Isolation and characterization of antibiotic X-14547A, a novel monocarboxylic acid ionophore produced by Streptomyces antibioticus NRRL 8167.

Abstract

A novel carboxylic acid ionophore, antibiotic X-14547A, closely related to the polyether antibiotics has been isolated along with four other metabolites from fermented cultures of a new strain of Streptomyces antibiotrcus. The structure, determined by X-ray analysis of the R(+ )-l-amino-l-(4-bromophenyl)-ethane salt contained pyrrole carbonyl and trans-butadienyl chremophores in addition to the unusual tetrahydroindane bicyclic ring system. A second novel metabolite was identified as 3-ethyl-l,3-dihydro-3-methoxy-2H-indol-2-one.

Purification of Secondary Metabolites From Soil Actinomycetes

“…The ethyl acetate phase contains antibiotic substances separated from aqueous phase. It was evaporated to dryness in water bath and the residue obtained was used to determine antimicrobial activity [16].…”

Étude comparée de trois antibiotiques ionophores: X.537 A (lasalocide), A.23187 (calcimycine) et X.14547 A. Complexation des cations IA et IIA, transport de Ca++

“…A.23187 (ou calcimycine) est isole a partir d'une culture en fermenteur de la souche de Srreptomyces chartreusis NRRL 3882, selon un procede qui a kt6 dCcrit (12 (21).…”

“…11 s'agit ici de la premiere Ctude de complexes des alcalins avec X.14547 A. Le type de complexes obtenus est different des deux ionophores prCcCdents; il implique la participation d'une molecule d'acide et de son anion dans une association 2:l avec le cation, telle qu'on l'a deja observee dans la structure cristalline avec l'ion R(+) bromo-4 phenyl-1 ethyl ammonium (21). Cette difference de comportement est tres vraisemblablement liee a la structure particuliere du compose.…”

“…Cette difference de comportement est tres vraisemblablement liee a la structure particuliere du compose. L'examen des modeles molCculaires montre qu'une conformation repliee de la chaine ne laisse pas apparaitre une cavite complexante permettant l'accueil d'un cation, ce qui est dii en particulier a la geometrie de la molecule, imposCe par le groupement trans-butadienyle et la conformation bloquee du titrahydroindane; seule des associations du type 2:l semblent possibles a realiser si l'on se refere a l'etude cristallographique (21).…”

Antibacterial activity of actinomycetes against bacterial pathogens of diabetic foot ulcers

“…After incubation, the content of the flasks were filtered through Whatman No.1 filter paper. An antimicrobial compounds were extracted from the culture filtrate by solvent extraction method of Westly et al (1986). Ethyl acetate was added to the filtrate in the ratio of 1:1 (v/v) and shaken vigorously for 1 hour for complete extraction.…”