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Sclerotium rolfsii is a ubiquitous soil-borne fungal pathogen known to cause disease on worldwide range of agricultural and horticultural crops. In spite of economic loss caused by this pathogen, very few reports were available on this aspect, biochemical characterization of the S. rolfsii; hence the present study was under taken to study the biochemical characterization of S. rolfsii. Qualitative and quantitative analysis of free and protein-bound amino acids was carried out in mycelial mats and culture filtrate of S. rolfsii. Five amino acids were identified and were common both in free and proteinbound amino acids. Methionine was the most abundantly occurring amino acid in the protein-bound amino acids, whereas asparagine in case of free amino acids. Eleven amino acids were detected in the culture filtrate, but methionine, phenylalanine and leucine/isoleucine were more abundant. Phenolic acids both in culture filtrate and mycelia mat were analyzed by 2-dimensional paper chromatography; seven were detected both in culture filtrate and mycelial mat. Gallic, ferulic, chlorogenic and cinnamic acids were common both in mycelium and culture filtrate of the S. rolfsii. The other biochemical constituents like carbohydrate fractions, phenolic compounds and nitrogen fractions present in both mycelia mat and culture filtrate were also studied.

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Diabetes mellitus is a serious public health problem worldwide. Diabetic foot ulcers (DFU), a major complication in Type 2 diabetes are one of the major causes of morbidity and mortality around the world. To screen various bacterial pathogens present in diabetic foot ulcers and to determine their antibiotic sensitivity to actinomycetes isolated from various fields of Chandragiri, Tirupati, twenty four actinomycetes isolates were isolated and screened by primary and secondary screening methods to determine potent antibiotic producers by using test organisms. Among 24 isolates, 4 were more potent and they showed varied range of antibacterial activity of pathogens, isolated form diabetic foot ulcers. Four isolates were compared with lenezoid antibiotic. Enterococcus was resistant to lenezoid antibiotic but four actinomycetes inhibited the growth of Enterococi.