosecurity plans. Therefore, to improve fish robustness in aquaculture settings, several strategies based on the use of alternative sources like phytogenics [ 210 ], live microbes [ 211 , 212 ], their metabolic products like short-chain fatty acids (SCFA) [ 213 ] and sphingolipids [ 214 ], or structural components like peptidoglycan [ 215 ] or B-glucan [ 216 ] have been extensively tested in the last century as possible therapeutics for fish. Nevertheless, despite the entire group being potentially effective, all the previous strategies lack any specificity against a particular fish disease.…”

Section: Future Perspectivesmentioning

confidence: 99%

Phage Therapy as a Focused Management Strategy in Aquaculture

Ramos-Vivas

Superio

Galindo-Villegas

et al. 2021

IJMS

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Therapeutic bacteriophages, commonly called as phages, are a promising potential alternative to antibiotics in the management of bacterial infections of a wide range of organisms including cultured fish. Their natural immunogenicity often induces the modulation of a variated collection of immune responses within several types of immunocytes while promoting specific mechanisms of bacterial clearance. However, to achieve standardized treatments at the practical level and avoid possible side effects in cultivated fish, several improvements in the understanding of their biology and the associated genomes are required. Interestingly, a particular feature with therapeutic potential among all phages is the production of lytic enzymes. The use of such enzymes against human and livestock pathogens has already provided in vitro and in vivo promissory results. So far, the best-understood phages utilized to fight against either Gram-negative or Gram-positive bacterial species in fish culture are mainly restricted to the Myoviridae and Podoviridae, and the Siphoviridae, respectively. However, the current functional use of phages against bacterial pathogens of cultured fish is still in its infancy. Based on the available data, in this review, we summarize the current knowledge about phage, identify gaps, and provide insights into the possible bacterial control strategies they might represent for managing aquaculture-related bacterial diseases.

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Isolation and Characterization of a Bacillus velezensis D-18 Strain, as a Potential Probiotic in European Seabass Aquaculture

Abstract: Isolation and characterization of a Bacillus velezensis D-18 strain, as a potential probiotic in European seabass aquaculture

Cited by 21 publications

References 46 publications

Isolation and screening of microorganisms for high yield of succinic acid production

Isolation and screening of microorganisms for high yield of succinic acid production

Current Status of Probiotics in European Sea Bass Aquaculture as One Important Mediterranean and Atlantic Commercial Species: A Review

Phage Therapy as a Focused Management Strategy in Aquaculture

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