Isolation and characterization of an algicidal bacterium against the bloom-forming algae raphidophyte <i>Heterosigma akashiwo</i>

Zhang, Zhenzhen; Hu, Guangwei; Huang, Jinwang; Chen, Lei; Yin, Yue; Cai, Yuefeng; Shen, Xin; Ji, Nanjing

doi:10.1080/09593330.2022.2036250

Cited by 11 publications

(4 citation statements)

References 44 publications

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“…Additionally, it should be noted that this research would have been more complete if it had been supplemented with data on the effects of Ba3 broth on algal cultures [16]. The purification and identification of the algicidal compounds, as well as the safety evaluation in marine environment and its impact on cultured organisms which requires further investigation [13,38].…”

Section: Discussionmentioning

confidence: 99%

Algicidal Properties of Microbial Fermentation Products on Inhibiting the Growth of Harmful Dinoflagellate Species

Balaji-Prasath

Wang

et al. 2022

Fermentation

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The fermentation processes of algicidal bacteria offer an eco-friendly and promising approach for controlling harmful algae blooms (HABs). The strain Ba3, previously isolated and identified as Bacillus sp., displays robust algicidal activity against HABs dinoflagellate in particular. Microbial fermentation products have also been found to provide metabolites with multiple bioactivities, which has been shown to reduce harmful algae species’ vegetative cells and thus reduce red tide outbreaks. In this study, the microbial fermentation of algicidal bacterium Ba3 was analyzed for its potential ability of algicidal compounds. A treatment time increased the algicidal efficiency of the fermentation products against Prorocentrum donghaiense (91%) and Alexandrium tamarense (82%). Among the treatment groups, the changing trend for the 2% treatment group was faster than that for the other treatments, showing that the inhibition rate could reach 99.1% in two days. Active components were separated by organic solvent extraction and macroporous resin, and the molecular weight of the active components was analyzed by LC-MS. The result shows that the microbial fermentation products offer a potential, not practical use for controlling the outbreaks of dinoflagellate blooms. As a result of its potential application for inhibiting HABs, these findings provide an encouraging basis for promoting large-scale fermentation production and the controlling the outbreaks of red tide.

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Section: Discussionmentioning

confidence: 99%

Algicidal Properties of Microbial Fermentation Products on Inhibiting the Growth of Harmful Dinoflagellate Species

Balaji-Prasath

Wang

et al. 2022

Fermentation

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“…is one of the most frequently reported and abundant algicidal bacterial species in the ocean. It has been documented that bacteria of this genus show algicidal effects against phytoplankton, including Dinophyceae, Raphidophyceae, and Bacillariophyceae ( Lovejoy et al, 1998 ; Lee et al, 2000 ; Zhang et al, 2022 ). Therefore, this isolate may be involved in the regulation of algal blooms.…”

Section: Discussionmentioning

confidence: 99%

“…previously reported ( Wang et al, 2020 ), which lyse target algae by releasing extracellular substances. The characteristics of algicidal compounds are influenced by various factors such as temperature and pH ( Fu et al, 2011 ; Zhang et al, 2022 ). Bai et al (2011) reported that the compounds of Brevibacterium sp.…”

Section: Discussionmentioning

confidence: 99%

“…We collected surface seawater from a phytoplankton bloom in the coastal waters of Lianyungang, China (34 • 46'57"N, 119 • 27'8"E) (Zhang et al, 2022). The water samples were serially 10.3389/fmicb.2022.1091561 diluted 10-fold with sterilized seawater, and 0.2 mL of each 10fold serially diluted dilution was spread onto 2216E (10 g L −1 of tryptone, 2 g L −1 of yeast extract, 0.2 g L −1 of ferrous sulfate, and 1.0 L seawater) agar plates and incubated at 28 • C for 48 h. The single colonies with significant differences in morphology were selected to obtain pure strains.…”

Section: Isolation Screening and Identification Of Algicidal Bacteriamentioning

confidence: 99%

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Isolation and characterization of a high-efficiency algicidal bacterium Pseudoalteromonas sp. LD-B6 against the harmful dinoflagellate Noctiluca scintillans

Yin¹,

Xu²,

Chen³

et al. 2022

Front. Microbiol.

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The dinoflagellate Noctiluca scintillans is a harmful algal species that is globally distributed and poses a certain threat to marine ecosystems. Recent research has shown that the application of algicidal bacteria is a promising method to prevent and control such harmful algal blooms (HABs), given its advantages of safety and efficiency. In this study, a strain of algicidal bacterium LD-B6 with high efficiency against N. scintillans was isolated from the coastal waters of Lianyungang, China. 16S rDNA sequence analysis showed that the strain LD-B6 belongs to the genus Pseudoalteromonas. Furthermore, the algicidal effect of LD-B6 on N. scintillans was investigated. The results showed that strain LD-B6 exerted strong algicidal activity against N. scintillans. After 12 h of bacterial culture addition to algal cultures at a 2% final volume rate, the algicidal activity reached 90.5%, and the algicidal activity of LD-B6 was influenced by the density of N. scintillans. In addition, the algicidal bacterium LD-B6 was found to indirectly lyse algal cells by secreting extracellular compounds. These algicidal compounds were stable, indicating that they are not proteins. Importantly, strain LD-B6 was broadly general, showing varying degrees of lysing effects against five of the six algal species tested. On the basis of the described studies above, the algicidal powder was also initially developed. In summary, the isolated bacterial strain LD-B6 shows the potent algicidal capability to serve as a candidate algicidal bacterium against N. scintillans blooms.

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Ecotoxicological Consequences of Non-chemical Toxic Agents in Aquatic Ecosystems: Effects of Biological Stressors

Kilinc,

dos Santos,

Hazeem

et al. 2024

The Handbook of Environmental Chemistry

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Isolation and characterization of an algicidal bacterium against the bloom-forming algae raphidophyte Heterosigma akashiwo

Cited by 11 publications

References 44 publications

Algicidal Properties of Microbial Fermentation Products on Inhibiting the Growth of Harmful Dinoflagellate Species

Algicidal Properties of Microbial Fermentation Products on Inhibiting the Growth of Harmful Dinoflagellate Species

Isolation and characterization of a high-efficiency algicidal bacterium Pseudoalteromonas sp. LD-B6 against the harmful dinoflagellate Noctiluca scintillans

Ecotoxicological Consequences of Non-chemical Toxic Agents in Aquatic Ecosystems: Effects of Biological Stressors

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