Isolation and characterization of chitinolytic Streptomyces sp. MT7 and its antagonism towards wood-rotting fungi

Nagpure, Anand; Choudhary, Bharti; Kumar, Shanti; Gupta, Rajinder K.

doi:10.1007/s13213-013-0686-x

Cited by 27 publications

(31 citation statements)

References 31 publications

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“…Besides many factors, chitinase enzyme action on fungal pathogen cell wall seems to play an active role in antagonism (Nagpure et al . ). Out of 68 strains, only 12 strains exhibited strong chitinolytic activity as determined by degradation of colloidal chitin on CCA plates.…”

Section: Discussionmentioning

confidence: 97%

Evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants againstSclerotium rolfsiiin chickpea

Singh

Gaur

2016

J Appl Microbiol

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Section: Discussionmentioning

confidence: 97%

Evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants againstSclerotium rolfsiiin chickpea

Singh

Gaur

2016

J Appl Microbiol

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“…). As stated in many reports, extracellular production of secondary metabolites during stationary phase is related to the antibiosis toward pathogenic fungi . Broad spectrum antifungal potential of ECF of antagonist strain MT7 and its n ‐butanol extract indicates the presence of antifungal metabolite(s) in culture filtrate (Table ).…”

Section: Discussionmentioning

confidence: 97%

“…Ergosterol inhibition assay revealed the polyene type of extracellular antifungal metabolite(s) (Table ). Polyene antibiotics, e.g., nystatin or amphotericin B are known to have higher affinity toward egrosterol present in fungal cell‐wall thus causing micropores formation that disrupts the cell membrane function and hence leads to leakage of cellular constituents and fungicidal effect . The MIC values of the crude n ‐butanol extract in increasing order against C. gloeosporioides MTCC 9664, A. niger MTCC 281, G. candidum NFCCI 2848, R. stolonifer ITCC 5100, R. stolonifer NFCCI 2853, and Penicillium sp.…”

Section: Discussionmentioning

confidence: 99%

“…Antagonist S. violascens MT7 was isolated from soil of phoomdi of Loktak Lake (floating lake), Manipur, India and it was explored for its biocontrol ability to produce antifungal metabolites. It was maintained either frozen at −80 °C in 50% (w/v) glycerol or at 4 °C as 1‐week‐old slant cultures on Streptomyces agar (M1352, Hi‐media, India).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, n ‐butanol crude extract was subjected to heat (100 °C for 45 min) and proteinase K treatment (0.1 mg ml −1 for 60 min at 37 °C) to test the thermal stability and proteinaceous nature of bioactive metabolite(s), respectively, and then antifungal activity was performed with methanol as control. Ergosterol agar plate assay was also performed to determine the chemical nature of antifungal metabolite(s) present in n ‐butanol extract .…”

Section: Methodsmentioning

confidence: 99%

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Biological control of toxigenic citrus and papaya‐rotting fungi by Streptomyces violascens MT7 and its extracellular metabolites

Choudhary

Nagpure

Gupta

2015

J. Basic Microbiol.

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An Indian indigenous, Loktak Lake soil isolate Streptomyces violascens MT7 was assessed for its biocontrol potential both in vitro and in vivo against toxigenic fruit-rotting fungi. Strain MT7 exhibited broad-spectrum antifungal activity against various pathogenic postharvest fungi of citrus and papaya. In shake-flask fermentation, antagonist S. violascens MT7 highly produced extracellular antifungal metabolites in early stationary growth phase in glucose-yeast extract-malt extract (M93) broth. Both extracellular culture fluid (ECF) and its n-butanol extract showed significant broad-spectrum fungal mycelial inhibition of several tested fruit-rotting fungi. Antifungal metabolite was found to be heat stable, nonpeptidic, and polyene type antibiotic. The lowest minimum inhibitory concentration (MIC) of n-butanol extract against Colletotrichum gloeosporioides MTCC 9664 and Aspergillus niger MTCC 281 was 0.0312 and 0.0625 mg/ml, respectively. Purification of n-butanol extract through silica gel chromatography resulted in partial purification of bioactive metabolite and the TLC autobiography revealed the presence of single antifungal metabolite with Rf value of 0.755. In vivo bioassays demonstrated the biocontrol potential of tested biocontrol agents on fruit-rotting fungi. Use of cell suspension of S. violascens MT7, extracellular metabolite(s), and n-butanol extract significantly (p < 0.05) reduced sour-rot development on Citrus reticulata Blanco (oranges) and soft-rot development on papaya fruits. Therefore, these results strongly suggest a high potential for application of S. violascens MT7 and its extracellular metabolites as an effective eco-friendly alternative to synthetic fungicides for controlling toxigenic citrus and papaya-rotting fungi.

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Fungal cell-wall lytic enzymes, antifungal metabolite(s) production, and characterization fromStreptomyces exfoliatusMT9 for controlling fruit-rotting fungi

Choudhary

Nagpure

Gupta

2014

J. Basic Microbiol.

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An antifungal actinomycete strain MT9 was isolated from Loktak Lake, Manipur, India and its cultural characteristics, fatty acid methyl ester, 16S rRNA gene analysis suggests that strain MT9 is identical to Streptomyces exfoliatus. Strain MT9 displayed strong and broad-spectrum antagonism towards several fruit-rotting fungi by mycelial growth suppression. Crude fungal cell-wall lytic enzymes, i.e., chitinase, β-1,3-glucanase, and protease produced by S. exfoliatus MT9 were optimally active at pH 8.0 and 50 °C, pH 5.0 and 60 °C, pH 9.0 and 70 °C, respectively. All three mycolytic enzymes had good stability over a wide pH range of 5.0-10.0, with protease being more thermostable than both chitinase and β-1,3-glucanase. Interestingly zymogram analysis revealed that S. exfoliatus MT9 secretes six distinct chitinase isoenzymes with approximate molecular weights of 9.42, 13.93, 27.87, 36.43, 54.95, 103.27 kDa, six active protease isoenzymes with apparent molecular weights of 12.45, 30.20, 37.45, 46.32, 52.46, 131.46 kDa, and an active band of 119.39 kDa as β-1,3-glucanase enzyme. Extracellular fluid and its organic solvent extracts also exhibited inhibitory activity to various fruit-rotting fungi. The MIC value of n-butanol extract was 2-25 µg/ml against tested fruit-rotting fungi. Antifungal secondary metabolite(s) was found to be polyene in nature. To the best of our knowledge, this is the first report on extracellular production of fungal cell-wall lytic enzymes and antifungal metabolites by bioactive S. exfoliatus MT9 under submerged fermentation.

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Isolation and characterization of chitinolytic Streptomyces sp. MT7 and its antagonism towards wood-rotting fungi

Cited by 27 publications

References 31 publications

Evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants againstSclerotium rolfsiiin chickpea

Evaluation of antagonistic and plant growth promoting activities of chitinolytic endophytic actinomycetes associated with medicinal plants againstSclerotium rolfsiiin chickpea

Biological control of toxigenic citrus and papaya‐rotting fungi by Streptomyces violascens MT7 and its extracellular metabolites

Fungal cell-wall lytic enzymes, antifungal metabolite(s) production, and characterization fromStreptomyces exfoliatusMT9 for controlling fruit-rotting fungi

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