Isolation and characterization of a multipotent clone of human embryonal carcinoma cells

Pera, Martin F.; Cooper, Stephen R.; Mills, Judith; Parrington, Jennifer M.

doi:10.1111/j.1432-0436.1989.tb00602.x

Cited by 111 publications

(75 citation statements)

References 45 publications

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“…ES cells from the two species also show different growth regulation. Whereas both mouse and hES cells require feeder cell support, leukemia inhibitory factor (LIF) cannot substitute for a feeder cell layer in maintaining hES cells Thomson et al, 1998) or embryonal carcinoma cells (Pera et al, 1989). The basis of this lack of response to LIF is unclear; some researchers have attributed it to the poor activation of the STAT3 pathway in human cells following receptor engagement (Sato et al, 2004), while others suggest that it is because of the absence, or relatively low level of expression, of components of the LIF pathway (Ginis et al, 2004;Richards et al, 2004).…”

Section: Comparing Pluripotent Mouse and Human Stem Cellsmentioning

confidence: 99%

Human embryonic stem cells: prospects for development

2004

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It is widely anticipated that human embryonic stem (ES) cells will serve as an experimental model for studying early development in our species, and,conversely, that studies of development in model systems, the mouse in particular, will inform our efforts to manipulate human stem cells in vitro. A comparison of primate and mouse ES cells suggests that a common underlying blueprint for the pluripotent state has undergone significant species-specific modification. As we discuss here, technical advances in the propagation and manipulation of human ES cells have improved our understanding of their growth and differentiation, providing the potential to investigate early human development and to develop new clinical therapies.

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Section: Comparing Pluripotent Mouse and Human Stem Cellsmentioning

confidence: 99%

Human embryonic stem cells: prospects for development

2004

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“…Many of these human lines showed little capacity for differentiation, but they provided the basis for the identi cation of a number of characteristic features of human EC cells. Eventually, several human EC cell lines capable of differentiation were obtained, including: GCT27 (Pera et al 1989;Roach et al 1993Roach et al , 1994Pera & Herszfeld 1998), NCCIT (Teshima et al 1988;Damjanov et al 1993) and NCG.R3 (Hata et al 1989;Umezawa et al 1996) and, ironically, TERA2 (Andrews et al 1984b), one of the oldest extant human teratocarcinoma cell lines. The pluripotent character of TERA2, and indeed its identity as an EC cell line, was overlooked for some time (e.g.…”

Section: Human Ec and Es Cell Linesmentioning

confidence: 99%

From teratocarcinomas to embryonic stem cells

Andrews

2002

Phil. Trans. R. Soc. Lond. B

245

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The recent derivation of human embryonic stem (ES) cell lines, together with results suggesting an unexpected degree of plasticity in later, seemingly more restricted, stem cells (so-called adult stem cells), have combined to focus attention on new opportunities for regenerative medicine, as well as for understanding basic aspects of embryonic development and diseases such as cancer. Many of the ideas that are now discussed have a long history and much has been underpinned by the earlier studies of teratocarcinomas, and their embryonal carcinoma (EC) stem cells, which present a malignant surrogate for the normal stem cells of the early embryo. Nevertheless, although the potential of EC and ES cells to differentiate into a wide range of tissues is now well attested, little is understood of the key regulatory mechanisms that control their differentiation. Apart from the intrinsic biological interest in elucidating these mechanisms, a clear understanding of the molecular process involved will be essential if the clinical potential of these cells is to be realized. The recent observations of stem-cell plasticity suggest that perhaps our current concepts about the operation of cell regulatory pathways are inadequate, and that new approaches for analysing complex regulatory networks will be essential.

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“…Several of these lines were able to differentiate extensively both in vitro and in vivo, in xenograft tumors grown in immunosuppressed mice Andrews, 1984;Pera et al, 1989;Damjanov et al, 1993). Nevertheless, human embryonic stem cells, when derived directly from human embryos, proved to have a much wider capacity to differentiate, and to be karyotypically diploid, opening up the prospects for their use in regenerative medicine (Thomson et al, 1998;Reubinoff et al, 2000).…”

Section: Pluripotent Human Stem Cells: Standing On the Shoulders Of Gmentioning

confidence: 99%