Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids

Sukma, Dewi; Handini, Aline Sisi; Sudarsono, Sudarsono

doi:10.13057/biodiv/d211109

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…As a result, this provides a novel approach to enhancing silymarin production (Wang et al 2017). CHS genes in numerous plant species were found and characterized (Yin et al 2019;Roslan et al 2020;Sukma et al 2020;Zhu et al 2022). In addition to genetic alterations, the generation of the corresponding secondary metabolites requires molecules that activate production.…”

Section: Chalcone Synthase-coding Chs Genementioning

confidence: 99%

Understanding of genes encoding bioactive compounds from potential medicinal plants in Indonesia as cancer cell inhibitors

MUFLIKHATI,

SIANIPAR,

SYAMSUNARNO

et al. 2023

Biodiversitas

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Abstract. Muflikhati Z, Sianipar NF, Syamsunarno MRAA, Anas. 2023. Understanding of genes encoding bioactive compounds from potential medicinal plants in indonesia as cancer cell inhibitors. Biodiversitas 24: 4645-4660. Indonesia has abundant plant diversity and enormous potential to be developed as a source of medicinal plants due to the content of bioactive chemicals in them. Potential medicinal plants from Indonesia, such as Andrographis paniculata (Sambiloto), Curcuma longa (Kunyit), Moringa oleifera (Moringa), Phyllanthus niruri (Meniran), Orthosiphon stamineus (Kumis kucing), Typhonium flagelliforme (Keladi tikus), and Zingiber officinale (Jahe), contain bioactive compounds with mechanisms that act as anticancer. Bioactive compounds with anticancer mechanisms in plants can be controlled by specific genes. Therefore, it is important to elaborate on bioactive compounds and the genes involved. This article aims to discuss the genes encoding bioactive compounds in Indonesian medicinal plants and the mechanisms that can inhibit cancer cell growth. Several genes encode anticancer compounds, such as lectin-coding genes, the stigmasterol-coding CYP710A gene, the tocopherol-coding ?-TMT gene, the fatty acid-coding FAD gene, the doxorubicin-coding DXR gene, and the chalcone synthase-coding CHS gene. These genes have mechanisms to increase the expression of several apoptosis-promoting proteins, including BCL-2 family members, in several cancer cell models. This article also describes the potential utilization and creation of molecular markers linked to genes encoding anticancer chemicals and can be used as a reference for research on medicinal plants that is still limited. Knowledge of genes encoding anticancer compounds in plants can support future research in the development of cancer drugs.

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Section: Chalcone Synthase-coding Chs Genementioning

confidence: 99%

Understanding of genes encoding bioactive compounds from potential medicinal plants in Indonesia as cancer cell inhibitors

MUFLIKHATI,

SIANIPAR,

SYAMSUNARNO

et al. 2023

Biodiversitas

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show abstract

“…Nucleotide mutation pattern analyses were carried out with the median-joining algorithm. 1 by using the standard CTAB method with a few minor modifications developed for orchid species and perennial crops (Doyle and Doyle, 1987;Sutanto et al, 2014;Elina et al, 2017;Pesik et al, 2017;Sukma et al, 2017;Sudarsono et al, 2018;Raynalta et al, 2018;Sukma et al, 2020). The SNAP primers were used for PCR with genomic DNA.…”

Section: Sequence Analysis With Snps and Evaluation Of Snap Markersmentioning

confidence: 99%

Analysis of the genetic diversity of Phalaenopsis orchids with single nucleotide polymorphisms and snap markers derived from the Pto gene

2021

SABRAOJBG

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The Pto gene is a plant gene that has been reported to be involved in resistance to bacterial pathogens. A partial genomic sequence corresponding to Pto (~449 bp) was isolated from 16 species and four hybrids of Phalaenopsis during 2017 at the Department of Agronomy and Horticulture, IPB University, Bogor, Indonesia. Multiple sequence analysis was performed to find putative single nucleotide polymorphisms (SNPs) and design the corresponding single nucleotide-amplified polymorphism (SNAP) markers, which were in turn used to estimate the genetic diversity of 25 Phalaenopsis species. In total, 20 SNPs, of which 14 were nonsynonymous, were identified from the partial Pto sequences. Eighteen SNAP primers were then developed based on these 14 nonsynonymous and four synonymous SNPs. Validation results showed that 15 SNAP primers showed a polymorphism information content exceeding 0.3, suggesting the existence of more than two alleles for this locus. Upon their use, the SNAP markers described 86% of all interspecies variability. The Pto 52, Pto 349, Pto 229, and Pto 380 SNAP markers were very informative in the determination of genetic diversity. Notably, the existence of these nonsynonymous SNPs implied the possibility of functional changes within the amino acid sequence of the putative PTO protein. Thus, the resulting differences in the activity of the PTO protein may be used to breed tolerance to pathogen infection. Further work may be required to establish a functional link between tolerance to pathogens and the presence of Pto-SNAP markers in Phalaenopsis properly.

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Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids

Cited by 2 publications

References 30 publications

Understanding of genes encoding bioactive compounds from potential medicinal plants in Indonesia as cancer cell inhibitors

Understanding of genes encoding bioactive compounds from potential medicinal plants in Indonesia as cancer cell inhibitors

Analysis of the genetic diversity of Phalaenopsis orchids with single nucleotide polymorphisms and snap markers derived from the Pto gene

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