Isolation and characterization of acetic acid-tolerant galactose-fermenting strains of Saccharomyces cerevisiae from a spent sulfite liquor fermentation plant

Lindén, Torbjörn; Peetre, Jaak; Hahn‐Hägerdal, Bärbel

doi:10.1128/aem.58.5.1661-1669.1992

Cited by 90 publications

(30 citation statements)

References 34 publications

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“…In addition to CEN.PK, we selected strains ATCC 96581, MUCL 11987, and Ethanol Red. ATCC 96581 has been the subject of several studies determining the performance of S. cerevisiae in lignocellulosic hydrolysates (Linden et al, 1992;Palmqvist et al, 1999;Brandberg et al, 2004Brandberg et al, , 2007Hou & Yao, 2012) and showed the shortest latency phase of all strains included in our screening. Ethanol Red is a widely used strain in bioethanol production and is therefore of industrial relevance.…”

Section: Saccharomyces Cerevisiae Intraspecies Diversity With Regard mentioning

confidence: 99%

The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance inSaccharomyces cerevisiae

et al. 2014

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High acetic acid tolerance of Saccharomyces cerevisiae is a relevant phenotype in industrial biotechnology when using lignocellulosic hydrolysates as feedstock. A screening of 38 S. cerevisiae strains for tolerance to acetic acid revealed considerable differences, particularly with regard to the duration of the latency phase. To understand how this phenotype is quantitatively manifested, four strains exhibiting significant differences were studied in more detail. Our data show that the duration of the latency phase is primarily determined by the fraction of cells within the population that resume growth. Only this fraction contributed to the exponential growth observed after the latency phase, while all other cells persisted in a viable but non-proliferating state. A remarkable variation in the size of the fraction was observed among the tested strains differing by several orders of magnitude. In fact, only 11 out of 10(7) cells of the industrial bioethanol production strain Ethanol Red resumed growth after exposure to 157 mM acetic acid at pH 4.5, while this fraction was 3.6 × 10(6) (out of 10(7) cells) in the highly acetic acid tolerant isolate ATCC 96581. These strain-specific differences are genetically determined and represent a valuable starting point to identify genetic targets for future strain improvement.

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Section: Saccharomyces Cerevisiae Intraspecies Diversity With Regard mentioning

confidence: 99%

The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance inSaccharomyces cerevisiae

et al. 2014

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“…The six S. cerevisiae strains used in the study were Baker's yeast (Jästbolaget AB, Rotebro, Sweden), CBS 8066 (Verduyn et al, 1990), CEN.PK 113-7D (SRD, Oberursel, Germany), TMB3000 (Linden et al, 1992), TMB3500 (J.R.M. Almeida, unpublished works) and USM21 (Vanderwesthuizen and Pretorius, 1992).…”

Section: Yeast Strains Medium and Hydrolysatementioning

confidence: 99%

Variability of the response of Saccharomyces cerevisiae strains to lignocellulose hydrolysate

Modig

Almeida

Gorwa-Grauslund

et al. 2008

Biotech & Bioengineering

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The development of tolerant microorganisms is needed for the efficient fermentation of inhibitory lignocellulose hydrolysates. In the current work, the fermentation performance of six selected strains of Saccharomyces cerevisiae in dilute-acid spruce hydrolysate was compared using two different modes of fermentation; either single pulse addition of hydrolysate to exponentially growing cells or continuous feeding of the same amount of hydrolysate in a controlled fed-batch fermentation was made. All strains performed better in fed-batch mode than when all hydrolysate was added at once. However, the difference between strain performances varied significantly in the two fermentation modes. Large differences were observed between strains during the fed-batch experiments in the in vitro ability to reduce the furan compounds furfural and 5-hydroxymethyl furfural (HMF). A common feature among the strains was the induction of NADPH-coupled reduction of furfural and HMF, with the exception of strain CBS 8066. This strain also performed relatively poorly in both batch and fed-batch fermentations. Strain TMB3000--previously isolated from spent sulphite liquor fermentation--was by far the most efficient strain with respect to specific fermentation rate in both pulse addition and fed-batch mode. This strain was the only strain showing a significant constitutive NADH-coupled in vitro reduction of HMF. The ability to induce NADPH-coupled reduction together with the level of the apparently constitutive NADH-coupled reduction appeared to be key factors for selecting a suitable strain for fed-batch conversion of lignocellulose hydrolysate.

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“…In the present investigation we compared two strains of S. cerevisiae ; TMB3000, known to be inhibitor‐tolerant (Lindén et al , 1992; Martín and Jönsson, 2003; Brandberg et al , 2004), and a laboratory strain, CBS8066, using genome‐wide transcription analysis of known yeast reductase and dehydrogenase genes (Stewart et al , 2001; Katz et al , 2003). Selected genes from the transcription analysis were evaluated using the Ex‐Clone overexpression collection.…”

Section: Introductionmentioning

confidence: 99%

A 5‐hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance

Petersson

Almeida

Modig

et al. 2006

Yeast

253

181

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The fermentation of lignocellulose hydrolysates by Saccharomyces cerevisiae for fuel ethanol production is inhibited by 5-hydroxymethyl furfural (HMF), a furan derivative which is formed during the hydrolysis of lignocellulosic materials. The inhibition can be avoided if the yeast strain used in the fermentation has the ability to reduce HMF to 5-hydroxymethylfurfuryl alcohol. To enable the identification of enzyme(s) responsible for HMF conversion in S. cerevisiae, microarray analyses of two strains with different abilities to convert HMF were performed. Based on the expression data, a subset of 15 reductase genes was chosen to be further examined using an overexpression strain collection. Three candidate genes were cloned from two different strains, TMB3000 and the laboratory strain CEN.PK 113-5D, and overexpressed using a strong promoter in the strain CEN.PK 113-5D. Strains overexpressing ADH6 had increased HMF conversion activity in cellfree crude extracts with both NADPH and NADH as co-factors. In vitro activities were recorded of 8 mU/mg with NADH as co-factor and as high as 1200 mU/mg for the NADPH-coupled reduction. Yeast strains overexpressing ADH6 also had a substantially higher in vivo conversion rate of HMF in both aerobic and anaerobic cultures, showing that the overexpression indeed conveyed the desired increased reduction capacity.

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Isolation and characterization of acetic acid-tolerant galactose-fermenting strains of Saccharomyces cerevisiae from a spent sulfite liquor fermentation plant

Cited by 90 publications

References 34 publications

The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance inSaccharomyces cerevisiae

The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance inSaccharomyces cerevisiae

Variability of the response of Saccharomyces cerevisiae strains to lignocellulose hydrolysate

A 5‐hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance

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