Isolation and characterization of cancer stem like cells in human glioblastoma cell lines

Qiang, Lei; Yang, Yong; Ma, Yanju; Chen, Feihong; Zhang, Ling-Bo; Liu, Wei; Qi, Qi; Lu, Na; Tao, Lei; Wang, Xiaotang; You, Qidong; Guo, Qinglong

doi:10.1016/j.canlet.2009.01.016

Cited by 178 publications

(147 citation statements)

References 23 publications

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“…And interestingly, we noted that hypoxia promotes the expansion of CD133-positive cells. 9 Here, we provide evidence that HIF-1a, which is regulated by several signal pathways, including STAT3, PI3K-Akt, and ERK1/2 at different stage, is essential for hypoxia-mediated maintenance of GSC by activating Notch signaling pathway.…”

mentioning

confidence: 67%

“…Many studies have reported CD133-positive cells and SP cells enrich the GSC. 9,23 Flow cytometric analysis indicated that the percentage of CD133 þ cells and SP cells increased (Figures 1d and e) after these glioblastoma cells were exposed to hypoxia. Specifically, the percentage of CD133 þ cells obviously increased in hypoxiatreated U251 cells (Figure 1d).…”

Section: Resultsmentioning

confidence: 98%

“…9,36,37 These studies suggest that tumor sphere cells maybe a convenient and useful model for cancer stem cells research, although not every cell within tumor spheres is cancer stem cell. Here, we take U251SC, a U251 derived tumor sphere cells, as a GSC model.…”

Section: Discussionmentioning

confidence: 99%

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HIF-1α is critical for hypoxia-mediated maintenance of glioblastoma stem cells by activating Notch signaling pathway

et al. 2011

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mentioning

confidence: 67%

Section: Resultsmentioning

confidence: 98%

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HIF-1α is critical for hypoxia-mediated maintenance of glioblastoma stem cells by activating Notch signaling pathway

et al. 2011

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“…The concept of cancer stem cells (CSCs) brought a change of paradigm in cancer biology and therapeutics [2,3], suggesting that specifically targeting these cells for destruction [4] or differentiation [5] may be the best therapeutic strategy to follow for several aggressive types of cancers. This, however, may not be easy, since CSCs have shown to present less reactive oxygen species [6] and to be more resistant to ionizing radiation [7], vincristine [8], hypoxia, and other chemotherapeutics [9] when compared to nonCSCs. Treatment with some of these agents increased the proportion of CSCs, which may be an explanation for more aggressive recurrence [7].…”

Section: Introductionmentioning

confidence: 99%

Extracellular ATP reduces tumor sphere growth and cancer stem cell population in glioblastoma cells

Ledur

Villodre

Paulus

et al. 2011

Purinergic Signalling

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Glioblastoma is the most aggressive tumor in the CNS and is characterized by having a cancer stem cell (CSC) subpopulation essential for tumor survival. The purinergic system plays an important role in glioma growth, since adenosine triphosphate (ATP) can induce proliferation of glioma cells, and alteration in extracellular ATP degradation by the use of exogenous nucleotidases dramatically alters the size of gliomas in rats. The aim of this work was to characterize the effect of the purinergic system on glioma CSCs. Human U87 glioma cultures presented tumor spheres that express the markers of glioma cancer stem cells CD133, Oct-4, and Nanog. Messenger RNA of several purinergic receptors were differently expressed in spheres when compared to a cell monolayer not containing spheres. Treatment of human gliomas U87 or U343 as well as rat C6 gliomas with 100 μM of ATP reduced the number of tumor spheres when grown in neural stem cell medium supplemented with epidermal growth factor and basic fibroblast growth factor. Moreover, ATP caused a decline in the number of spheres observed in culture in a dose-dependent manner. ATP also reduces the expression of Nanog, as determined by flow cytometry, as well as CD133 and Oct-4, as analyzed by flow cytometry and RT-PCR in U87 cells. The differential expression of purinergic receptor in tumor spheres when compared to adherent cells and the effect of ATP in reducing tumor spheres suggest that the purinergic system affects CSC biology and that ATP may be a potential agonist for differentiation therapy.

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“…In this study, phylligenin exhibited growthinhibitory effect to C6 glioma and U87MG glioblastoma cells lines. C6 and U87MG cells are reported to contain stem-like cell population (Qiang et al, 2009;Zhou et al, 2009). These results implicate that phylligenin have a potential to be developed for anti-cancer drugs presumably through BTSC inhibition.…”

Section: Discussionmentioning

confidence: 76%

Inhibitory Effects of Phylligenin on the Proliferation of Cultured Rat Neural Progenitor Cells

Lee¹,

Go²,

Choi³

et al. 2010

Biomolecules and Therapeutics

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-Neural progenitor cells (NPCs) differentiate into astrocytes, neurons and oligodendrocytes, which is controlled by various factors in brain. Recent evidences suggest that small molecules modulating the proliferation and differentiation of NPCs may have therapeutic value as well as the potential use as chemical probes. Phylligenin is a lignan with anti-inflammatory activity that is isolated from the fruits of Forsythia koreana. We investigated effects of phylligenin on proliferation and differentiation of NPCs. Treatment of phylligenin decreased the number of proliferating NPCs in culture without effects on the differentiation and survival of neural cells such as neurons and astrocytes. To examine the mechanism of the decreased NPCs number, we performed cell cycle analysis. Proliferation of NPCs was decreased via G1-S transition block by phylligenin treatment, and it was mediated by the increase of p21 level. However, phylligenin did not induce apoptosis of NPCs as determined by TUNEL assay and PARP cleavage. We also found that viability of glioma cell lines such as C6 and U87MG glioma cells, but not that of primary neuron and astrocyte, was inhibited by phylligenin. These results suggest that phylligenin selectively inhibits proliferation of rapidly growing cells such as neural stem cells and glioma cells. Given that the possible role of brain tumor stem cells in the pathology of brain cancers, the inhibitory effects of phylligenin might be useful in the development of new therapeutic agents against brain cancers.

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Isolation and characterization of cancer stem like cells in human glioblastoma cell lines

Cited by 178 publications

References 23 publications

HIF-1α is critical for hypoxia-mediated maintenance of glioblastoma stem cells by activating Notch signaling pathway

HIF-1α is critical for hypoxia-mediated maintenance of glioblastoma stem cells by activating Notch signaling pathway

Extracellular ATP reduces tumor sphere growth and cancer stem cell population in glioblastoma cells

Inhibitory Effects of Phylligenin on the Proliferation of Cultured Rat Neural Progenitor Cells

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