Isolation and characterization of infectious bursal disease virus (IBDV) field strains and pathotypes in Ghana

Amakye-Anim, J.; Otsyina, H. R.; Osei-Somuah, A.; Aning, K. G.

doi:10.4314/gjas.v41i2.48792

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“…A study done in Bangladesh to compare isolation of IBDV using commercial hybrid chicken embryos and indigenous chicken embryos [ 15 ] showed that rural indigenous chicken eggs were better for virus isolation than commercial farm hybrid chicken eggs. Strains of IBDV circulating in North Africa [ 16 , 17 ] and West Africa [ 18 ] have been isolated for characterization and vaccine development but not in East Africa. Currently, there is no report available on isolation of local IBDV strains using embryonated indigenous chicken eggs in Kenya.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Infectious Bursal Disease Virus Using Indigenous Chicken Embryos in Kenya

Mutinda

Njagi

Nyaga

et al. 2015

International Scholarly Research Notices

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Infectious bursal disease virus (IBDV) isolates were recovered from outbreaks to initiate activities towards developing a local vaccine strain. Use of indigenous chicken embryos was exploited to determine their potential, promote utilization of local resources for research, and enhance household economic activities. Bursa of Fabricius (BFs) samples from outbreaks shown to be IBDV positive was homogenized and inoculated in 4-week-old specific pathogen-free (SPF) IBDV seronegative white leghorn chicks. The harvested virus was inoculated into 11-day-old indigenous chicken embryos that were IBDV seronegative and passaged serially three times after which they were inoculated into 4-week-old indigenous chicks to test for presence and virulence of propagated virus. Out of 153 BFs collected from outbreaks, 43.8% (67/153) were positive for IBDV antigen and 65.7% (44/67) caused disease in SPF chicks. The embryo mean mortalities were 88% on primary inoculation, 94% in 1st passage, 91% in 2nd passage, and 67% in 3rd passage. After the third passage in embryos all the 44 isolates were virulent in 4-week-old indigenous chicks. The results show that indigenous chicken embryos support growth of IBDV and can be used to propagate the virus as an alternative viral propagating tool for respective vaccine preparation.

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Section: Introductionmentioning

confidence: 99%

Isolation of Infectious Bursal Disease Virus Using Indigenous Chicken Embryos in Kenya

Mutinda

Njagi

Nyaga

et al. 2015

International Scholarly Research Notices

View full text Add to dashboard Cite

show abstract

Isolation and characterization of infectious bursal disease virus (IBDV) field strains and pathotypes in Ghana

Abstract: Isolation and characterisation of Ghanaian field IBD virus were undertaken as part of a comprehensive study to establish an efficacious vaccination programme against the disease in the country. Bursal homogenates were

Cited by 1 publication

References 10 publications

Isolation of Infectious Bursal Disease Virus Using Indigenous Chicken Embryos in Kenya

Isolation of Infectious Bursal Disease Virus Using Indigenous Chicken Embryos in Kenya

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