Isolation and characterization of an interleukin-8-like peptide in the bovine species

Hassfurther, R.; Canning, Peter C.; Geib, R

doi:10.1016/0165-2427(94)90001-9

Cited by 15 publications

(9 citation statements)

References 18 publications

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“…This is the first study to provide direct evidence that IL-8 and IL-1β are secreted by bovine PMN. Purified bovine peripheral blood mononuclear cells were shown to produce an IL-8 like peptide, that possessed chemotactic activity for bovine PMN [12]. Culture supernantants from purified peripheral blood PMN were previously shown to produce IL-1 like cytokines that exhibited IL-1 activity [3].…”

Section: Discussionmentioning

confidence: 99%

Shedding of sCD14 by bovine neutrophils following activation with bacterial lipopolysaccharide results in down-regulation of IL-8

et al. 2007

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-CD14, the leukocyte co-receptor for lipopolysaccharide (LPS), is important in the response of bovine polymorphonuclear neutrophil leukocytes (PMN) to Gram-negative bacteria. In other species, the expression of CD14 on the surface of PMN was shown to increase after exposure to inflammatory stimuli. These newly expressed molecules may originate from either an intracellular pool or through new gene expression. We sought to characterize bovine PMN cell surface expression and shedding of CD14 molecules, and CD14's effect on secretion of the chemoattractants IL-8 and IL-1β by PMN. Bovine PMN were incubated in RPMI for 20 h at 37 • C with LPS (1, 10, 100 µg/mL). IL-8 release increased with treatment of 1 µg/mL LPS, but decreased 41.5 and 95% at the 10 and 100 µg/mL concentrations of LPS, respectively. In contrast, shedding of CD14 from the surface of PMN only increased at the highest concentration of LPS (100 µg/mL). Secretion of IL-1β was similar regardless of the LPS concentration used to stimulate PMN. The effect of PMN concentration (1 × 10 7 , 2.5 × 10 7 , 5 × 10 7 , and 10 × 10 7 /mL) on CD14 cell surface expression and shedding of IL-8 and IL-1β were also determined. Shedding of CD14 by PMN increased with increasing concentration of PMN after exposure to 0.1 and 10 µg/mL of LPS, while secretion of IL-8 decreased. IL-1β increased at the highest concentration of PMN. The use of real time polymerase chain reaction showed that CD14 mRNA expression was not different between control and LPS-stimulated cells, indicating that the sCD14 came from either membrane bound CD14 or a preformed pool. Our results demonstrate that release of CD14 from PMN suppresses secretion of IL-8, and may be an important regulatory mechanism for controlling excessive migration of PMN into the bovine mammary gland.CD14 / IL-8 / LPS / neutrophil / bovine

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Section: Discussionmentioning

confidence: 99%

Shedding of sCD14 by bovine neutrophils following activation with bacterial lipopolysaccharide results in down-regulation of IL-8

et al. 2007

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“…Chemotactic activity of molecular mass fractions of mastitic mammary secretions. Bovine IL-8 has previously been shown to have a molecular mass of 7.8 kDa (11). We therefore fractionated mastitic samples to determine if the chemotactic activity falls within the anticipated fraction.…”

Section: Effects Of Anti-il-8 Antibodies On Neutrophil Chemotactic Acmentioning

confidence: 99%

Chemotactic Activities in Nonmastitic and Mastitic Mammary Secretions: Presence of Interleukin-8 in Mastitic but Not Nonmastitic Secretions

Barber

Yang

1998

Clin Diagn Lab Immunol

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Due to its association with low-quality milk and a decrease in milk production in bovines, mastitis is a major cause of economic loss. Additionally, mastitis can be harmful to suckling newborns and can cause damage to the mammary gland. In mastitic mammary secretions there is a substantial increase in somatic cells, specifically neutrophils. In this study we examined the ability of mastitic and nonmastitic mammary secretions to cause in vitro neutrophil chemotaxis using a microchemotaxis assay. Also, the role of the inflammatory chemokine interleukin-8 (IL-8) in neutrophil recruitment during mastitis was addressed in these in vitro experiments. We found that both nonmastitic and mastitic mammary secretions were chemotactic, not chemokinetic, for neutrophils. The neutrophil chemotactic activity in mastitic, but not nonmastitic, mammary secretions was blocked by anti-IL-8 antibodies. Molecular mass separation of the active components showed that the chemotactic activity of the mastitic secretions was present in the 10-kDa-or-less fraction and was blocked by anti-IL-8 antibodies. These results indicate that IL-8 plays a major role in neutrophil recruitment during mastitis. An understanding of its role will be of help in designing strategies for immunomodulatory therapies for mastitis.

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“…As an additional control group to exclude the effects of heat‐stable contaminants such as endotoxin, six rats were injected i.c.v. with heat‐inactivated IL‐8 (100 ng) (60 °C for 30 min) (Hassfurther et al. 1994).…”

Section: Experiments 2: Effects Of Icv Administration Of Il‐8 At Damentioning

confidence: 99%

Interleukin‐8 promotes non‐rapid eye movement sleep in rabbits and rats

García-García

Yoshida

Krueger

2004

Journal of Sleep Research

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SUMMAR Y Interleukin-8 (IL-8) is a cytokine found in the brain. In this study, the ability of IL-8 to induce sleep in rabbits and rats was investigated. Twenty-seven Sprague-Dawley rats and 16 male New Zealand White rabbits were provided electroencephalographic (EEG) electrodes, a brain thermistor, and a lateral intracerebroventricular cannula. The animals were injected intracerebroventricularly (i.c.v.) with pyrogen-free saline and, one of the following doses of IL-8 on a separate day: 1.25 or 12.5 ng in rabbits and 10, 50, or 100 ng in rats. EEG, brain temperature, and motor activity were recorded for 23 h after the i.c.v. injections. IL-8 increased time spent in non-rapid eye movement sleep (NREMS) without affecting rapid eye movement sleep (REMS). In rabbits, both doses of IL-8 promoted NREMS. In rats, the 10 and 50 ng doses of IL-8 failed to affect sleep, but the 100 ng dose of IL-8 enhanced NREMS. EEG slow-wave activity during NREMS was increased after the high dose of IL-8 in rabbits. IL-8 also induced fever in rabbits but not rats. Heat inactivated IL-8 did not alter any of the parameters measured. Current results support the notion that the brain cytokine network plays a role in sleep regulation.k e y w o r d s cytokine, fever, non-rapid eye movement sleep, slow-wave activity

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Isolation and characterization of an interleukin-8-like peptide in the bovine species

Cited by 15 publications

References 18 publications

Shedding of sCD14 by bovine neutrophils following activation with bacterial lipopolysaccharide results in down-regulation of IL-8

Shedding of sCD14 by bovine neutrophils following activation with bacterial lipopolysaccharide results in down-regulation of IL-8

Chemotactic Activities in Nonmastitic and Mastitic Mammary Secretions: Presence of Interleukin-8 in Mastitic but Not Nonmastitic Secretions

Interleukin‐8 promotes non‐rapid eye movement sleep in rabbits and rats

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