Isolation and Characterization of Hydrocarbon-Degrading Bacteria in Top and Subsoil of selected Mechanic Workshops in Benin City Metropolis, Nigeria

Ebakota, Omonigho Daniel; Osarueme, Johnson Osazee; Gift, Onyinye Nwaeze; Odoligie, Imarhiagbe; Osazee, Joseph Osamudiamen

doi:10.4314/jasem.v21i4.3

Cited by 9 publications

(11 citation statements)

References 12 publications

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“…Landfarming soil contaminated with petroleum waste has been shown to be a reservoir of microbial communities holding genes involved in the degradation of oil compounds [ 23 ]. Additionally, previous studies have reported the isolation of bacterial strains from diverse genera with the ability to degrade petroleum hydrocarbons [ 10 , 24 , 25 , 26 ]. Particularly, the investigation of the microbial communities in SGP and their functional profiles will be communicated in another study.…”

Section: Discussionmentioning

confidence: 99%

Great Abilities of Shinella zoogloeoides Strain from a Landfarming Soil for Crude Oil Degradation and a Synergy Model for Alginate-Bead-Entrapped Consortium Efficiency

et al. 2022

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Oil contamination is of great concern worldwide and needs to be properly addressed. The present work aimed to contribute to the development of bacterial consortia for oil recovery. We investigated the community structure of a landfarming-treated soil (LF2) by metagenomics to unravel the presence of hydrocarbon degraders. Moreover, we isolated Shinella zoogloeoides LFG9 and Bacillus swezeyi LFS15 from LF2 and combined them with Pseudomonas guguanensis SGPP2 isolated from an auto mechanic workshop soil to form the mixed consortium COG1. Bacterial isolates were tested for biosurfactant production. Additionally, the bioremediation potential of COG1 was studied as free and entrapped consortia by gas chromatography-mass spectrometry, in comparison to the single strains. Results revealed the presence of Actinobacteria (66.11%), Proteobacteria (32.21%), Gammaproteobacteria (5.39%), Actinomycetales (65.15%), Burkholderiales (13.92%), and Mycobacterium (32.22%) taxa, indicating the presence of hydrocarbon degraders in soil LF2. All three isolated strains were biosurfactant producers capable of degrading crude oil components within 14 days. However, Shinella zoogloeoides LFG9 performed best and was retained as candidate for further bioremediation investigation. In addition, COG1 performed better when immobilized, with entrapment effectiveness manifested by increased fatty acids and aromatic compound degradation. Attempt to improve crude oil biodegradation by adding surfactants failed as sodium dodecyl sulfate restrained the immobilized consortium performance.

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Section: Discussionmentioning

confidence: 99%

Great Abilities of Shinella zoogloeoides Strain from a Landfarming Soil for Crude Oil Degradation and a Synergy Model for Alginate-Bead-Entrapped Consortium Efficiency

et al. 2022

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“…A variety of technologies are currently available to treat soil contaminated with hazardous materials, including excavation and containment in secured landfills, vapour extraction, stabilization and solidification, soil flushing, soil washing, solvent extraction, etc. [9]. Many of these technologies, however, are either costly or do not result in complete destruction of pollution.…”

Section: Introductionmentioning

confidence: 99%

“…There is, therefore, an increasing interest in cheap and eco-friendly remediation processes via the use of bioremediation [4]. Bioremediation is a natural process that relies on bacteria, fungi and plants to degrade pollutants as they carry out their normal life functions [9]. Bioremediation entails the application of biological processes involving enzymatic activities of microorganisms to degrade, transform and or essentially remove pollutants resulting from the accidental discharge of petroleum products and industrial processes in our environment [9].…”

Section: Introductionmentioning

confidence: 99%

“…Many microorganisms have the ability to utilize hydrocarbons as the sole source of carbon, as energy for metabolic activities and these microorganisms are widely distributed in nature [9]. The ability to isolate certain hydrocarbon-degrading microorganisms from the oil-polluted environments is evidence that these microorganisms are the active degraders of hydrocarbon pollutants in the environment [1].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of indigenous hydrocarbon Degrading-microbial niche for effective bioremediation of petroleum contaminated soil

Makut¹,

Oyegue²,

Owuna³

et al. 2022

BIOMED nat. appl. sci.

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Mishandling of petroleum products has been a significant source of environmental pollution and health hazards. Many microorganisms have the ability to utilize hydrocarbons as the sole source of carbon and these microorganisms are widely distributed in nature. Five (5) sites in Keffi, Nigeria were sampled to isolate hydrocarbon utilizing bacteria and fungi. The hydrocarbon products utilized were petrol, gear oil and engine oil. The hydrocarbon utilization was determined using the spectrophotometric method. A total of two (2) bacteria and two (2) fungi species were identified as the highest utilizers of the hydrocarbon products. The hydrocarbon utilization rate at the best temperature (37OC), pH (7) and time (28 days) was assessed. The utilization (mg/ml) of Pseudomonas aeruginosa for petrol ranges from 1.97±0.05, 1.31±0.034 for gear oil and 1.52±0.035 for engine oil. Alcaligenes faecalis utilization ranges from 2.2±0.022 for petrol, 1.57±0.031, for gear oil and 1.86± 0.034 for engine oil. Trichoderma harzianum ranges from 1.98 ±0.012 for petrol, 1.23±0.003 for gear oil and 1.73±0.008 for engine oil. Purpureocillium lilacinum ranges from 1.98±0.03 for petrol, 0.92±0.006 for gear oil and 1.39±0.035 for engine oil. The effect of microbial consortium on hydrocarbon utilization (mg/ml) for Pseudomonas aeruginosa and Alcaligenes faecalis ranges from 1.83 ±0.035 for petrol, 1.33 ± 0.023 for gear oil and 1.46 ± 0.015 for engine oil. Trichoderma harzianum and Purpureocillium lilacinum range from 1.88 ± 0.041 for petrol, 1.45 ± 0.026 for gear oil and 1.63 ± 0.011 for engine oil. While a microbial consortium of Pseudomonas aeruginosa, Alcaligenes faecalis, Trichoderma harzianum and Purpureocillium lilacinum ranges from 2.09 ± 0.002 for Petrol, 1.85 ± 0.031 for gear oil and 1.97 ± 0.034 for engine oil. P.aeruginosa, A. faecalis, T. harzianum and P. lilacinum constitute an effective microbial consortium for the bioremediation of hydrocarbon polluted soil.

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“…Other studies in Nigeria have also isolated Pseudomonas fluorescens, Pseudomonas aeruginosa, Bacillus subtilis and others from crude oil polluted streams in Nigeria (Adebusoye et al, 2006). Studies on the isolation and characterization of hydrocarbondegrading bacteria from the top soil of contaminated mechanic workshops have also been carried out in Nigeria (Ebakota et al, 2017). This study was therefore initiated to isolate and characterize hydrocarbon degrading bacteria from top soil of two dump sites for old farm tractors in Oxford, Pennsylvania.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Petroleum Hydrocarbon Degrading Bacteria from Polluted Top Soil Samples in Oxford, Pennsylvania

Wonyengibarakemi¹,

Chikwem²,

Chikwem³

2019

AIJB

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Petroleum hydrocarbon degrading bacteria were isolated from two polluted sites in Oxford, Pennsylvania by enrichment techniques using Bushnell Hass medium. No isolate in this study utilized gasoline; however, two isolates utilized engine oil and three, utilized kerosene. Identification by Gram stain, morphological, biochemical, deoxyribonucleic acid and fatty acid methyl ester tests showed that three isolates were Pseudomonas plecoglossicida, one was Pseudomonas nitroreducens and the last was Achromobacterdenitrificans. This study corroborates that of other researchers that hydrocarbon degrading bacteria can be found in all types of contaminated and polluted environments; they can therefore be enriched and utilized for the remediation of oil polluted environments. We recommend that a culture bank for the collection and preservation of microorganisms specific for bioremediation be established to enable speedy access whenever cases of environmental pollution by oil products are reported.

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Isolation and Characterization of Hydrocarbon-Degrading Bacteria in Top and Subsoil of selected Mechanic Workshops in Benin City Metropolis, Nigeria

Cited by 9 publications

References 12 publications

Great Abilities of Shinella zoogloeoides Strain from a Landfarming Soil for Crude Oil Degradation and a Synergy Model for Alginate-Bead-Entrapped Consortium Efficiency

Great Abilities of Shinella zoogloeoides Strain from a Landfarming Soil for Crude Oil Degradation and a Synergy Model for Alginate-Bead-Entrapped Consortium Efficiency

Optimization of indigenous hydrocarbon Degrading-microbial niche for effective bioremediation of petroleum contaminated soil

Isolation and Identification of Petroleum Hydrocarbon Degrading Bacteria from Polluted Top Soil Samples in Oxford, Pennsylvania

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