Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods

Zhang, Huizhen; Shu, Mei; Yang, Wenyu; Pan, Hong; Tang, Men-Xuan; Zhao, Yuan‐Yang; Zhong, Chunjiu; Wu, Guoping

doi:10.1016/j.lwt.2022.114251

Cited by 10 publications

(1 citation statement)

References 40 publications

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“…Referring to the method by Zhang et al [20], the traditional plate separation method was used. The fish meat samples from day 15 were homogenized in sterile water and serially diluted.…”

Section: S Rdna Sequencingmentioning

confidence: 99%

Dynamic Changes in the Microbial Composition and Spoilage Characteristics of Refrigerated Large Yellow Croaker (Larimichthys crocea) during Storage

Li,

Liu,

Chen

et al. 2023

Foods

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The quality changes, dynamic changes in microbial composition, and diversity changes in large yellow croaker (Larimichthys crocea) during 4 °C refrigeration were studied using 16S rDNA high-throughput sequencing technology, and the total viable count (TVC), total volatile basic nitrogen (TVB-N), and thiobarbituric acid-reactive substances (TBARS) were determined. The results revealed a consistent increase in TVC, TVB-N, and TBARS levels over time. On the 9th day, TVC reached 7.43 lg/(CFU/g), while on the 15th day, TVB-N exceeded the upper limit for acceptable quality, reaching 42.56 mg/100 g. Based on the 16S rDNA sequencing results, we categorized the storage period into three phases: early storage (0th and 3rd days), middle storage (6th day), and late storage (9th, 12th, and 15th days). As the storage time increased, both the species richness and diversity exhibited a declining trend. The dominant genus identified among the spoilage bacteria in refrigerated large yellow croaker was Pseudomonas, accounting for a high relative abundance of 82.33%. A comparison was carried out of the spoilage-causing ability of three strains of Pseudomonas screened and isolated from the fish at the end of storage, and they were ranked as follows, from strongest to weakest: P. fluorescen, P. lundensis, and P. psychrophila. This study will provide a theoretical basis for extending the shelf life of large yellow croaker.

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“…Referring to the method by Zhang et al [20], the traditional plate separation method was used. The fish meat samples from day 15 were homogenized in sterile water and serially diluted.…”

Section: S Rdna Sequencingmentioning

confidence: 99%

Dynamic Changes in the Microbial Composition and Spoilage Characteristics of Refrigerated Large Yellow Croaker (Larimichthys crocea) during Storage

Li,

Liu,

Chen

et al. 2023

Foods

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Isolation and characterization of a novel phage P785, its combination with thermal processing for the biocontrol of Escherichia coli in milk

Yin,

Wang,

Zou

et al. 2024

Food Safety and Health

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Escherichia coli is one of the most important foodborne pathogens. In food industry, heat treatment is the most commonly used means of sterilization. Bacteriophage is a new type of antibacterial agent. The combination of heat stable bacteriophage and heat treatment technology to control E. coli pollution in food is a new antibacterial method. A bacteriophage P785 capable of cleaving E. coli was isolated in this study. P785 belongs to the myxoviridae family. It could cleave 21 strains of bacteria, with a cleavage capacity of 83 PFU/cell, and the optimal multiple of infection (MOI) was 0.01. It has good tolerance to high temperature, pH, ultraviolet radiation (UV), and four organic reagents. No genes encoding virulence were found in the whole genome. When P785 (MOI = 10,000) was added to milk combined with heat treatment (55°C, 30 min), the number of host bacteria was less than 1 lg (CFU/mL) in 0–15 days under the storage condition of 4°C, and no host bacteria survived in 30 days. The results showed that P785 had a good killing effect on E. coli, and its combination with thermal processing could reduce the thermal processing temperature and prolong the shelf life of food.

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Characterization of a broad-spectrum endolysin rLysJNwz and its utility against Salmonella in foods

Shen

Shu

Zhong

et al. 2023

Appl Microbiol Biotechnol

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Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods

Cited by 10 publications

References 40 publications

Dynamic Changes in the Microbial Composition and Spoilage Characteristics of Refrigerated Large Yellow Croaker (Larimichthys crocea) during Storage

Dynamic Changes in the Microbial Composition and Spoilage Characteristics of Refrigerated Large Yellow Croaker (Larimichthys crocea) during Storage

Isolation and characterization of a novel phage P785, its combination with thermal processing for the biocontrol of Escherichia coli in milk

Characterization of a broad-spectrum endolysin rLysJNwz and its utility against Salmonella in foods

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