Isolation and Characterization of Chitinolytic Bacteria and Their Potential to Inhibit Plant Pathogenic Fungi

Dwi, Suryanto; Irawati, Netti; Munir, Erman

doi:10.5454/mi.5.3.8

Cited by 19 publications

(22 citation statements)

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“…Our previous study have shown that several local chitinolytic bacterial isolates inhibited the growth of pathogenic fungi Ganoderma boninense, Penicillium citrinum and F. oxysporum in vitro (Suryanto et al, 2011). In this study, we observed that all tested chitinolytic bacterial isolates effectively suppressed the growth, and decreased anthracnose severity and intensity caused by C. gloeosporioides.…”

Section: Discussionsupporting

confidence: 57%

“…These isolates were not morphologically and biochemically characterized except that of KR05 and LK08 (Suryanto et al, 2011). The isolates were kept at 30°C in a modified salt medium (0.7 g K 2 HPO 4 , 0.3 g KH 2 PO 4 , 0.5 g MgSO 4 .7H 2 O, 0.01 g FeSO 4 .7H 2 O, 0.001 g ZnSO 4 , and 0.001 g MnCl 2 in 1.000 ml) containing 2% (w/v) chitin colloidal (MSMC) agar, with a pH of 6.8.…”

Section: Chitinolytic Bacterial Isolatesmentioning

confidence: 99%

“…The hyphae were examined under light microscope and compared with normal ones. (Suryanto et al, 2011).…”

Section: Observation Of Hyphal Abnormalitymentioning

confidence: 99%

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Utilization of chitinolytic bacterial isolates to control anthracnose of cocoa leaf caused by Colletotrichum gloeosporioides

Suryanto¹,

Wahyuni²,

Siregar³

et al. 2014

Afr. J. Biotechnol.

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Colletotrichum spp. are causal agents of anthracnose in many plant species. Biological control of Colletotrichum spp. utilizing bacterial isolates and fungi has been reported. However, chitinolytic bacterial isolate utilization to control anthracnose of cocoa leaf has not seemingly been studied yet. In this study, we used chitinolytic bacterial isolates to reduce anthracnose severity and incidence caused by Colletotrichum gloeosporioides. Identification of the chitinolytic isolates was conducted for their morphological and biochemical traits, and the sequencing of 16S rRNA was to know their related species. Assay of antagonistic bacterial chitinolytic to C. gloeosporioides was conducted in minimum salt medium agar with 2% colloidal chitin as sole carbon source. To examine ability of the chitinolytic isolates in reducing anthracnose severity and incidence, cocoa leaves were treated with the isolates prior infestation of the conidia. Identification of 16S rRNA showed that KR05, LK08, BK13, BK15 and BK17 isolates were Enterobacter sp., Enterobacter cloacae, Bacillus sp., Enterobacter sp., and Bacillus sp., respectively. All chitinolytic isolates inhibited growth of C. gloeosporioides in vitro to some extent. Microscopic studies showed morphological abnormalities of C. gloeosporioides hyphae that is, broken, lytic, rolled, twisted, curled and abnormal branching of hyphae as a result of antagonistic mechanism caused by the chitinolytic isolates. All chitinolytic isolates were able to reduce anthracnose severity and incidence on cocoa leaves from 0.8 to 3.2% and 4 to 12%, respectively.

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Section: Discussionsupporting

confidence: 57%

Section: Chitinolytic Bacterial Isolatesmentioning

confidence: 99%

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Utilization of chitinolytic bacterial isolates to control anthracnose of cocoa leaf caused by Colletotrichum gloeosporioides

Suryanto¹,

Wahyuni²,

Siregar³

et al. 2014

Afr. J. Biotechnol.

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“…(Kim et al, 2008). Suryanto et al (2011) showed that chitinolytic bacterial isolates inhibited the growth of Fusarium oxysporum, G. boninense, and Penicillium semitectum. Furthermore, chitinolytic bacteria reduced the incidence of red pepper seedling wilt caused by F. oxysporum (Suryanto et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

A possibility of chitinolytic bacteria utilization to control basal stems disease caused by Ganoderma boninense in oil palm seedling

Suryanto¹

2012

Afr. J. Microbiol. Res.

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The utilization of chitinolytic bacterial isolates Enterobacter sp. KR05, Enterobacter cloacae LK08, Bacillus sp. BK13, Enterobacter sp. BK15, and Bacillus sp. BK17 to control basal stem rot disease caused by Ganoderma boninense in oil palm seedling was studied. Antagonistic assay of chitinolytic bacterial isolates to G. boninense was conducted in minimum salt medium agar with 2% colloidal chitin as sole carbon source. To examine ability of the isolates in reducing basal stem rot disease incidence, the 3 to 4 months old of oil palm seedlings were treated by pouring oil palm seedling growing media with chitinolytic bacterial isolates a day prior infestation of G. boninense spores. The result showed that all chitinolytic isolates inhibited the growth of G. boninense in vitro. Hyphal abnormalities that is, dwarf, tiny, curled, twisted and bulby hyphae were observed after antagonistic assay. All chitinolytic isolates were able to reduce the disease incidence on the oil palm seedling to some extent. The isolates might infest into the oil palm seedling root as endophytes.

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“…raphani, Phytophthora cinnamomi, P. nicotianae and Rhizoctonia solani (Kavraulakis et al, 2010). Our previous studies also showed that chitinolytic bacterial isolates reduce disease incidence of G. boninense in oil palm seedling (Suryanto et al, 2012) and inhibited some plant pathogenic fungal growth (Suryanto et al, 2011).…”

Section: Discussionmentioning

confidence: 94%

EXAMINATION OF CHITINOLYTIC BACTERIA IN ALGINATE-CHITOSAN ENCAPSULATION ON CHILI SEED AGAINST DAMPING OFF CAUSED BY <i>FUSARIUM OXYSPORUM</i>

Suryanto¹,

Indarwan²,

Munir³

2012

American Journal of Agricultural and Biological Sciences

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One alternative in biological control of plant pathogenic fungi is to provide biological agent inoculant in coated seed. This study was to evaluate potential control and viability of two chitinolytic bacteria, Enterobacter sp. BK15 and Bacillus sp. BK17 of alginate-chitosan encapsulation coated on chili seed against damping off caused by Fusarium oxysporum. Chili seed beads were stored at refrigerator and room temperature. Examination of the bacteria ability in reducing seedling off was conducted by growing and treating the seed beads with F. oxysporum inoculum. Bacterial cell viability was measured by growing grinded chili bead in minimum salt medium with chitin as sole C source. Re-isolation of infected seedling was done by growing it in potato dextrose agar. The result showed that the bacteria in alginate-chitosan coated seeds reduced chili damping off. Chili seedling height and dry-weight did not differ with those of (-) control, treatment with no bacterial and fungal inoculation and differed with (+) control, treatment of fungal inoculation only. Re-isolation of infected seedling showed similar fungal characterization of inoculated F. oxysporum. Bacterial cell viability decreased significantly in 30 days of storage both at refrigerator and room temperature. However, cell viability stored at refrigerator was higher rather than that of room temperature.

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Isolation and Characterization of Chitinolytic Bacteria and Their Potential to Inhibit Plant Pathogenic Fungi

Cited by 19 publications

References 0 publications

Utilization of chitinolytic bacterial isolates to control anthracnose of cocoa leaf caused by Colletotrichum gloeosporioides

Utilization of chitinolytic bacterial isolates to control anthracnose of cocoa leaf caused by Colletotrichum gloeosporioides

A possibility of chitinolytic bacteria utilization to control basal stems disease caused by Ganoderma boninense in oil palm seedling

EXAMINATION OF CHITINOLYTIC BACTERIA IN ALGINATE-CHITOSAN ENCAPSULATION ON CHILI SEED AGAINST DAMPING OFF CAUSED BY <i>FUSARIUM OXYSPORUM</i>

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