Isolation and characterization of arsenic-resistant bacteria and possible application in bioremediation

Dey, Uttiya; Chatterjee, Soumendranath; Mondal, Naba Kumar

doi:10.1016/j.btre.2016.02.002

Cited by 201 publications

(81 citation statements)

References 33 publications

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“…This molecular technique has been extensively used for bacterial phylogeny, thereby leading to the establishment of large public-domain databases and its vast exploitation in the identification of bacteria (Khusro and Aarti 2015). The identification of bacterium using 16S rRNA sequencing in the present investigation is consistent with other related current reports (Dash et al 2015;Dey et al 2016). …”

Section: Discussionsupporting

confidence: 89%

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

Aarti¹,

Khusro²,

Agastian³

2017

Bioresour. Bioprocess.

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Background:In modern times, bacteria-associated products; especially enzymes are gaining immense interest among worldwide researchers. Among several enzymes, amylases are of great significance in bioprocess engineering. This investigation was aimed to optimize the amylase production from Glutamicibacter arilaitensis strain ALA4 using goat dung as an inexpensive substrate in solid-state fermentation.Results: Amylase production was initially improved by optimizing physical factors and medium components by one factor at a time method. Two-level full factorial design (2 5 factorial matrix) was applied to screen the selected variables using first-order polynomial model. Parameters such as incubation temperature, moisture, starch, and yeast extract affected the amylase activity significantly (P < 0.05). Central composite design at N = 30 was further employed to evaluate the optimum levels of these variables by a second-order polynomial equation. Maximal amylase activity of 4572.53 ± 41.71 U/g was estimated in the goat dung medium supplemented with 100% moisture, 1% (w/w) starch, and 1% (w/w) yeast extract, being incubated at 40 °C. The optimized parameters revealed approximately twofold increment in the amylase yield (R 2 0.9169) with respect to the original medium. The amylase showed stability at high pH and temperature up to 4 h of incubation with residual activities of 52.32 ± 2.2 and 46.12 ± 3.3%, respectively. Additionally, the enzyme revealed resistant property not only towards various metal ions and organic solvents but also surfactants and inhibitors. Most importantly, the amylases obtained from strain ALA4 depicted remarkable tolerance to commercially available various detergents. Conclusions:This study reports first reference on the hyper-production of amylase from G. arilaitensis using goat dung as the low-cost agro-waste medium. G. arilaitensis strain ALA4 may be utilized for wide spread applications in several bioprocess industries due to the high stability of its amylase towards diversified pH, temperature, solvents, surfactants, and detergents.

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Section: Discussionsupporting

confidence: 89%

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

Aarti¹,

Khusro²,

Agastian³

2017

Bioresour. Bioprocess.

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“…Тому вивчення біогенезу H 2 S бактеріями важливе для розроблення ефективних і рентабельних біологічних способів регулювання рівня сполук сульфуру та металів у забруднених середовищах. Відбирання виділених із техногенно змінених екотопів, адаптованих до несприятливих умов штамів мікроорганізмів,особливо актуальне завдання для створення нових способів очищення довкілля (Wang et al, 2008;Zhuang et al, 2012;Iwahori et al, 2014;Limcharoensuk et al, 2015;Mustapha & Halimoon, 2015;Rabus et al, 2015;Si et al, 2015;Dey et al, 2016;Kiran et al, 2017). Мета статтівивчити вплив солей плюмбуму, купруму (ІІ), феруму (ІІ) та мангану (ІІ) на утворення гідроген сульфіду сульфат-і сірковідновними бактеріями, виділеними з води озера Яворівське, оцінити ефективність їх використання для очищення середовищ, збагачених органічними сполуками, від гідроген сульфіду та важких металів.…”

Section: вступunclassified

“…Ефективність мікробіологічного осадження іонів металів у вигляді МеS гідроген сульфідом, утвореним клітинами сульфат-і сірковідновних бактерій, залежить від концентрації гідроген сульфіду, який вони утворюють у процесі дисиміляційної сульфат-або сіркоредукції (Wang et al, 2008;Richter et al, 2012;Gudz et al, 2011;Moroz, 2013;Kuznetsov et al, 2015;Kiran et al, 2017). З іншого боку, інтенсивність анаеробного дихання мікроорганізмів у забруднених екотопах визначається рівнем їх адаптації до несприятливих умов довкілля (Zhuang et al, 2012;Iwahori et al, 2014;Viti et al, 2014;Limcharoensuk et al, 2015;Mustapha & Halimoon, 2015;Rabus et al, 2015;Si et al, 2015;Dey et al, 2016;Kiran et al, 2017). Штами сульфатвідновних бактерій Desulfovibrio desulfuricans ІМВ K-6, Desulfovibrio sp.…”

Section: обговоренняunclassified

Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds

et al. 2018

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Moroz, O. M., Hnatush, S. O., Tarabas, O. V., Bohoslavets, C. I., Yavorska, G. V., & Borsukevych, B. M. (2018). Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds. Biosystems Diversity, 26(1), 3-10.Due to their high content in natural environments, heavy metals exhibit toxic effects on living organisms, which leads to a decrease in the biological diversity and productivity of ecosystems. In niches with low oxidation reducing potential, sulfate and sulfur reducing bacteria carry out the reducing transformation of oxidized sulfur compounds with the formation of significant amounts of hydrogen sulfide. H 2 S produced by bacteria interacts with metal ions, precipitating them in the form of sulfides. The aim of this work was to investigate the influence of lead, cuprum (II), iron (II) and manganese (II) salts on the production of hydrogen sulfide by bacteria of the Desulfovibrio and Desulfuromonas genera, isolated from Yavorivske Lake, and to evaluate the efficiency of their use for purifying media, enriched with organic compounds, from hydrogen sulfide and heavy metals. The content of heavy metal ions in the water of Yavorivske Lake was determined by the spectrophotometric method. The bacteria were grown for 10 days at 30 °C in the Kravtsov-Sorokin medium under anaerobic conditions. To study the influence of metal ions on bacteria growth and their H 2 S production, cells were incubated with metal salts (0.5-4.0 mM), washed and grown in media with SO 4 2or S 0 . To determine the level of metal ions binding by H 2 S, produced by bacteria, cells were grown in media with metal compounds (0.5-4.0 mM), SO 4 2or S 0 . Biomass was determined by turbidimetric method. In the cultural liquid the content of H 2 S was determined quantitatively by spectrophotometric method, and qualitatively by the presence of metal cations. The content of metal sulfides in the growth medium was determined by weight method. Sulfate and sulfur-reducing bacteria were resistant to 2.0 mM Pb(NO 3 ) 2 , 2.5 mM CuCl 2 , 2.5 mM FeCl 2 × 4H 2 O and 2.0 mM MnCl 2 × 4H 2 O, therefore they are promising for the development of biotechnologies for the purification of water resources contaminated by sulfur and metal compounds. When present in a medium with sulfates or sulfur of 1.0-1.5 mM lead, cuprum (II), iron (II) or manganese (II) ions, they almost completely bind with the H 2 S produced by bacteria in the form of insoluble sulfides, which confirms the negative results of qualitative reactions to their presence in the cultural liquid.

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“…It has been reported that, arsenite and arsenate forms are poisonous to environment as well as human health (Dey et al 2016). The activity that leads to As pollution are mining, smelting, ore processing, and utilization of arsenic-based pesticides or herbicides, and arsenic contaminated water when irrigated posed heavy contamination of soil, especially in cultivated land ecosystem (Shagol et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Screening of Potent Arsenic Resistant and Plant Growth Promoting Bacillus species from the Soil of Terai Region of Nepal

Poudel¹,

Nepal²,

Magar³

et al. 2019

TU J. Microbiol.

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Objectives: To isolate arsenic resistant Bacillus spp. and to determine plant growth promoting activities. Methods: Eighteen soil samples were collected from the agricultural soil of Terai region of Nepal. Selective isolation of Bacillus species was done by heating the soil at 80 ºC for 15 minutes before the isolation. Nutrient agar was used as an isolation medium. Screening of arsenic resistant Bacillus species was done using nutrient agar supplemented with 100 ppm sodium arsenate and sodium arsenite. For plant growth promoting activity; IAA production was detected taking 0.1% tryptophane and measuring absorbance at 540 nm, NH3 production was tested by Nessler’s reagent and phosphate solubilization activity was detected by growing colonies on Pikovskaya’s agar. Sugar assimilation test was performed to identify the isolates. Most potent arsenic resistant isolate was identified by 16S rRNA gene sequencing. Results: Among 54 randomly selected isolates, 42 were found to be Gram-positive rod-shaped, spore-forming while 12 isolates were Gram-negative bacteria. The isolates IN12a, M12a and BG34a showed growth on 100 ppm sodium arsenite containing NA. Only isolate M12a tolerated up to 1000 ppm and 15000 ppm of sodium arsenite and sodium arsenate respectively, while other isolates could not grow above 400 ppm sodium arsenite. The isolates IN12a and M12a were able to produce IAA and solubilize phosphate while BG34a could not. Both the isolates IN12a and M12a were able to utilize the sugars glucose, fructose, lactose, sucrose, galactose, mannose, mannitol, maltose and xylose. Based on the 16S rRNA gene sequencing, isolate M12a was identified to be Bacillus flexus with highest similarity of 99.2%. Conclusion: Arsenic resistant and plant growth promoting Bacillus spp. was isolated from the agricultural soil of Terai region of Nepal

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Isolation and characterization of arsenic-resistant bacteria and possible application in bioremediation

Abstract: HighlightsIsolation of two rod-shaped Gram-positive bacteria.Isolates tolerate up to 4500 ppm and 550 ppm concentration of arsenate and arsenite.Bacteria mediated arsenic bioremediation.

Cited by 201 publications

References 33 publications

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds

Screening of Potent Arsenic Resistant and Plant Growth Promoting Bacillus species from the Soil of Terai Region of Nepal

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