Isolation and Characterization of Larvicidal Extracellular  Polysaccharide (EPS) from Pseudomonas aeruginosa B01

Benit, N.; Roslin, A. Stella

doi:10.20546/ijcmas.2018.701.013

Cited by 10 publications

(2 citation statements)

References 46 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extracted EPS was recovered using centrifugation and was allowed to dry to a constant weight for its quantification. The EPS production is represented in terms of g % w/v 23 . The recovery of EPS was optimized using various solvents in different concentrations, 1:1, 1:2 and 1:3 v/v (Fig.…”

Section: Methodsmentioning

confidence: 99%

Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Shukla

Parmar

Goswami

et al. 2021

Sci Rep

View full text Add to dashboard Cite

It is the acquisition of unique traits that adds to the enigma of microbial capabilities to carry out extraordinary processes. One such ecosystem is the soil exposed to radionuclides, in the vicinity of atomic power stations. With the aim to study thorium (Th) tolerance in the indigenous bacteria of such soil, the bacteria were isolated and screened for maximum thorium tolerance. Out of all, only one strain AM3, found to tolerate extraordinary levels of Th (1500 mg L−1), was identified to be belonging to genus Providencia and showed maximum genetic similarity with the type strain P. vermicola OP1T. This is the first report suggesting any bacteria to tolerate such high Th and we propose to term such microbes as ‘thoriotolerant’. The medium composition for cultivating AM3 was optimized using response surface methodology (RSM) which also led to an improvement in its Th-tolerance capabilities by 23%. AM3 was found to be a good producer of EPS and hence one component study was also employed for its optimization. Moreover, the EPS produced by the strain showed interaction with Th, which was deduced by Fourier Transform Infrared (FTIR) spectroscopy.

show abstract

Section: Methodsmentioning

confidence: 99%

Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Shukla

Parmar

Goswami

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Other protein precipitating agents (Greenberg & Shipe, 1979) such as 12 M HCl (Enikeev, 2012;Khanal & Lucey, 2017) 5-sulfosalycilic acid (SSA) (Pintado, Pintado, & F. Malcata, 1999a;Pintado, Pintado, & Malcata, 1999b) or ammonium sulphate (Peng, Han, Liu, & Zhou, 2016), have also been successfully introduced. After the EPS precipitation, the last step of EPS purification generally includes the resuspension of resulting material in deionized water and its dialysis against water for 1 to 7d at 4°C to remove low-molecular-mass contaminating carbohydrates that may be present in the material, and finally the resulting EPS is lyophilized (Abid et al, 2018;Benit & Roslin, 2018;London et al, 2014). The amount and composition of the isolated EPS have been shown to vary with the type of adopted approach (Mende et al, 2016;Miao, 2015), and a systematic assessment of the most effective methods for removing media contaminants remains to be done (Nguyen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media

Pintado

Ferreira

Pintado

et al. 2020

Carbohydrate Polymers

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Bioreduction of toxicity influenced by bioactive molecules secreted under metal stress by Azotobacter chroococcum

et al. 2019

View full text Add to dashboard Cite

Isolation and Characterization of Larvicidal Extracellular Polysaccharide (EPS) from Pseudomonas aeruginosa B01

Cited by 10 publications

References 46 publications

Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Efficiency of purification methods on the recovery of exopolysaccharides from fermentation media

Bioreduction of toxicity influenced by bioactive molecules secreted under metal stress by Azotobacter chroococcum

Contact Info

Product

Resources

About