Isolation and Characterization of <i>Mycoplasma mycoides</i> Subspecies <i>capri</i> from Milk of Natural Goat Mastitis Cases

Kumar, Vijay; Rana, Rajneesh; Mehra, Somya; Rout, Pramod Kumar

doi:10.1155/2013/593029

Cited by 18 publications

(24 citation statements)

References 15 publications

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“…This indicates and confirms the presence of Mycoplasma infection in Chongqing area. Moreover, the isolation rate (5.9%) was found to be in agreement with Kumar et al (14,15); however, our isolation rate seems to be quite low in contrast to the 25% -71% obtained by Gil et al (10).…”

Section: Discussionsupporting

confidence: 92%

“…Mmc and Mcc are reported to cause a pattern of disease called contagious agalactia syndrome (CA) including mastitis, arthritis, pulmonary diseases, and septicaemia especially in goats (25,26,29). All three caprine pathogens can be identified and distinguished by both PCR and biochemical characterisation of their different nucleotide sequences and biochemical activity (14,15,19).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Wang

Yang

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A total of 68 samples were subjected to bacteria isolation on Hartley's medium. Four isolates (three from lung tissue and one from nasal discharges) were recovered from the samples and identified as the Mycoplasma species by their morphological and biochemical characteristics. They were further confirmed by PCR using 16S rRNA specific primer pairs and by restriction enzyme analysis. In vitro antimicrobial susceptibility of the isolates indicated that some strains had developed resistance to the antibiotics tested. This is the first report on the isolation, identification, and molecular characterisation of Mycoplasma species isolated from goats in Chongqing. This study also revealed a prevalence of Mycoplasma species infection in goats in this area.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Wang

Yang

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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“…A number of mycoplasma have been reported as cause of mycoplasmosis in sheep and goats in India causing various diseases (Thiaucourt & Bolske, 1996;Kumar et al, 2013;Chakraborty et al, 2014;Jain et al, 2015). Mycoplasma mycoides cluster group have five species as important pathogens for small ruminants (Nicholas et al, 2009;Chakraborty et al, 2014 (MansoSilván et al, 2009;Kumar et al, 2013;Chakraborty et al, 2014).…”

Section: Etiologiesmentioning

confidence: 99%

“…Mycoplasma mycoides cluster group have five species as important pathogens for small ruminants (Nicholas et al, 2009;Chakraborty et al, 2014 (MansoSilván et al, 2009;Kumar et al, 2013;Chakraborty et al, 2014).…”

Section: Etiologiesmentioning

confidence: 99%

Mycoplasmosis in Small Ruminants in India: A Review

Yatoo¹,

Kashmir²,

Parray³

et al. 2018

JEBAS

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Mycoplasmosis, the diseases caused by mycoplasma, are one of the challenging and continuous threats to small ruminant farming. They cause heavy morbidity (upto 100%), mortality (10-100%) and huge economic loss. Common diseases caused by mycoplasma organisms are contagious caprine pleuropneumonia (mostly in goats), contagious agalactia (both sheep and goats), atypical pneumonia (usually sheep), besides arthritis, mastitis, seminal vesiculitis, ampullitis, epididymitis, orchitis, urethritis, conjunctivitis and meningitis either alone or as classical syndromes. The common mycoplasma includes Mycoplasma mycoides subspecies mycoides, M. mycoides subsp. capri, M. capricolum subsp. capripneumoniae, M. capricolum subsp. capricolum, M. agalactiae, M. bovis and M. conjunctivae etc. Most of the mycoplasmas produce a conglomerate of clinical signs hampering diagnosis. The other main challenge posed by mycoplasma is the difficulty in isolation due to lack of

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“…Although ARDRA analysis of the 16S-23S rDNA does not seem to have potential for intraspecific differentiation of S. aureus, it was a rapid and technically relatively simple method to recognize other bacterial strains as Mycoplasma mycoides (KUMAR et al, 2013) and other bacteria (DOBNER et al, 1996;ROTH et al, 2000;JENG et al, 2001;BORSODI et al, 2015;ÖZTÜRK;METERELLIYÖZ, 2015). ARDRA has been previously used to detect intraspecific variation among high-level gentamicin-resistant enterococci (Enterococcus faecalis) (INGIANNI et al, 1997).…”

Section: Ardra Genotyping Of S Aureusmentioning

confidence: 99%

Occurrence and genetic characterization of Staphylococcus aureus in milk samples of cattle with mastitis, and in the Veterinary Hospital personnel and dairy workers

Leigue

Hilgert

Fiorini

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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Staphylococcus aureus está entre os microrganismos que apresentam as maiores taxas de morbidade e mortalidade em seres humanos e animais. Linhagens de S. aureus resistentes a meticilina podem causar surtos de infecção em todo o mundo, o que contribui para a escassez de arsenal terapêutico. Este trabalho analisou o perfil epidemiológico de estirpes de S. aureus isoladas de pessoas que trabalham em contato com animais em um hospital veterinário com gado leiteiro e também em amostras de leite de vacas acometidas por mastite. As estirpes de S. aureus isoladas foram caracterizadas fenotipicamente por meio de antibiograma, testes de catalase e coagulase, e pelo teste de Voges-Proskauer. As amostras também foram caracterizadas genotipicamente pela técnica de Analise de Restricao de DNA Ribossomico Amplificado (ARDRA-PCR). Das 218 estirpes isoladas, 27 foram identificados como S. aureus (12%). Entre essas, quatro estirpes foram resistentes a oxacilina e duas classificadas como SARM (S. aureus resistente a meticilina). A ocorrência de estirpes de S.aureus isoladas entre o pessoal que trabalha em contato com os animais foi baixa (2%), mas estirpes identificadas como SARM foram encontradas na equipe clínica. As análises de ARDRA realizadas com as enzimas de restrição HindIII e HinfII demonstraram que S. aureus isolados de diferentes indivíduos que trabalhavam com animais foram agrupados no mesmo cluster. Quando a ARDRA foi realizada com HaeIII foi observada formação de dois grupos, mas os isolados não se correlacionaram. Conclusão: a ocorrência de estirpes de S. aureus isoladas foi maior na equipe clínica, apresentando também correlação de 100% nos ensaios bioquímicos e moleculares.

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Isolation and Characterization of Mycoplasma mycoides Subspecies capri from Milk of Natural Goat Mastitis Cases

Cited by 18 publications

References 15 publications

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Mycoplasmosis in Small Ruminants in India: A Review

Occurrence and genetic characterization of Staphylococcus aureus in milk samples of cattle with mastitis, and in the Veterinary Hospital personnel and dairy workers

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