Isolation and characterization of alborixin from Streptomyces scabrisporus: A potent cytotoxic agent against human colon (HCT-116) cancer cells

Shah, Aabid Manzoor; Wani, Abubakar; Qazi, Parvaiz Hassan; Rehman, Shakeel-u; Mushtaq, Saleem; Ali, Shiekh Abid; Hussain, Aashiq; Shah, Aabid Manzoor; Qazi, Asif Khurshid; Makhdoomi, Ubaid; Hamid, Abid; Kumar, Ajay

doi:10.1016/j.cbi.2016.06.032

Cited by 26 publications

(17 citation statements)

References 29 publications

(26 reference statements)

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“…Alborixin is reported to have strong antimicrobial activity against grampositive bacteria [22]. It has also been shown to possess anticancer activity against human colon cancer cells [23]. Literature survey revealed that some of the ionophore antibiotics have already been known to induce autophagy [24].…”

Section: Introductionmentioning

confidence: 99%

Alborixin clears amyloid-β by inducing autophagy through PTEN-mediated inhibition of the AKT pathway

et al. 2019

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Imbalance in production and clearance of amyloid beta (Aβ) is the primary reason for its deposition in Alzheimer disease. Macroautophagy/autophagy is one of the important mechanisms for clearance of both intracellular and extracellular Aβ. Here, through screening, we identified alborixin, an ionophore, as a potent inducer of autophagy. We found that autophagy induced by alborixin substantially cleared Aβ in microglia and primary neuronal cells. Induction of autophagy was accompanied by up regulation of autophagy proteins BECN1/Beclin 1, ATG5, ATG7 and increased lysosomal activities. Autophagy induced by alborixin was associated with inhibition of the phosphoinositide 3-kinase (PI3K)-AKT pathway. A knock down of PTEN and consistent, constitutive activation of AKT inhibited alborixin-induced autophagy and consequent clearance of Aβ. Furthermore, clearance of Aβ by alborixin led to significant reduction of Aβ-mediated cytotoxicity in primary neurons and differentiated N2a cells. Thus, our findings put forward alborixin as a potential anti-Alzheimer therapeutic lead.

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Section: Introductionmentioning

confidence: 99%

Alborixin clears amyloid-β by inducing autophagy through PTEN-mediated inhibition of the AKT pathway

et al. 2019

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“…Streptomyces scabrisporus NF3, an endophytic actinomycete, isolated from the tree Amphipterygium adstringens , in Mexico (1), exhibits the potential to produce diverse bioactive compounds, for instance, the antibacterial hitachimycin (2) and the antitumoral alborixin (3). This species encompasses aerobic sporulating Gram-positive bacteria with a high G+C content (69 to 73%) (4).…”

Section: Genome Announcementmentioning

confidence: 99%

“…This species encompasses aerobic sporulating Gram-positive bacteria with a high G+C content (69 to 73%) (4). While other strains of the species have been isolated and characterized previously (3–6), only strain S. scabrisporus DSM 41855 was sequenced so far. The genome of this strain is composed of 199 contigs, making comparative analyses problematic.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Streptomyces scabrisporus NF3, an Endophyte Isolated from Amphipterygium adstringens

et al. 2017

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“…UPMRS4 which had antimicrobial activities . Likewise, actinomycin C complex extracted from S. pratensis effectively controlled S. aureus . Actinomycin C2 and actinomycin C3 isolated from red soil Actinomyces effectively inhibit the growth of Micrococcus luteus and S. aureus .…”

Section: Discussionmentioning

confidence: 95%

Antimicrobial potential of haloalkaliphilic Nocardiopsis sp. AJ1 isolated from solar salterns in India

et al. 2019

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Antagonistic haloalkaliphilic Nocardiopsis sp. AJ1 (GenBank JX575136.1), isolated and identified from the saline soil of Kovalam solar salterns was able to produce antimicrobial secondary metabolites and effectively suppressed several bacterial and fungal pathogens. The metabolite extracted from ethyl acetate precipitation suppressed the bacterial and fungal pathogens to the range between 2.14 and 20.14 mm and also controlled the shrimp killer virus WSSV by 83% than the control and significantly (p < 0.05) differed. GC-MS analysis revealed that, the ethyl acetate precipitation contains pyrrolo (1,2-A(pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-) and actinomycin C2. Non ribosomal peptide synthetase (NRPS) was amplified by PCR with the amplicon size of 750-800 bp length and further predicted the secondary structure by Iterative Threading Assembly Refinement (I-TASSER) bioinformatics approach. I-TASSER prediction helped to find out the secondary, 3-D structure, and ligand binding sites. The top ten modelling concluded that, the NRPS gene is closely similar to surfactin synthesizing gene, surfactin A synthetase C (SRFA-C). The findings revealed that, the active compounds from the secondary metabolites effectively suppressed the pathogenic bacteria, fungi, and virus and useful to develop antimicrobials. K E Y W O R D S actinobacteria, antimicrobials, haloalkaliphils, Nocardiopsis sp. AJ1, non ribosomal peptide synthetase (NRPS)

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Isolation and characterization of alborixin from Streptomyces scabrisporus: A potent cytotoxic agent against human colon (HCT-116) cancer cells

Cited by 26 publications

References 29 publications

Alborixin clears amyloid-β by inducing autophagy through PTEN-mediated inhibition of the AKT pathway

Alborixin clears amyloid-β by inducing autophagy through PTEN-mediated inhibition of the AKT pathway

Draft Genome Sequence of Streptomyces scabrisporus NF3, an Endophyte Isolated from Amphipterygium adstringens

Antimicrobial potential of haloalkaliphilic Nocardiopsis sp. AJ1 isolated from solar salterns in India

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