Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

Akita, Hironaga; Kimura, Zen‐ichiro; Yusoff, Mohd Zulkhairi Mohd; Nakashima, Nariyasu; Hashizume, Tamotsu

doi:10.1186/s40064-016-2237-y

Cited by 24 publications

(27 citation statements)

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“…Burkholderia, a proteobacteria belonging to class betaproteobacteria, order Burkholderiales and family Burkholderiaceae, is widely known for the biodegradation of diverse types of substrates and other biotechnological applications [17–21]. Jung et al used Burkholderia glumae BGR1 for the production of p -hydroxybenzoic acid from p -coumaric acid [18].…”

Section: Introductionmentioning

confidence: 99%

Genomic analysis of Burkholderia sp. ISTR5 for biofunneling of lignin-derived compounds

Morya

Kumar

Singh

et al. 2019

Biotechnol Biofuels

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BackgroundLignin is the second most abundant natural polymer on earth. Industries using lignocellulosic biomass as feedstock generate a considerable amount of lignin as a byproduct with minimal usage. For a sustainable biorefinery, the lignin must be utilized in improved ways. Lignin is recalcitrant to degradation due to the complex and heterogeneous structure. The depolymerization of lignin and its conversion into specific product stream are the major challenges associated with lignin valorization. The blend of oligomeric, dimeric and monomeric lignin-derived compounds (LDCs) generated during depolymerization can be utilized by microbes for production of bioproducts.ResultsIn the present study, a novel bacterium Burkholderia sp. strain ISTR5 (R5), a proteobacteria belonging to class betaproteobacteria, order Burkholderiales and family Burkholderiaceae, was isolated and characterized for the degradation of LDCs. R5 strain was cultured on 12 LDCs in mineral salt medium (MSM) supplemented with individual compounds such as syringic acid, p-coumaric acid, ferulic acid, vanillin, vanillic acid, guaiacol, 4-hydroxybenzoic acid, gallic acid, benzoic acid, syringaldehyde, veratryl alcohol and catechol. R5 was able to grow and utilize all the selected LDCs. The degradation of selected LDCs was monitored by bacterial growth, total organic carbon (TOC) removal and UV–Vis absorption spectra in scan mode. TOC reduction shown in the sample contains syringic acid 80.7%, ferulic acid 84.1%, p-coumaric acid 85.9% and benzoic acid 83.2%. In UV–Vis absorption spectral scan, most of the lignin-associated peaks were found at or near 280 nm wavelength in the obtained absorption spectra. Enzyme assay for the ligninolytic enzymes was also performed, and it was observed that lignin peroxidase and laccase were predominantly expressed. Furthermore, the GC–MS analysis of LDCs was performed to identify the degradation intermediates from these compounds. The genomic analysis showed the robustness of this strain and identified various candidate genes responsible for the degradation of aromatic or lignin derivatives, detoxification mechanism, oxidative stress response and fatty acid synthesis. The presence of peroxidases (13%), laccases (4%), monooxygenases (23%), dioxygenase (44%), NADPH: quinone oxidoreductases (16%) and many other related enzymes supported the degradation of LDCs.ConclusionNumerous pathway intermediates were observed during experiment. Vanillin was found during growth on syringic acid, ferulic acid and p-coumaric acid. Some other intermediates like catechol, acetovanillone, syringaldehyde and 3,4-dihydroxybenzaldehyde from the recognized bacterial metabolic pathways existed during growth on the LDCs. The ortho- and meta cleavage pathway enzymes, such as the catechol-1,2-dioxygenase, protocatechuate 3,4-dioxygenase, catechol-2,3-dioxygenase and toluene-2,3-dioxygenase, were observed in the genome. In addition to the common aromatic degradation pathways, presence of the epoxyqueuosine reductase, 1,2-epoxyphenylacetyl-CoA isomer...

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Section: Introductionmentioning

confidence: 99%

Genomic analysis of Burkholderia sp. ISTR5 for biofunneling of lignin-derived compounds

Morya

Kumar

Singh

et al. 2019

Biotechnol Biofuels

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“…(DSM 104486)Mature vegetable compostEnrich bacteria in mineral salts medium with kraft pulping stream or technical kraft lignin as the sole carbon sourceCan catabolize vanillin, vanillic acid, 4-hydroxybenzic acid, p -coumaric acid, benzoic acid, and ferulic acid[141] P. putida NX-1Leaf mold samplesEnrich bacteria in M9 medium supplemented with kraft ligninPossess high lignin peroxidase activity, manganese peroxidase activity and laccase activity[28] Burkholderia sp. strain CCA53Soil samples from Higashi-HiroshimaEnrich bacteria in mineral salts medium with alkali lignin as the sole carbon sourceCan catabolize benzaldehyde, benzoic acid, catechol, 4-hydroxy benzaldehyde, 4-hydroxy benzoic acid, 4-hydroxybenzyl alcohol, syringol, and vanillin[142] Serratia sp. JHT01 (DSM 29580), Serratia liquefaciens sp.…”

Section: The Lignin-degrading Bacteria and Their Screening Methodsmentioning

confidence: 99%

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products

Lei²,

Zhai

et al. 2019

Biotechnol Biofuels

211

119

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Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorization of lignin to chemicals and fuels. However, due to the structural heterogeneity of lignin, depolymerization and fragmentation typically result in diverse product species, which require extensive separation and purification procedures to obtain target products. For lignin valorization, bacterial-based systems have attracted increasing attention because of their diverse metabolisms, which can be used to funnel multiple lignin-based compounds into specific target products. Here, recent advances in lignin valorization using bacteria are critically reviewed, including lignin-degrading bacteria that are able to degrade lignin and use lignin-associated aromatics, various associated metabolic pathways, and application of bacterial cultures for lignin valorization. This review will provide insight into the recent breakthroughs and future trends of lignin valorization based on bacterial systems.

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“…Burkholderia sp. strain CCA53 was recently isolated from leaf soil and classified into group B ( 3 ). An important limitation of industrial host microorganisms, such as Escherichia coli and Saccharomyces cerevisiae , is an inability to assimilate lignin as a carbon source, but Burkholderia sp.…”

Section: Genome Announcementmentioning

confidence: 99%

“…An important limitation of industrial host microorganisms, such as Escherichia coli and Saccharomyces cerevisiae , is an inability to assimilate lignin as a carbon source, but Burkholderia sp. CCA53 has this ability ( 3 ). Because of that, it is anticipated that Burkholderia sp.…”

Section: Genome Announcementmentioning

confidence: 99%

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Draft Genome Sequence of Burkholderia sp. Strain CCA53, Isolated from Leaf Soil

et al. 2016

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Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

Cited by 24 publications

References 23 publications

Genomic analysis of Burkholderia sp. ISTR5 for biofunneling of lignin-derived compounds

Genomic analysis of Burkholderia sp. ISTR5 for biofunneling of lignin-derived compounds

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products

Draft Genome Sequence of Burkholderia sp. Strain CCA53, Isolated from Leaf Soil

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