Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Wang, Haoju; Ni, Li; Yang, Hongjun; Xu, Limin; Ma, Ning; Ding, Honglei

doi:10.2478/bvip-2014-0002

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…2013; Wang et al. 2014). Fever induced anorexia is usual and in pregnant animals abortion is occasional (Sadique et al.…”

Section: Clinical Signsmentioning

confidence: 99%

“…2013; Wang et al. 2014). Clinical signs of CCPP typically correlate to respiratory system affection and these signs are common in susceptible herds and are independent of age and sex (Shah et al.…”

Section: Clinical Signsmentioning

confidence: 99%

“…2011; Wang et al. 2014). In Europe, South, Central and North America and Oceania, this disease has not been reported or information is not available (OIE 2009; EFSA AHAW Panel et al.…”

Section: Introductionmentioning

confidence: 99%

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Contagious caprine pleuropneumonia – a comprehensive review

Yatoo

Parray

Bashir

et al. 2019

Veterinary Quarterly

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Contagious caprine pleuropneumonia (CCPP) is a serious disease of goats, occasionally sheep and wild ruminants, caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). The disease is characterized by severe serofibrinous pleuropneumonia, very high morbidity ($100%), and mortality (80-100%). CCPP affects goats in more than 40 countries of the world thereby posing a serious threat to goat farming around the globe. The characteristic clinical signs of CCPP are severe respiratory distress associated with sero-mucoid nasal discharge, coughing, dyspnea, pyrexia, pleurodynia, and general malaise. In later stages, severe lobar fibrinous pleuropneumonia, profuse fluid accumulation in pleural cavity, severe congestion of lungs and adhesion formation is observed. Mycoplasmal antigen interactions with host immune system and its role in CCPP pathogenesis are not clearly understood. CCPP is not a zoonotic disease. Diagnosis has overcome cumbersome and lengthy conventional tests involving culture, isolation, and identification by advanced serological (LAT, cELISA) or gene-based amplification of DNA (PCR, RFLP, and hybridization) and sequencing. The latex agglutination test (LAT) is rapid, simple, and better test for field and real-time diagnosis applicable to whole blood or serum and is more sensitive than the CFT and easier than the cELISA. Moreover, the studies on antibiotic sensitivity and exploration of novel antibiotics (fluoroquinolones, macrolides) can help in better therapeutic management besides preventing menace of antibiotic resistance. Re-visiting conventional prophylactic measures focussing on developing novel strain-based or recombinant vaccines using specific antigens (capsular or cellular) should be the most important strategy for controlling the disease worldwide.

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“…2013; Wang et al. 2014). Fever induced anorexia is usual and in pregnant animals abortion is occasional (Sadique et al.…”

Section: Clinical Signsmentioning

confidence: 99%

Section: Clinical Signsmentioning

confidence: 99%

See 1 more Smart Citation

Contagious caprine pleuropneumonia – a comprehensive review

Yatoo

Parray

Bashir

et al. 2019

Veterinary Quarterly

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“…Approximately 507 million US dollars are lost yearly in endemic areas due to morbidity, mortality, and loss of production, in addition to costs involved in the prevention, control, and treatment [4,14]. Recent emergence of CCPP in newer areas, like Afghanistan [24], Mauritius [22], Tajikistan [25], Pakistan [26], India [4,5], China [27,28], Saudi Arabia [29], and Qatar [30], as well as increased incidence of outbreaks in prevalent areas like Ethiopia [31], Kenya [32,33], Tanzania [34], Turkey [35], and the African Union [1] has also raised concerns. Outbreaks are even occurring in wild animals [36,37,38], endangering the neighboring countries as there is always a risk of spreading the disease [5,39,40].…”

Section: Need For Ccpp Vaccinesmentioning

confidence: 99%

Novel Candidates for Vaccine Development Against Mycoplasma Capricolum Subspecies Capripneumoniae (Mccp)—Current Knowledge and Future Prospects

et al. 2019

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Exploration of novel candidates for vaccine development against Mycoplasma capricolum subspecies capripneumoniae (Mccp), the causative agent of contagious caprine pleuropneumonia (CCPP), has recently gained immense importance due to both the increased number of outbreaks and the alarming risk of transboundary spread of disease. Treatment by antibiotics as the only therapeutic strategy is not a viable option due to pathogen persistence, economic issues, and concerns of antibiotic resistance. Therefore, prophylactics or vaccines are becoming important under the current scenario. For quite some time inactivated, killed, or attenuated vaccines proved to be beneficial and provided good immunity up to a year. However, their adverse effects and requirement for larger doses led to the need for production of large quantities of Mccp. This is challenging because the required culture medium is costly and Mycoplasma growth is fastidious and slow. Furthermore, quality control is always an issue with such vaccines. Currently, novel candidate antigens including capsular polysaccharides (CPS), proteins, enzymes, and genes are being evaluated for potential use as vaccines. These have shown potential immunogenicity with promising results in eliciting protective immune responses. Being easy to produce, specific, effective and free from side effects, these novel vaccine candidates can revolutionize vaccination against CCPP. Use of novel proteomic approaches, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis, immunoblotting, matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry, tandem mass spectroscopy, fast protein liquid chromatography (FPLC), bioinformatics, computerized simulation and genomic approaches, including multilocus sequence analysis, next-generation sequencing, basic local alignment search tool (BLAST), gene expression, and recombinant expression, will further enable recognition of ideal antigenic proteins and virulence genes with vaccination potential.

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“…The type strain of MBG7 is PG50, previously known as N29, was first isolated from joint fluids of arthritic calves [ 3 ]. MBG7 has been described as a chimera between Mycoplasma capricolum and Mycoplasma mycoides [ 4 , 5 ], and group of MBG7 strains remained unassigned [ 6 ]. However, MBG7 has been assignment as a separate species, namely, Mycoplasma leachii sp.…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of P67 protein of Mycoplasma leachii

et al. 2017

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Aim:The present study was undertaken to clone, express and study the immunogenicity of P67 protein of Mycoplasma leachii.Materials and Methods:P67 gene was amplified from genomic DNA of M. leachii. The polymerase chain reaction (PCR) product was inserted in pRham N-His SUMO Kan vector and was used to transform competent Escherichia cloni 10G cells. Recombinant protein expression was done by inducing cells with 0.2% Rhamnose. Purification was done using nickel nitrilotriacetic acid affinity chromatography. Western blot and dot blot analysis were performed to assess the immunoreactivity of P67 protein.Results:PCR amplicon size of P67 gene was found to be 1500 base pair. The size of the fusion protein with SUMO tag was 79 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The recombinant P67 fusion protein expressed in pRham N-His SUMO Kan vector was found to be immunogenic in both western blot and dot blot analysis.Conclusion:Western blot and dot blot analysis of P67 protein of M. leachii revealed that the protein is immunogenic. Further work is needed to evaluate the role of P67 antigen of M. leachii as an immunodiagnostic agent.

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Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Cited by 7 publications

References 25 publications

Contagious caprine pleuropneumonia – a comprehensive review

Contagious caprine pleuropneumonia – a comprehensive review

Novel Candidates for Vaccine Development Against Mycoplasma Capricolum Subspecies Capripneumoniae (Mccp)—Current Knowledge and Future Prospects

Cloning and expression of P67 protein of Mycoplasma leachii

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