Inhibitory Effects of Tunisian Marine Algal Extracts on Digestive Lipases

Abstract

The lipase inhibitory activity of ethanol extracts obtained from some marine algae collected on the Tunisian coast was evaluated. Caulerpa prolifera extract markedly reduced both dog gastric (DGL) and human pancreatic lipase (HPL) activities. Generally, the inhibition reached 100% after 40 to 60 min of incubation depending on lipase types and on substrates used. Moreover, the inhibitory effect of C. prolifera extract on lipases appeared to be accelerated by adding bile salts, which likely modified the interface and allowed the inhibitory compound to inactivate the lipase. The separation of C. prolifera extract by thin-layer chromatography (TLC) resulted in eight fractions showing efficient inhibition rate against DGL, compared to the crude extract. In the case of HPL, TLC fractionation reduced the inhibitory rates, suggesting that the effect of algal extract on lipases may be caused by a synergetic action of several compounds within the extract. High-performance liquid chromatograph separation resulted in isolation of a major compound displaying high inhibition capacity of HPL activity. Caulerpa prolifera extract may therefore be useful in developing antiobesity drugs.

Alpha-amylase inhibition kinetics by caulerpenyne

“…As a result of its unique properties, such as antiproliferative and apoptotic effects against some neuroblastoma, tumor and cancer cell lines (CAVAS et al, 2006;FISCHEL et al, 1995;BARBIER et al, 2001) and antiviral properties (NICOLETTI et al, 1999), LOX inhibition (NINOMIYA et al, 1998), PLA2 inhibition (MAYER et al, 1993, lipase inhibition (BITOU et al, 1999;REBAH et al, 2008) and alpha-amylase inhibition (TEIXEIRA et al, 2007) a great deal of research has been done. The CYN content in C. prolifera was found to be 6.34 mg/g wet weight of algae by using an HPLC method.…”

“…It has been reported that the methanolic extract of C. taxifolia and CYN inhibit pancreatic lipase activity (BITOU et al, 1999). The inhibition of lipase activity was also shown by the ethanolic extract of C. prolifera (REBAH et al, 2008). The inhibitory activity of CYN purified from C. prolifera was revealed by MAYER et al (1993).…”

Inhibition of xanthine oxidase by Caulerpenyne from Caulerpa prolifera

“…It was presented in the previous reports that CYN, the main secondary metabolite of Caulerpa species, has some unique properties such as anticarcinogenic, antiproliferative, apoptotic and antiviral effects [16][17][18][19]. Moreover, the enzyme inhibitory effects of CYN towards some medically important enzymes like phospholipase A 2 , lipase, lipoxygenase and amylase have been shown by some researchers [21][22][23][24][25][26][27]. In the light of the knowledge above, the inhibitory effects of CYN on XOD enzyme activity were determined in this study.…”

“…Due to the results of this study CYN can be classified as a good inhibitor for XOD and therefore, it can be recommended as a potential drug for XOD related diseases after in vivo studies. Unfortunately, CYN inhibits not only XOD but also phospholipase A 2 [21], lipoxygenase [22,23], pancreatic lipase [24,25] and alpha amylase [26,27]. Many drugs, which are currently used, act similarly and by reacting with different pathways they may have severe side effects.…”

“…There are few studies in the literature which evaluate the enzyme inhibitory potential of CYN and its derivatives. It inhibits phospholipase A 2 [21], lipoxygenase [22,23], pancreatic lipase [24,25] and α-amylase [26,27].…”