Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties

El-Beltagy, A.S.; Nishioka, K; Suzuki, Hisa; Satô, Tadashi; Sato, Yo Ichiro; Morisaki, Hisao; Mitsui, Hisayuki; Minamisawa, Kiwamu

doi:10.1080/00380768.2000.10409127

Cited by 203 publications

(114 citation statements)

References 39 publications

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“…The genus Microbacterium was previously isolated from other plants such as maize (Rijavec et al, 2007) and rice (Elbeltagy et al, 2000). The Microbacterium isolates from T. elegans were grouped with more similarity in the clade isolates of Actinomycetales.…”

Section: Molecular Identification Of Isolates Based On Partial 16s Rdmentioning

confidence: 98%

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Rhoden

García

Silva³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Various organisms such as fungi and bacteria can live inside plants, inhabiting the aerial parts (primarily the leaves) without causing damage. These microorganisms, called endophytes, produce an extensive variety of compounds that can be useful for medical and agronomic purposes. Trichilia elegans A. Juss., belonging to the Meliaceae family, shows wide dispersion in South America, and phytochemical analyses from these plants and endophyte isolates have shown biological activity. Accordingly, the aim of this study was to verify the diversity of bacterial endophytes from T. elegans using partial sequencing of 16S rRNA, followed by phylogenetic analysis. Isolation was performed by cutting the leaves, after disinfection with 5% sodium hypochlorite (NaOCl), in 1-2-mm 2 fragments, which were equally placed on dishes containing TSA and fungicide BENLATE at 75 μg/mL. All dishes were incubated at 28°C in the biochemical oxygen demand system for 5 days and periodically checked. Afterwards, the colonization frequency (%) was determined: (number of fragments colonized by bacteria/total number of fragments) x 100. Three isolations between September 2011 and March 2012 were performed; the growth frequency ranged between 1.6 and 13.6%. Following sequencing of 16S rRNA and phylogenetic analysis, the genera identified were: Staphylococcus, Bacillus, Microbacterium, Pseudomonas, and Pantoea. These results will provide important knowledge on the diversity of endophytic bacteria inhabiting medicinal plants, and a better understanding of the microbiome of T. elegans would reinforce the necessity of endophyte studies with a focus on their future applications in biotechnological areas of agriculture, medicine, and the environment.

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Section: Molecular Identification Of Isolates Based On Partial 16s Rdmentioning

confidence: 98%

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Rhoden

García

Silva³

et al. 2015

Genet. Mol. Res.

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“…Gram staining, motility, and production of pectinase and cellulase were assayed as described previously (9). Three isolates that were close to Herbaspirillum sp.…”

Section: Methodsmentioning

confidence: 99%

Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species

El-Beltagy

Nishioka

Satô

et al. 2001

Appl Environ Microbiol

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429

302

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Nitrogen-fixing bacteria were isolated from the stems of wild and cultivated rice on a modified Rennie medium. Based on 16S ribosomal DNA (rDNA) sequences, the diazotrophic isolates were phylogenetically close to four genera: Herbaspirillum, Ideonella, Enterobacter, and Azospirillum. Phenotypic properties and signature sequences of 16S rDNA indicated that three isolates (B65, B501, and B512) belong to the Herbaspirillum genus. To examine whether Herbaspirillum sp. strain B501 isolated from wild rice, Oryza officinalis, endophytically colonizes rice plants, the gfp gene encoding green fluorescent protein (GFP) was introduced into the bacteria. Observations by fluorescence stereomicroscopy showed that the GFP-tagged bacteria colonized shoots and seeds of aseptically grown seedlings of the original wild rice after inoculation of the seeds. Conversely, for cultivated rice Oryza sativa, no GFP fluorescence was observed for shoots and only weak signals were observed for seeds. Observations by fluorescence and electron microscopy revealed that Herbaspirillum sp. strain B501 colonized mainly intercellular spaces in the leaves of wild rice. Colony counts of surface-sterilized rice seedlings inoculated with the GFP-tagged bacteria indicated significantly more bacterial populations inside the original wild rice than in cultivated rice varieties. Moreover, after bacterial inoculation, in planta nitrogen fixation in young seedlings of wild rice, O. officinalis, was detected by the acetylene reduction and 15 N 2 gas incorporation assays. Therefore, we conclude that Herbaspirillum sp. strain B501 is a diazotrophic endophyte compatible with wild rice, particularly O. officinalis.

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“…So far, a number of bacterial isolates from the rice phyllosphere have been characterized (Elbeltagy et al, 2000;Madhaiyan et al, 2007Madhaiyan et al, , 2009Mano et al, 2007) and potential beneficial interactions of phyllosphere bacteria with rice plants, such as plant growth promotion, for example, by bacterial nitrogen fixation or plant hormone production, and protection against pathogens have been studied (Madhaiyan et al, 2004;Maliti et al, 2005;De Costa et al, 2008;Yang et al, 2008;Chinnadurai et al, 2009;Pedraza et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Metaproteogenomic analysis of microbial communities in the phyllosphere and rhizosphere of rice

et al. 2011

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The above-and below-ground parts of rice plants create specific habitats for various microorganisms. In this study, we characterized the phyllosphere and rhizosphere microbiota of rice cultivars using a metaproteogenomic approach to get insight into the physiology of the bacteria and archaea that live in association with rice. The metaproteomic datasets gave rise to a total of about 4600 identified proteins and indicated the presence of one-carbon conversion processes in the rhizosphere as well as in the phyllosphere. Proteins involved in methanogenesis and methanotrophy were found in the rhizosphere, whereas methanol-based methylotrophy linked to the genus Methylobacterium dominated within the protein repertoire of the phyllosphere microbiota. Further, physiological traits of differential importance in phyllosphere versus rhizosphere bacteria included transport processes and stress responses, which were more conspicuous in the phyllosphere samples. In contrast, dinitrogenase reductase was exclusively identified in the rhizosphere, despite the presence of nifH genes also in diverse phyllosphere bacteria.

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Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties

Cited by 203 publications

References 39 publications

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species

Metaproteogenomic analysis of microbial communities in the phyllosphere and rhizosphere of rice

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