Isolation and characterization of avian influenza viruses from raw poultry products illegally imported to Japan by international flight passengers

Shibata, Akihiro; Hiono, Takahiro; Fukuhara, Hisae; Sumiyoshi, Riho; Ohkawara, Ayako; Matsuno, Keita; Okamatsu, Masatoshi; Osaka, Hiroyuki; Sakoda, Yoshihiro

doi:10.1111/tbed.12726

Cited by 28 publications

(39 citation statements)

References 39 publications

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“… *: They are originated from Takemae et al., and Shibata et al., and sequences of VN334 were obtained in this study. …”

Section: Methodsmentioning

confidence: 95%

“…The H5N6 HPAIVs A/chicken/Niigata/1‐1T/2016 (Ck/Niigata), A/Muscovy duck/Aomori/1‐3T/2016 (MusDk/Aomori), A/chicken/Niigata/2‐5C/2016 (Ck/Niigata2), A/Muscovy duck/Aomori/2‐2T/2016 (MusDk/Aomori2), A/chicken/Hokkaido/1‐1C2C/2016 (Ck/Hokkaido), A/chicken/Miyazaki/1‐7T/2016 (Ck/Miyazaki), A/chicken/Kumamoto/1‐1T/2016 (Ck/Kumamoto1‐1T), A/chicken/Kumamoto/1‐2C/2016 (Ck/Kumamoto1‐2C), A/chicken/Gifu/1‐1T/2017 (Ck/Gifu), A/mute swan/Kyoto/1T/2016 (MuSw/Kyoto), A/chicken/Miyazaki/2‐2C/2017 (Ck/Miyazaki2), A/chicken/Saga/1‐1T/2017 (Ck/Saga), A/chicken/Chiba/1‐1T/2017 (Ck/Chiba), A/chicken/Miyagi/1‐5T/2017 (Ck/Miyagi) and A/duck/Hyogo/1/2016 (Dk/Hyogo) were isolated from poultry, captive birds or wild bird faeces in Japan between November 2016 and March 2017. Two H5N6 HPAIVs of foreign origin were also used in this study: A/duck/Quang Ngai/AI334/2014 (VN334; H5N6), which was isolated from an internal organ homogenate of a duck with suspected HPAI infection in Vietnam and A/chicken/Japan/AnimalQuarantine‐HE144/2016 (HE144; H5N6), which was isolated from raw chicken product imported from China (Shibata et al., ) were kindly provided by the Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries of Japan. These H5N6 HPAIVs were used for animal experiments, haemagglutination inhibition tests and temperature sensitivity assessments.…”

Section: Methodsmentioning

confidence: 99%

“…The sequences of the eight gene fragments were obtained by using the Automatic Influenza Genome Assembly and Subtyping system (FluGAS, World Fusion). The nucleotide sequences of the Japanese H5N6 HPAIVs and HE144 were published previously (Shibata et al., and Takemae et al., ) and were retrieved from the GISAID database. The accession numbers of the sequences used for the amino acid comparison are listed in Table .…”

Section: Methodsmentioning

confidence: 99%

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Comparative pathogenicity of H5N6 subtype highly pathogenic avian influenza viruses in chicken, Pekin duck and Muscovy duck

Uchida

Mine

Takemae

et al. 2019

Transbound Emerg Dis

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Summary In Japan during the 2016–2017 winter season, clade 2.3.4.4 highly pathogenic avian influenza viruses (HPAIVs) of the H5N6 subtype caused 12 outbreaks in chicken and Muscovy duck farms. These viruses have been circulating in Vietnam and China since 2014. In this study, we evaluated the susceptibility of chicken, Pekin duck (Anas platyrhynchos domesticus) and Muscovy duck (Cairina moschata) to H5N6 HPAIVs that originated in Japan, Vietnam and China. The H5N6 HPAIVs examined in this study were highly lethal to chickens compared with their pathogenicity in Pekin duck and Muscovy duck. One of five chickens infected with A/Muscovy duck/Aomori/1‐3T/2016 (MusDk/Aomori) survived despite viral shedding, although all of the chickens infected with the other viruses died. The 50% chicken lethal dose differed among the Japanese strains that shared the same gene constellation indicating that gene constellation was not a major determinant of pathogenicity in chicken. MusDk/Aomori, A/chicken/Niigata/1‐1T/2016 (Ck/Niigata) and A/duck/Hyogo/1/2016 (Dk/Hyogo) infected all Muscovy ducks inoculated; Ck/Niigata killed 50% of the ducks it infected whereas the other two did not kill any ducks. A/chicken/Japan/AnimalQuarantine‐HE144/2016 (HE144) isolated from chicken meat that originated in China was highly pathogenic to Pekin duck: all of the ducks died within 3.75 days of inoculation. This study shows that the pathogenicity of the clade 2.3.4.4 H5N6 HPAIVs differs not only between hosts but also within the same host species.

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“… *: They are originated from Takemae et al., and Shibata et al., and sequences of VN334 were obtained in this study. …”

Section: Methodsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Comparative pathogenicity of H5N6 subtype highly pathogenic avian influenza viruses in chicken, Pekin duck and Muscovy duck

Uchida

Mine

Takemae

et al. 2019

Transbound Emerg Dis

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show abstract

“…However, Slomka et al (2018) have detected systemic dissemination of the LPAIV H7N9 subtype to multiple organs beyond the respiratory and digestive tracts, including skeletal muscles and feather follicles in experimentally infected turkeys. Shibata et al (2018a) tested chicken, Pekin duck and Muscovy duck meat that had been illegally imported by international flight passengers and detected H5N1 HPAIV and H5N6 HPAIV as well as H9N2 LPAIV and H1N2 LPAIV. Uchida et al (2017) (0.44 log 10 EID 50 ) from the muscle tissue of quail (but not chickens or pigeons) inoculated with 6 log 10 EID 50 via the intranasal route.…”

Section: Assessment Of Lpaiv Transmission Via Raw Poultry Meatmentioning

confidence: 99%

“…Ejaz et al (2007) infected 10-week-old broiler chickens intranasally with LPAI H9N2 virus. The isolation of LPAI H7N9, H9N2 and LPAI H1N2 viruses from illegally imported chicken and Muscovy duck meat (Shibata et al, 2018a) indicates that LPAIV could persist during at least some steps of the food chain. On a weekly basis, samples were thawed, and the presence of the virus was checked through embryonated egg inoculation.…”

Section: 4mentioning

confidence: 99%

Assessment of low pathogenic avian influenza virus transmission via raw poultry meat and raw table eggs

Gonzáles¹,

Roberts²,

Śmietanka³

et al. 2018

EFS2

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A rapid qualitative assessment has been done by performing a theoretical analysis on the transmission of low pathogenic avian influenza (LPAI) via fresh meat from poultry reared or kept in captivity for the production of meat (raw poultry meat) or raw table eggs. A predetermined transmission pathway followed a number of steps from a commercial or non‐commercial poultry establishment within the EU exposed to LPAI virus (LPAIV) to the onward virus transmission to animals and humans. The combined probability of exposure and subsequent LPAIV infection via raw poultry meat containing LPAIV is negligible for commercial poultry and humans exposed via consumption whereas it is very unlikely for non‐commercial poultry, wild birds and humans exposed via handling and manipulation. The probability of LPAIV transmission from an individual infected via raw poultry meat containing LPAIV is negligible for commercial poultry and humans, whereas it is very unlikely for non‐commercial poultry and wild birds. The combined probability of exposure and subsequent LPAIV infection via raw table eggs containing LPAIV is negligible for commercial poultry and humans and extremely unlikely to negligible for non‐commercial poultry and wild birds. The probability of LPAIV transmission from an individual infected via raw table eggs containing LPAIV is negligible for commercial poultry and humans and very unlikely to negligible for non‐commercial poultry and wild birds. Although the presence of LPAIV in raw poultry meat and table eggs is very unlikely to negligible, there is in general a high level of uncertainty on the estimation of the subsequent probabilities of key steps of the transmission pathways for poultry and wild birds, mainly due to the limited number of studies available, for instance on the viral load required to infect a bird via raw poultry meat or raw table eggs containing LPAIV.

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Application of an Improved Micro-amount of Virion Enrichment Technique (MiVET) for the Detection of Avian Influenza A Virus in Spiked Chicken Meat Samples

et al. 2020

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Highly sensitive detection of pathogens is effective for screening meat during quarantine inspection and export. The "micro-amount of virion enrichment technique" (MiVET) was recently developed, which is a new method combining virus concentration with immunomagnetic beads and simple RNA extraction with sodium dodecyl benzenesulfonate (SDBS) for the specific and sensitive detection of avian influenza viruses (AIVs). AIV subtypes H3N2 and H4N2 were used to spike the surface of chicken breast meat samples. The modified MiVET protocol was tested by comparing it against three different homogenate preparation conditions, as well as in samples with added α-amylase and collagenase to digest inhibitors. The performance of the modified MiVET was evaluated by real-time RT-PCR assay targeting the matrix gene. Compared with conventional RNA extraction, the modified MiVET reproducibly concentrated AIVs in chicken meat samples with 100-1000-fold improvement by 60 s-hand homogenization. The 30 s-and 60 s-4 2 1 2 3 4stomacher homogenizations resulted 100-fold and 10-100-fold improvement, respectively. The modified MiVET required < 60 min from homogenate preparation to final RNA elution. Further, use of the modified MiVET also decreased the rate of false-negative results. The modified MiVET is effective for the rapid and highly sensitive detection of AIVs in chicken meat samples, and can be applied to quarantine and export inspection at airports and seaports.

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Isolation and characterization of avian influenza viruses from raw poultry products illegally imported to Japan by international flight passengers

Cited by 28 publications

References 39 publications

Comparative pathogenicity of H5N6 subtype highly pathogenic avian influenza viruses in chicken, Pekin duck and Muscovy duck

Comparative pathogenicity of H5N6 subtype highly pathogenic avian influenza viruses in chicken, Pekin duck and Muscovy duck

Assessment of low pathogenic avian influenza virus transmission via raw poultry meat and raw table eggs

Application of an Improved Micro-amount of Virion Enrichment Technique (MiVET) for the Detection of Avian Influenza A Virus in Spiked Chicken Meat Samples

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