Isolation and Characterization of A. hydrophila from the Al-Jadryia River in Baghdad (Iraq)

AL-Fatlawy, Hawraa Natiq Kabroot; AL-Hadrawy, Hazim.Aziz.

doi:10.12691/education-2-8-14

Cited by 11 publications

(6 citation statements)

References 25 publications

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“…The Lip gene was found in Aeromonas isolates at 17.14% [78], while it was highly detected (81.8%) in A. hydrophila strains recovered from diseased Nile tilapia [79]. Meanwhile, some previous studies reported that 100% of A. hydrophila possess hyl and lip genes [60,64,80].…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Prevalence, Virulence Genes, and Antibiogram of Motile Aeromonads Isolated from Nile Tilapia Fish Farms in Egypt and Assessment of their Water Quality

El-Gohary

Zahran

El-Gawad

et al. 2020

Animals

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The aquaculture industry is a fast-growing sector in Egypt; however, the progress of this industry is impeded by many challenges such as poor water quality and associated bacterial infections. Among others, Motile Aeromonas Septicemia (MAS), caused by aeromonads, is among the most important bacterial diseases affecting aquaculture due to its zoonotic potential. In the present work, motile aeromonads were isolated from water samples (n= 8) and Nile tilapia (n= 240) in four fish farms (farms I, II, III, and IV) in Kafr El-Sheikh province during the period March to August 2017. This step was followed by investigation of the prevalence and phenotypic, molecular, and histopathological characterization of aeromonads. In addition, antimicrobial susceptibility and virulence gene detection were analyzed. Interestingly, physicochemical water analysis revealed different ranges in relation to the fish farms and seasons. More importantly, Aeromonas isolates were phenotypically identified in 33.3% and 12.5% from fish and water samples, respectively. The highest prevalence of motile aeromonads (46.7%) was recorded from farm IV, and only 12.5% of water samples were positive for them. Out of 80 isolates, 65 (81.25%) were molecularly identified at the genus level using gyrase B (gyrB). The prevalence of the virulence genes detected in the isolated motile aeromonads was aerolysin (aer), 52.2%; elastase (ahp), 26.25%; hemolysin (hyl), 35%; and lipase (lip), 3.75%. The antibiogram profile revealed that the highest resistance of aeromonads isolates (80%) was recorded to chloramphenicol, kanamycin, and azithromycin. Meanwhile, lower resistance levels of 40%, 30%, and 20% were found for streptomycin, cefotaxime, and amoxicillin, respectively. The multiple antibiotic resistance (MAR) index values ranged between 0.27 and 0.82 of motile aeromonads isolates. Furthermore, the histopathological examinations of naturally diseased tilapia revealed widespread hepatocellular necrosis with diffuse, numerous rod-shaped bacteria in liver with melanomacrophages and lymphocytic depletion with edema and hemosiderosis in the spleen. Our findings provide an updated epidemiological baseline for future reference and highlight the likely role of the adverse impact of water quality in the outbreaks of motile aeromonads with special reference to virulence genes and antibiotic resistant traits.

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Section: Discussionmentioning

confidence: 99%

Investigation of the Prevalence, Virulence Genes, and Antibiogram of Motile Aeromonads Isolated from Nile Tilapia Fish Farms in Egypt and Assessment of their Water Quality

El-Gohary

Zahran

El-Gawad

et al. 2020

Animals

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“…Our finding is consistent with Attia et al [42], who found that a high number of A. hydrophila were positive for the aerA gene (81.8%) and Abd-El-Malek [43] who stated that the percent of lipase in Aeromonas isolates was 17.14%. Contrastingly, a previous study reported that A. hydrophila possesses a high percentage of the hylA gene (100%) [44]. Similarly, A. hydrophila was reported for the presence of hlyA genes (50%) in fish farms in East Delta As observed, the prevalence of A. hydrophila isolates was (65.63%), A. caviae (18.75%) and A. sobria (15.63%).…”

Section: Discussionmentioning

confidence: 63%

Prevalence, molecular Cاharacterization, virulotyping, and antibiotic resistance of motile aeromonads isolated from Nile tilapia farms at northern Egypt

Salem¹,

Zharan

Saad³

et al. 2020

Mansoura Veterinary Medical Journal

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This study was aimed to survey Aeromonas spp associated with cultured Nile tilapia Oreochromis niloticus (O. niloticus) showing signs of motile Aeromonas septicemia (MAS) at different fish farms; molecular characterization and identification of test isolates; and to test the isolates for their antimicrobial and virulence activities that contribute to its pathogenesis. Design: Observational study. Animals: 280 Nile tilapia. Procedures: Clinically diseased 280 Nile tilapia, were collected from different localities at Kafr El-Sheik and Dakahlia governorates. The clinical picture and gross lesions were recorded. Aeromonas spp were isolated and presumptively identified using API20E. The identification was confirmed using PCR. Hemolysin (hylA), lipase, and aerolysin (aerA) virulence genes were detected among isolates obtained from different sampling sites. Besides, antimicrobial activity was reported for the identified A. hydrophila. Results: General septicemic signs were evident on Nile tilapia including, skin hemorrhages and ulcerations, bilateral exophthalmia, congested internal organs with significant mortalities. The prevalence of bacterial infection among naturally diseased Nile tilapia was 79.17, 70, and 58.33 in Kafr El-Sheikh, El-Manzala, and Gamsa fish farms, respectively. The most prevalent bacterial isolates were aeromonads (29.84 %), of all, 65.63 A. hydrophila, 18.75 A. caviae, and 15.63 A. sobria. All isolates were positively amplified using a species-specifying primer to determine A. hydrophila. Virulence genes detection revealed that five A. hydrophila isolates (83.3 %) harbored the aerA gene. Meanwhile, hylA and lipase genes positive isolates were lower reaching 16.7 % for both genes. A. hydrophila was highly sensitive to ciprofloxacin, amikacin, trimethoprim, and chloramphenicol, and MAR index of A. hydrophila isolates was ranged from 0.16-0.42 Conclusion and clinical relevance: Our findings demonstrate that Aeromonas spp are among the bacterial pathogens implicated in summer mortalities in tilapia fish farms in Egypt. Besides, determination of the prevalence, virulence genes, and antibiotic resistance pattern associated with the disease outbreaks is critical data that warrant the development of strategies to proper monitoring and farm management practices.

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“…Smears were taken aseptically using sterile loop from kidney, liver and spleen and streaked to MacConkey agar plates. After streaking the plates were incubated at 37°C for 24 hours following the procedure described in 20 and 16 .…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

Phenotypic Characterization, Genetic Analysis and Antibiotic Sensitivity of Aeromonas Hydrophila Isolates Causing Abdominal Dropsy in Labeo Rohita Collected From Punjab, Pakistan

Sughra

Muhammad

Abbas

et al. 2021

Preprint

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Motile Aeromonas Septicaemia (MAS) is a common fresh water fish disease and major threat to the aquaculture in Pakistan. The present study was carried out on suspected cases of Rohu (Labeo rohita) to characterize and genetically analyze local strains of Aeromonas (A.) hydrophila, a key pathogen responsible for the said disease in farmed fish of Pakistan. A total of ninety suspected samples were collected from fish farms in various districts of Punjab from June 2018 to April 2019. The samples were processed and A. hydrophila strains were isolated. The primary identification of sixty seven isolates was verified by colony morphology, microscopy and phenotypic characterization with ten biochemical reactions. The A. hydrophila strains of test samples were characterized by polymerase chain reaction (PCR) using 16S rRNA at desired size of 356 bp. The PCR amplified product was subjected to DNA sequencing and phylogenetic analysis showed homology with related strains of Aeromonas spp. By antibiotic sensitivity test, the isolates were checked for nine antibiotics, in which pathogen was sensitive to four and resistant to five drugs. Results of molecular characterization confirmed strains as A. hydrophila which are useful to take preventive measures against the said disease.

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Isolation and Characterization of A. hydrophila from the Al-Jadryia River in Baghdad (Iraq)

Cited by 11 publications

References 25 publications

Investigation of the Prevalence, Virulence Genes, and Antibiogram of Motile Aeromonads Isolated from Nile Tilapia Fish Farms in Egypt and Assessment of their Water Quality

Investigation of the Prevalence, Virulence Genes, and Antibiogram of Motile Aeromonads Isolated from Nile Tilapia Fish Farms in Egypt and Assessment of their Water Quality

Prevalence, molecular Cاharacterization, virulotyping, and antibiotic resistance of motile aeromonads isolated from Nile tilapia farms at northern Egypt

Phenotypic Characterization, Genetic Analysis and Antibiotic Sensitivity of Aeromonas Hydrophila Isolates Causing Abdominal Dropsy in Labeo Rohita Collected From Punjab, Pakistan

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