Isolation and Characterization of A Cytosolic Pyruvate Kinase cDNA From Loquat (Eriobotrya japonica Lindl.)

Qin, Qiaoping; Kaas, Quentin; Zhang, Lanlan; Xu, Kai; Li, Nanyi; Zheng, Wenjie; Lai, Qixian

doi:10.1007/s11105-012-0479-6

Cited by 18 publications

(25 citation statements)

References 42 publications

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“…Our study shows that, although in loquat both sucrose and sorbitol are important photosynthates in loquat, the sorbitol content of the fruit is high early on, but decreases significantly during development which corresponds to the period of fructose and glucose accumulation in the maturing fruit. However, fruit sucrose is only slightly decreased at maturity, which indicates that sorbitol cleavage may contribute more to the hexose pool than sucrose cleavage in the maturing loquat fruit (Qin et al 2012(Qin et al , 2013. Following long-distance transport, sucrose can be converted to fructose and glucose in the cell wall spaces by CWIN.…”

Section: Discussionmentioning

confidence: 99%

“…The sucrose, fructose and glucose in the loquat fruit and in transgenic leaves were extracted and measured by HPLC according to Qin et al (2013).…”

Section: Assay Of Enzymatic Activity and Sugar Contentmentioning

confidence: 99%

“…Trees of the Rosaceae family are unusual in that they synthesise both sorbitol and sucrose in their source leaves, and both are translocated and stored (or utilized) in their fruit. The principal sugars in loquat fruit are fructose, glucose, sucrose, and sorbitol (sugar alcohol) (Shaw and Wilson 1981;Bantog et al 1999;Hasegawa et al 2010;Qin et al 2013). Although the balance varies among cultivars, sucrose accounts for up to 35 % of the total during early fruit development, around 40 % at maturity (Bantog et al 1999;Qin et al 2012Qin et al , 2013 Hence, sucrose is of great importance not only physiologically (for fruit development) but also organoleptically (it dominates the flavour of mature loquat fruit).…”

Section: Introductionmentioning

confidence: 99%

“…The principal sugars in loquat fruit are fructose, glucose, sucrose, and sorbitol (sugar alcohol) (Shaw and Wilson 1981;Bantog et al 1999;Hasegawa et al 2010;Qin et al 2013). Although the balance varies among cultivars, sucrose accounts for up to 35 % of the total during early fruit development, around 40 % at maturity (Bantog et al 1999;Qin et al 2012Qin et al , 2013 Hence, sucrose is of great importance not only physiologically (for fruit development) but also organoleptically (it dominates the flavour of mature loquat fruit). In our previous studies, enzymes involved in loquat glycolysis have been isolated and characterised at the molecular level, including pyruvate kinase (PK) (Qin et al 2013) and hexokinase (HXK) (Qin et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Although the balance varies among cultivars, sucrose accounts for up to 35 % of the total during early fruit development, around 40 % at maturity (Bantog et al 1999;Qin et al 2012Qin et al , 2013 Hence, sucrose is of great importance not only physiologically (for fruit development) but also organoleptically (it dominates the flavour of mature loquat fruit). In our previous studies, enzymes involved in loquat glycolysis have been isolated and characterised at the molecular level, including pyruvate kinase (PK) (Qin et al 2013) and hexokinase (HXK) (Qin et al 2012). Given the important roles of VIN in addition to enzymes involved in glycolysis, this study isolated and characterised a fulllength cDNA encoding putative VIN from loquat.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of a loquat (Eriobotrya japonica Lindl.) vacuolar invertase affects sucrose levels and growth

Wang

Feng²,

Qin

et al. 2015

Plant Cell Tiss Organ Cult

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Sucrose and its cleavage products are essential molecules in higher plants. Vacuolar invertase (VIN) is a type of invertase that is specifically localised in the vacuole. It irreversibly cleaves sucrose into glucose and fructose, and plays important roles in sugar accumulation or cell expansion. In this study, a full-length cDNA of the VIN gene, EjVIN, was isolated from loquat (Eriobotrya japonica Lindl.). The EjVIN encoding protein possesses typical and highly conserved motifs among plant VINs, including the b-fructosidase motif (NDPNG), catalytic domain (WECVD) and a possible vacuolar targeting motif (ILPN). Phylogenic analyses showed that EjVIN is close to apple and pear VIN, falling within the VIN subgroup. EjVIN transcript and acid invertase activity were highest in the early stages of fruit development and in mature fruit.The transcript of EjVIN was significantly enhanced in vivo after 3 h of treatment with fructose or glucose. The transgenic tobacco plants overexpressed EjVIN grew faster with decreased size. Further analyses indicate that the sucrose levels were decreased, but fructose levels were slightly increased in immature transgenic plants compared with wild type plants. The results indicate that EjVIN play more important role during early loquat fruit development stage. Overexpression of loquat EjVIN accelerated sucrose hydrolysis, affected plant growth, which possibly due to a modification of the metabolic fluxes in the transgenic plants at early development stage.

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Section: Discussionmentioning

confidence: 99%

“…The sucrose, fructose and glucose in the loquat fruit and in transgenic leaves were extracted and measured by HPLC according to Qin et al (2013).…”

Section: Assay Of Enzymatic Activity and Sugar Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Overexpression of a loquat (Eriobotrya japonica Lindl.) vacuolar invertase affects sucrose levels and growth

Wang

Feng²,

Qin

et al. 2015

Plant Cell Tiss Organ Cult

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Isolation and Characterization of a Lipid Transfer Protein Gene (BplLTP1) from Betula platyphylla

et al. 2013

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Characterisation of the subunit genes of pyrophosphate-dependent phosphofructokinase from loquat (Eriobotrya japonica Lindl.)

Qin

Kaas

Wu³

et al. 2014

Tree Genetics & Genomes

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Pyrophosphate-dependent phosphofructokinase (PFP) catalyses the reversible phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate in the glycolysis pathway. Two full-length complementary DNAs encoding putative PFP α-and β-subunits, named EjPFPa and EjPFPb1, were isolated by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE) from Eriobotrya japonica Lindl. (loquat). The other β-subunit gene was identified from transcriptome data obtained by Illumina sequencing, designated as EjPFPb2. They share up to 88 % identity with other plant PFP α/β-subunits. EjPFPa and EjPFPb clustered separately in clades of plant PFP α-or β-subunits, respectively. EjPFPa and EjPFPb were both shown to be mainly localised in the cell membrane using confocal microscopy of GFP fusion proteins 35S:EjPFPa/b-GFP. Monitoring the dynamic changes of transcripts and proteins demonstrated that EjPFPa and EjPFPb did not show coordinated expression during fruit development in loquat. The transcript levels of EjPFPa and EjPFPb in the leaves of loquat seedlings were significantly enhanced after 3 h of treatment with 0.5, 1.0 and 1.5 M fructose or glucose, which indicates that EjPFPa and EjPFPb are modulated by fructose and glucose in vivo. Transgenic tobacco plants overexpressed EjPFPa did not show visible phenotype changes, while plants overexpressing EjPFPb1 grew faster at the cost of reduced leaf size and sucrose content. Meanwhile, fructose content increased in growing plants compared with wild-type plants.The results confirm that genes of the two subunits from loquat PFP have different transcriptional regulation systems and play different roles in carbohydrate metabolism. Elevation of the PFP β-subunit gene expression can partly impact the glycolytic carbon allocation in growing plants.

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Isolation and Characterization of A Cytosolic Pyruvate Kinase cDNA From Loquat (Eriobotrya japonica Lindl.)

Cited by 18 publications

References 42 publications

Overexpression of a loquat (Eriobotrya japonica Lindl.) vacuolar invertase affects sucrose levels and growth

Overexpression of a loquat (Eriobotrya japonica Lindl.) vacuolar invertase affects sucrose levels and growth

Isolation and Characterization of a Lipid Transfer Protein Gene (BplLTP1) from Betula platyphylla

Characterisation of the subunit genes of pyrophosphate-dependent phosphofructokinase from loquat (Eriobotrya japonica Lindl.)

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