Isolation and characterization of a canine rotavirus

Abstract

Canine rotavirus particles were visualized by direct electron microscopy in the feces from a clinically normal dog. The virus was subsequently propagated in cell cultures; it was characterized and compared with rotaviruses from other species. Replication of the virus in cell culture was found to be less dependent upon trypsin than that of human, bovine and porcine rotaviruses. Reproducible, sharp-edged plaques of various sizes were produced by the canine rotavirus in an established cell line of fetal rhesus monkey kidney, MA 104, under overlays of carboxymethyl cellulose or agarose. Intracytoplasmic inclusion bodies of different sizes and shapes were produced in infected MA 104 cells. By plaque reduction neutralization assay, a two-way antigenic relationship was found between the canine (CU-1) and simian (rhesus MMU 18006 and SA-11) rotaviruses. The canine rotavirus had a one-way antigenic relationship with feline (Taka), bovine (NCDV), and porcine (OSU) rotaviruses.

Isolation and characterization of rotavirus from feral pigeon in mammalian cell cultures

“…Cultures must be prepared each time from chick tissues and examined for miscellaneous adventitious viruses. To counter these disadvantages, we used MA-104 cells which have been used for the isolation and growth of many mammalian rotaviruses Fukusho, Shimizu & Ito, 1981;Hoshino et al 1982Hoshino et al , 1983. More recently, the MA-104 cell line was successfully used for the direct isolation of avian rotavirus by two groups (Theil, Reynolds & Saif, 1986;Kang, Nagaraja & Newman, 1986) Isolation of pigeon rotavirus in MA-104 cells 491 the two continuous cell lines and was not accompanied by CPE.…”

“…Rotaviruses have been isolated from a wide variety of mammalian and avian species with enteritis and diarrhoea, and also from asymptomatic animals throughout the world (McNulty, 1987;Flewett & Woode, 1978;McNulty et al 1979;Fukusho, Shimizu & Ito, 1981;Hoshino et al 1982;Hoshino et al 1983).…”

Characterization of a second bovine rotavirus serotype

“…Several serotypes have been identified among rotaviruses ti:om children (2,36), calves (5,16,27,30,33,35), foals (13), piglets (3), mice (12) and avian species (26). In calves about 90 per cent of rotavirus isolates belong to one serotype represented by bovine rotavirus (BI~V) UK (33,35).…”

Cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures

“…Similarly, McNulty et al (1980) also isolated trypsinindependent avian rotaviruses in CEL cells. Furthermore, recently, cytopathic human (Agliano et al, 1985) and canine (Hoshino et al, 1982) rotaviruses have been isolated in cell cultures without the aid of trypsin. It is unknown whether this trypsin-independent cytopathogenicity of the F-29 strain is unique to this virus strain or if this is a common characteristic of all duck rotaviruses.…”