Isolation and characterization of "Flexispira rappini" from laboratory mice

Schauer, David B.; Ghori, Nafisa; Falkow, Stanley

doi:10.1128/jcm.31.10.2709-2714.1993

Cited by 76 publications

(41 citation statements)

References 19 publications

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“…To our knowledge, organisms of the Helicobacter ("Flexispira") taxa (including H. bilis) have not been isolated from cats. However, such organisms have been cultured from the feces of three dogs and their owners, diarrheic children, and rodents (22,33,46,49). They are also increasingly isolated from the blood of immunocompromised patients, including two that had a history of contact with puppies (51,56).…”

Section: Discussionmentioning

confidence: 99%

Coinfection of Enteric Helicobacter spp. and Campylobacter spp. in Cats

et al. 2001

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During a 6-year period, 64 of 227 commercially reared cats had microaerobic bacteria isolated from their feces. All the isolates were initially identified as Campylobacter-like organisms based on biochemical and phenotypic characteristics. DNA extractions from 51 of these isolates were subjected to PCR using primers specific for Helicobacter spp. and Campylobacter spp. Of the isolates, 92% (47 of 51 isolates) were positive for Campylobacter spp., 41% (21 of 51 isolates) were positive for Helicobacter spp., 33% (17 of 51 isolates) were positive for both genera, 59% (30 of 51 isolates) were positive only for Campylobacter spp., and 8% (4 of 51) were positive only for Helicobacter spp. Sixteen of the 47 Campylobacter-positive cultures were positive for more than one Campylobacter spp. Based on a species-specific PCR assay, 83% of the isolates were identified as Campylobacter helveticus, 47% of the isolates were identified as Campylobacter upsaliensis, and 6% of the isolates were classified as Campylobacter jejuni. The 1.2-kb PCR products of the 16S rRNA genes of 19 Helicobacter species isolates were subjected to restriction fragment length polymorphism (RFLP) analysis. Of the five different RFLP patterns obtained, two clustered with Helicobacter ("Flexispira") taxon 8, one clustered with Helicobacter bilis, one clustered with Helicobacter canis, and the remaining pattern was closely related to a novel Helicobacter sp. strain isolated from a woodchuck. The sequence data for the 16S rRNA genes of 10 Helicobacter spp. validated the RFLP-based identification of these isolates. This study demonstrated that biochemical and phenotypic characteristics of microaerobic organisms in cat feces were insufficient to characterize mixed Helicobacter and Campylobacter infections. Molecular structure-based diagnostics using genus-and speciesspecific PCR, RFLP analysis, and 16S rRNA sequence analysis enabled the identification of multiple microaerobic species in individual animals. The clinical relevance of enteric Helicobacter and Campylobacter coinfection in cats will require further studies.Cats are recognized reservoirs for enteric Campylobacter spp., including Campylobacter jejuni, Campylobacter coli, Campylobacter upsaliensis, and Campylobacter helveticus (5,13,20,37,58). C. jejuni and C. coli are among the most frequently encountered human enteric pathogens worldwide (2, 3). C. upsaliensis, a catalase-negative or weakly positive Campylobacter sp. initially isolated from diarrheic or nondiarrheic domestic dogs and cats (20,48), has also been associated with enteritis (25, 44, 55) and bacteremia (31, 39, 44) in humans. More-serious illnesses, including spontaneous abortion and hemolytic-uremic syndrome, have also been reported for a human infected with C. upsaliensis (27). Campylobacter infections, particularly C. jejuni infections, are zoonotic and are a particular problem among puppies and kittens from shelters (13, 18, 45). C. helveticus, which is closely related to C. upsaliensis, has also been isolated from domestic cats an...

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Section: Discussionmentioning

confidence: 99%

Coinfection of Enteric Helicobacter spp. and Campylobacter spp. in Cats

et al. 2001

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“…The organisms bear more of a resemblance to`F. rappini', H. bilis and H. canis, some closely related bacilli isolated from the intestine of various species (Lockard and Boler, 1970;Schauer et al, 1993;Stanley et al, 1993;Fox et al, 1995;Foley et al, 1999). The presence of a single periplasmic ®lament together with the lack of a basal plate at the point of¯agella insertion strongly support that the organism is actually`F.…”

Section: Discussionmentioning

confidence: 82%

“…Helicobacter species like`F. rappini' and H. bilis are regarded as normal colonizers of the lower gastrointestinal tract in the mouse (Schauer et al, 1993;Fox et al, 1995). The same is true for H. cinaedi in hamsters (Gebhart et al, 1989) and H. canis in the dog (Stanley et al, 1993).…”

Section: Discussionmentioning

confidence: 95%

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Fatal Gastrointestinal Infection with ‘Flexispira rappini’‐Like Organisms in a Cat

Kipar

Weber

Menger

et al. 2001

Journal of Veterinary Medicine, Series B

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A 4-month-old male British Blue cat with catarrhal to haemorrhagic enteritis showed massive colonization of the stomach, small intestine and caecum with spiral-shaped bacilli. In the stomach, organisms were located in foveolae and gland lumina and within unaltered and degenerate epithelial cells. Inflammatory infiltration was moderate and T cell dominated. In the intestine, bacilli were found in the gut lumen, berween villi, in crypt lumina and within epithelial cells. Degeneration of crypt epithelial cells as well as crypt dilation and moderate to massive macrophage-dominated infiltration of the mucosa and submucosa were observed. Immunohistochemically, bacilli were positive with an antibody against Helicobacter. Ultrastructurally, the organisms strongly resembled 'Flexispira rappini', a spiral-shaped Helicobacter species known as a normal intestinal colonizer in dogs and mice.

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“…Both species were isolated from the livers of animal with hepatitis [8,9]. H. rappini is associated with abortion in sheep and intestinal disease in animals and humans [10], and was also isolated from the colons and ceca of mice [11]. Recently, urease-negative H. typhlonicus was isolated from interleukin-10-de¢cient mice with colitis and typhlitis, and also from immunode¢cient CB-17 scid/scid mice [12,13].…”

Section: Introductionmentioning

confidence: 99%

Helicobacter muricola sp. nov., a novel Helicobacter species isolated from the ceca and feces of Korean wild mouse (Mus musculus molossinus)

Won¹

2002

FEMS Microbiology Letters

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A slowly growing microaerophilic Helicobacter strain was isolated from the ceca and fecal pellets of Korean wild mice (Mus musculus molossinus). This bacterial strain possessed a pair of nonsheathed bipolar flagella, was positive for urease, catalase and oxidase, and reduced nitrate to nitrite. It proved susceptible to nalidixic acid and resistant to cephalodine, and did not hydrolyze hippurate. On the basis of phenotypic characteristics and 16S rRNA gene sequence analysis, the isolate represents a new species of the genus Helicobacter, for which the name Helicobacter muricola sp. nov. is proposed; the type strain of the new species is w‐06T (GenBank accession number AF264783).

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Isolation and characterization of "Flexispira rappini" from laboratory mice

Cited by 76 publications

References 19 publications

Coinfection of Enteric Helicobacter spp. and Campylobacter spp. in Cats

Coinfection of Enteric Helicobacter spp. and Campylobacter spp. in Cats

Fatal Gastrointestinal Infection with ‘Flexispira rappini’‐Like Organisms in a Cat

Helicobacter muricola sp. nov., a novel Helicobacter species isolated from the ceca and feces of Korean wild mouse (Mus musculus molossinus)

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