Isolation and characterization of heterotepalins, type 1 ribosome-inactivating proteins from Phytolacca heterotepala leaves

Maro, Antimo Di; Daniele, Addolorata; Casoria, Paolo; Parente, Augusto

doi:10.1016/j.phytochem.2006.12.002

Cited by 41 publications

(18 citation statements)

References 29 publications

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“…During the purification of type 1 ribosome-inactivating proteins from P. dioica leaves (19)(20)(21), we obtained different chromatographic profiles between summer (June) and autumn http://bmbreports.org BMB reports Fig. 1.…”

Section: Purification Of P Dioica Peroxidasementioning

confidence: 99%

“…Materials for chromatography were described elsewhere (19). ABTS [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid], guaiacol, pcoumaric acid and indole-3-acetic acid were purchased from Sigma-Aldrich (Milan, Italy).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…The purification procedure has been previously reported (19,21). Leaves (100 g) were homogenized in 500 ml of buffer A by 20 s bursts at full power using a Waring blender (Waring Products; Torrington, USA).…”

Section: Protein Purificationmentioning

confidence: 99%

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Purification and enzymatic properties of a peroxidase from leaves of Phytolacca dioica L. (Ombú tree)

Guida¹,

Criscuolo²,

Tamburino³

et al. 2011

BMB Reports

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Section: Purification Of P Dioica Peroxidasementioning

confidence: 99%

Section: Chemicals and Reagentsmentioning

confidence: 99%

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Purification and enzymatic properties of a peroxidase from leaves of Phytolacca dioica L. (Ombú tree)

Guida¹,

Criscuolo²,

Tamburino³

et al. 2011

BMB Reports

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“…In particular, a rich group of angiosperms expressing many type-1 RIPs is the Phytolaccaceae family [24], where the prototype of type-1 RIPs, pokeweed antiviral protein (PAP) from leaves of Phytolacca americana L., was isolated given its pronounced antiviral activity [25]. Since then, many others type-1 RIPs were isolated from Phytolaccaceae species such as Phytolacca dodecandra L'Herit [26], Phytolacca heterotepala H. Walter [27,28], Phytolacca insularis [29] Nakai, and Phytolacca dioica L. [30]. In particular, from seeds and from adult and young leaves of P. dioica plant, three (PD-S1-3; [31,32]), four (PD-L1-4; [33,34]), and two (dioicin 1 and 2; [35][36][37]) type-1 RIPs, respectively, were isolated and extensively characterized.…”

Section: Introductionmentioning

confidence: 99%

Antiviral Activity of PD-L1 and PD-L4, Type 1 Ribosome Inactivating Proteins from Leaves of Phytolacca dioica L. in the Pathosystem Phaseolus vulgaris–Tobacco Necrosis Virus (TNV)

Bulgari

Ragucci

Faoro

et al. 2020

Toxins

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Using the pathosystem Phaseolus vulgaris–tobacco necrosis virus (TNV), we demonstrated that PD-L1 and PD-L4, type-1 ribosome inactivating proteins (RIPs) from leaves of Phytolacca dioica L., possess a strong antiviral activity. This activity was exerted both when the RIPs and the virus were inoculated together in the same leaf and when they were inoculated or applied separately in the adaxial and abaxial leaf surfaces. This suggests that virus inhibition would mainly occur inside plant cells at the onset of infection. Histochemical studies showed that both PD-L1 and PD-L4 were not able to induce oxidative burst and cell death in treated leaves, which were instead elicited by inoculation of the virus alone. Furthermore, when RIPs and TNV were inoculated together, no sign of H2O2 deposits and cell death were detectable, indicating that the virus could have been inactivated in a very early stage of infection, before the elicitation of a hypersensitivity reaction. In conclusion, the strong antiviral activity is likely exerted inside host cells as soon the virus disassembles to start translation of the viral genome. This activity is likely directed towards both viral and ribosomal RNA, explaining the almost complete abolition of infection when virus and RIP enter together into the cells.

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“…The highest number of RIPs was found in Caryophyllaceae, Sambucaceae, Cucurbitaceae, Euphorbiaceae, and Poaceae [4,11]. In addition, several isoforms of type 1 RIPs were purified from Phytolaccaceae [4,12,13] and in particular from Phytolacca dioica L., a woody plant introduced in Italy from South America.…”

Section: Introductionmentioning

confidence: 99%

Structural characterization of dioicin 1 from Phytolacca dioica L. gains novel insights into phylogenetic relationships of Phytolaccaceae type 1 RIPs

Russo

Severino

Parente

et al. 2015

Biochemical and Biophysical Research Communications

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Isolation and characterization of heterotepalins, type 1 ribosome-inactivating proteins from Phytolacca heterotepala leaves

Cited by 41 publications

References 29 publications

Purification and enzymatic properties of a peroxidase from leaves of Phytolacca dioica L. (Ombú tree)

Purification and enzymatic properties of a peroxidase from leaves of Phytolacca dioica L. (Ombú tree)

Antiviral Activity of PD-L1 and PD-L4, Type 1 Ribosome Inactivating Proteins from Leaves of Phytolacca dioica L. in the Pathosystem Phaseolus vulgaris–Tobacco Necrosis Virus (TNV)

Structural characterization of dioicin 1 from Phytolacca dioica L. gains novel insights into phylogenetic relationships of Phytolaccaceae type 1 RIPs

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