Isolation and characterization of brown algal polyphenols as inhibitors of α-amylase, lipase and trypsin

Barwell, C. J.; Blunden, Gerald; Manandhar, Prabha D.

doi:10.1007/bf00003468

Cited by 30 publications

(24 citation statements)

References 5 publications

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“…Our results confirmed what was reported by other authors who found that green, red, and brown algae exhibited inhibitor activities against lipases [23,24]. However, it is very important to note that variations in the inhibitory capacities of algal extracts could be related to many factors (the species, the biological stage, environmental conditions, the extraction processing conditions, etc.).…”

Section: Discussionsupporting

confidence: 92%

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Inhibitory Effects of Tunisian Marine Algal Extracts on Digestive Lipases

Rebah

Smaoui²,

Frikha³

et al. 2008

Appl Biochem Biotechnol

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The lipase inhibitory activity of ethanol extracts obtained from some marine algae collected on the Tunisian coast was evaluated. Caulerpa prolifera extract markedly reduced both dog gastric (DGL) and human pancreatic lipase (HPL) activities. Generally, the inhibition reached 100% after 40 to 60 min of incubation depending on lipase types and on substrates used. Moreover, the inhibitory effect of C. prolifera extract on lipases appeared to be accelerated by adding bile salts, which likely modified the interface and allowed the inhibitory compound to inactivate the lipase. The separation of C. prolifera extract by thin-layer chromatography (TLC) resulted in eight fractions showing efficient inhibition rate against DGL, compared to the crude extract. In the case of HPL, TLC fractionation reduced the inhibitory rates, suggesting that the effect of algal extract on lipases may be caused by a synergetic action of several compounds within the extract. High-performance liquid chromatograph separation resulted in isolation of a major compound displaying high inhibition capacity of HPL activity. Caulerpa prolifera extract may therefore be useful in developing antiobesity drugs.

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Section: Discussionsupporting

confidence: 92%

“…In addition, it has been shown in vitro that some condensed polyphenols, such as tannin, had an inhibitory action on different digestive enzymes such as proteases, alfa-amylase, and pancreatic lipase [24]. Our findings suggested that C. prolifera may prove to be a good source of effective crude drug for the treatment of obesity.…”

Section: Discussionmentioning

confidence: 55%

Inhibitory Effects of Tunisian Marine Algal Extracts on Digestive Lipases

Rebah

Smaoui²,

Frikha³

et al. 2008

Appl Biochem Biotechnol

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“…Phlorotannins are produced by polymerization of phloroglucinol and are known only from brown algae; in addition to UV protection, they have a role in discouraging grazing (135) and inhibit enzyme activity and potentially digestion (7). Tannins present in vascular plants (102) have similar properties, and their occurrence in vascular plants correlates with expanded numbers of mammalian grazers (105).…”

Section: Streptophytinamentioning

confidence: 99%

Evolution and Diversity of Plant Cell Walls: From Algae to Flowering Plants

Popper

Michel

Hervé

et al. 2011

Annu. Rev. Plant Biol.

620

483

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All photosynthetic multicellular Eukaryotes, including land plants and algae, have cells that are surrounded by a dynamic, complex, carbohydrate-rich cell wall. The cell wall exerts considerable biological and biomechanical control over individual cells and organisms, thus playing a key role in their environmental interactions. This has resulted in compositional variation that is dependent on developmental stage, cell type, and season. Further variation is evident that has a phylogenetic basis. Plants and algae have a complex phylogenetic history, including acquisition of genes responsible for carbohydrate synthesis and modification through a series of primary (leading to red algae, green algae, and land plants) and secondary (generating brown algae, diatoms, and dinoflagellates) endosymbiotic events. Therefore, organisms that have the shared features of photosynthesis and possession of a cell wall do not form a monophyletic group. Yet they contain some common wall components that can be explained increasingly by genetic and biochemical evidence. 567

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“…Polyphenolic content in solvent extract of A. nodosum was 82% of the total of untreated A. nodosum (data not shown), resulting in a further 5% being removed during enzymatic saccharification. Seaweeds with high polyphenolic content, such as members of the Fucales, have shown to inhibit microbial processes [67] largely due to the non-selective inactivation of enzymes [68]. Polyphenolics are also known to exhibit an affinity towards proteins but the effect of complex formation on the digestibility of proteins/phenolics and the activity of enzymes is still not fully understood [69] and both beneficial and detrimental effects of polyphenols on digestive enzymes have been reported [70].…”

Section: Polyphenol Contentmentioning

confidence: 99%

The nutritional aspects of biorefined Saccharina latissima, Ascophyllum nodosum and Palmaria palmata

Schiener

SuFen

Theodoridou

et al. 2016

Biomass Conv. Bioref.

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The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that: • a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders. Please consult the full DRO policy for further details. Abstract The chemical profile of biorefined Saccharina latissima, Ascophylum nodosum and Palmaria palmata after carbohydrate and polyphenol extraction was analysed with the aim to evaluate the nutritional aspects of biorefined seaweeds as a novel animal feed supplement. Optimised enzymatic saccharification has been used to show that the protein concentration in the residue of Palmaria palmata and Ascophylum nodosum can be increased by more than two fold. Nutritional value of the residue was further enhanced through an increase in total amino acids and fatty acids. As a consequence of removal of inorganic elements such as sodium, potassium and chloride, the total solid and ash content of all three seaweeds was reduced by around 40%. In contrast, divalent metals such as iron and zinc, as well as silicon accumulated in all three residues. Potentially harmful components such as arsenic and iodine were reduced only in brown biorefined seaweeds, whilst in biorefined P. palmata iodine increased by 39% compared to a 24% decline of arsenic. Polyphenol removal in all three seaweeds was >80% and, in combination with enzymatic saccharification, enhanced protein recovery in A. nodosum. This highlights the potential of biorefinery concepts to generate multiple products from seaweed such as extracts enriched in polyphenols and carbohydrates and residue with higher protein and lipid content.

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Isolation and characterization of brown algal polyphenols as inhibitors of α-amylase, lipase and trypsin

Cited by 30 publications

References 5 publications

Inhibitory Effects of Tunisian Marine Algal Extracts on Digestive Lipases

Inhibitory Effects of Tunisian Marine Algal Extracts on Digestive Lipases

Evolution and Diversity of Plant Cell Walls: From Algae to Flowering Plants

The nutritional aspects of biorefined Saccharina latissima, Ascophyllum nodosum and Palmaria palmata

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