Isolation and characterization of biosurfactant-producing and diesel oil degrading<i>Pseudomonas</i>sp. CQ2 from Changqing oil field, China

Sun, Wanqing; Cao, Wen-Rui; Jiang, Mingyu; Saren, Gaowa; Liu, Jiwei; Cao, Jiangfei; Ali, Imran; Yu, Xinke; Peng, Changsheng; Naz, Iffat

doi:10.1039/c8ra07721e

Cited by 35 publications

(27 citation statements)

References 66 publications

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“…This finding suggests the biosurfactant production ability by these examined isolates. In addition, the current value of surface tension was higher than that observed by Ahmad et al, (2016), Sun et al, (2018) and Ibrahim, (2018). 5,14,25 They found a decrease in surface tension to less than 40 mN/m by different tested biosurfactant producing isolates.…”

Section: Original Effect Of Nutritional Factors and Growth Conditions...contrasting

confidence: 59%

Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Motwali,

Aly,

Qari

et al. 2021

J. Contemp. Med. Sci.

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Objectives:The current study aimed to investigate the impact of different nutritional factors and growth conditions on biosurfactant production by bacterial isolates (EMB16 and EMB21) isolated from oil-contaminated soil samples. Methods: Based on the used quantitative and qualitative screening method in current study, the selected bacterial isolates showed a high potential to produce biosurfactant. Results: By using 16S rRNA sequence analysis, the bacterial isolates EMB16 and EMB21 were found to be closely related to Pseudomonas mendocina and Pseudomonas oleovorans, respectively. The ultimate yields of biosurfactant (8.06 ± 0.06 mg/ml) by Ps. mendocina EMB16 was with corn oil as a carbon source, urea as a nitrogen source, C/N ratio of 30, pH value of 7, and 2% inoculum size. For Ps. oleovorans EMB21, the maximum biosurfactant production (4.68 ± 0.14 mg/ml) was achieved by diesel oil as a carbon source, urea as a nitrogen source, C/N ratio of 30, pH of 7, and 5% size of inoculum. The best incubation period and temperature for the examined strains was 168 hrs. at 37°C. Conclusion:The results proved that Ps. mendocina EMB16 was the most efficient biosurfactant producer as it showed the greatest amount of biosurfactant concentration and lowest value of surface tension measurement with an emulsification index of 67 ± 6%.

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Section: Original Effect Of Nutritional Factors and Growth Conditions...contrasting

confidence: 59%

Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Motwali,

Aly,

Qari

et al. 2021

J. Contemp. Med. Sci.

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“…Petroleum contains many toxic and harmful components with negative impact on the ecological environment and the use of biological methods, as microorganisms, could be an excellent alternative as compared with physical and chemical remediation techniques in addition to produce a bioactive compound as biosurfactants (Sun et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Production of biosurfactants by bacteria collected in soil contaminated by diesel oil on the Antarctic Continent

Machado

Cordeiro²,

Oliveira³

et al. 2022

RSD

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Currently, the whole world strives for a sustainable development to avoid the depletion of natural resources. Therefore, the search for new biotechnological products such as biosurfactants has generated interest due to its low toxicity, biodegradability and ecological acceptability. Furthermore, biosurfactants have several applications such as emulsification, wettability, foaming, cleaning, and reduction of crude oil viscosity. The objective of this work was to identify the bacteria collected in Antarctica and evaluate their capacity to produce biosurfactants. In this study, bacteria collected in diesel contaminated soil around the Comandante Ferraz Antarctic Station were identified (Rhodococcus erythropolis, Rhizobium radiobacter, Microbacterium liquefacien, Pseudomonas libanensis and Pseudomonas veronii) and analyses were carried out to confirm whether these bacteria produce biosurfactants. Interestingly, the compounds isolated from the bacteria showed droplet propagation and reduced the surface tension of the water, which are properties of biosurfactants. Furthermore, these compounds showed carbohydrates in their molecular composition. Given these findings, these species have potential application in biotechnological areas, such as bioremediation of environments contaminated by diesel oil and in cosmetic and personal care as emulsifier. In addition, the use of diesel oil waste by bacteria as a carbon source becomes an attractive alternative because it reduces environmental contamination and has a low cost.

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“…The biosurfactant was extracted using the method of Sun et al (2018) . After 96 h of incubation, the bacterial suspension was centrifuged (8,000 g ) at 4°C for 20 min.…”

Section: Methodsmentioning

confidence: 99%

Formulation of a Culture Medium to Optimize the Production of Lipopeptide Biosurfactant by a New Isolate of Bacillus sp.: A Soil Heavy Metal Mitigation Approach

et al. 2022

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This research aimed to optimize a lipopeptide biosurfactant produced from Bacillus sp. SHA302 due to its high efficiency of heavy metal release in soil. The results demonstrated that the metal release capacity of the lipopeptide biosurfactant alone increased with increasing the biosurfactant concentration. Among treatments with different biosurfactant concentrations plus acid, the highest metal release rates of 53.8% ± 1.4 and 39.3% ± 1.7 for Zn and Pb, respectively, were observed in the critical micelle concentration (CMC) + HCl treatment. The results of a factorial experiment designed for optimizing biosurfactant production showed that among five inexpensive carbon sources and six mineral nitrogen sources, sugar beet molasses (1%) and ammonium chloride (0.1%) were the most efficient sources in lowering the surface tension (ST) of the culture media to 32.2 ± 0.76 mN/m. The second step of the experiment was a Plackett–Burman design with 11 factors and showed that the four factors of pH, ammonium chloride, magnesium sulfate, and molasses significantly affected (P < 0.05) the changes in ST and biosurfactant production. The third step of the experiment was done using the response surface methodology (RSM) with a central composite design. The results showed that a pH of 7.3, 1.5 g/l of ammonium chloride, 0.3 g/l of magnesium sulfate, and 10% of sugar beet molasses yielded values of 29.2 ± 0.71 mN/m and 5.74 ± 0.52 g/l for the two variables of ST and biosurfactant production, respectively, which reached their most optimal levels.

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Isolation and characterization of biosurfactant-producing and diesel oil degradingPseudomonassp. CQ2 from Changqing oil field, China

Abstract: In the present research investigation, 13 indigenous bacteria (from CQ1 to CQ13) were isolated from soil collected from Changqing oil field of Xi'an, China.

Cited by 35 publications

References 66 publications

Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Production of biosurfactants by bacteria collected in soil contaminated by diesel oil on the Antarctic Continent

Formulation of a Culture Medium to Optimize the Production of Lipopeptide Biosurfactant by a New Isolate of Bacillus sp.: A Soil Heavy Metal Mitigation Approach

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