Isolation and characterization of exopolysaccharide with immunomodulatory activity from fermentation broth of Morchella conica

Abstract

Background and the purpose of this studyMushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. In vitro and in vivo studies suggest that certain polysaccharides affect immune system function. Morchella conica (M. conica) is a species of rare edible mushroom whose multiple medicinal functions have been proven. Thus, the objective of this study is to isolate and characterize of exopolysaccharide from submerged mycelial culture of M. conica, and to evaluate its immunomodulatory activity.MethodsA water-soluble Morchella conica Polysaccharides (MCP) were extracted and isolated from the fermentation broth of M. conica through a combination of DEAE-cellulose and Sephacryl S-300 HR chromatograph. NMR and IR spectroscopy has played a developing role in identification of polysaccharide with different structure and composition from fungal and plant sources, as well as complex glycosaminoglycans of animal origin. Thus, NMR and IR spectroscopy were used to analyze the chemical structure and composition of the isolated polysaccharide. Moreover, the polysaccharide was tested for its immunomodulatory activity at different concentrations using in vitro model.ResultsThe results showed that MCP may significantly modulate nitric oxide production in macrophages, and promote splenocytes proliferation. Analysis from HPLC, infrared spectra and nuclear magnetic resonance spectroscopy showed that MCP was a homogeneous mannan with an average molecular weight of approximately 81.2 kDa. The glycosidic bond links is →6)-α-D-Man p-(1→.ConclusionThe results suggested that the extracted MCP may modulate nitric oxide production in macrophages and promote splenocytes proliferation, and it may act as a potent immunomodulatory agent.

Purification, characterization, antioxidant activity and anti-aging of exopolysaccharides by Flammulina velutipes SF-06

“…4d, the monosaccharides contents of EPS-2 were Rha (94.39 %) and Gal (5.61 %) with the molar ratio of 44.5:2.9. Some reports showed that the monosaccharide composition of EPS of M. conica (Su et al 2013), Pycnoporus sanguineus (Cao et al 2014) and B. aereus was Man, Glu, Glu, respectively, which were differ from our results of the EPS by F. velutipes SF-06. According to the above results, the monosaccharide composition of EPS was also different from that of EPS-1 or EPS-2.…”

“…3 FT-IR spectra of EPS-1 and EPS-2 at the range of 400-4,000 cm -1 C=O asymmetric stretching vibration absorption peak and the peaks at 1,618.19 and 1,457.17 cm -1 indicated the presence of -NH 2 and -COOH groups in EPS-2 (Cerna et al 2003). According to the results stated above, the EPS by F. velutipes SF-06 is novel in its structure, compared to the reports on Morchella conica of EPS (Su et al 2013) and Boletus aereus ).…”

Natural Bioactive Compounds from Fungi as Potential Candidates for Protease Inhibitors and Immunomodulators to Apply for Coronaviruses

“…Activating macrophages and cytokines [147,148] Grifolan Grifola frondosa Activating macrophages and lymphokines [149] Lentinan Lentinus edodes T-cell-oriented adjuvant [149] PS-G Ganoderma lucidum Activating macrophages and T lymphocytes [135,136] Schizophyllan Schizophyllum commune Activating T cell, increasing interleukin and TNF-α production [150] Terpenoids…”

Biotechnological challenges and perspectives of using exopolysaccharides

“…An appropriate protocol for the isolation, quantification and characterization of EPS is essential so that each step of the process, fermentation medium, isolation purpose and characterization method is carefully adjusted for each type of previously planned EPS. 14,15…”